Paternity analysis and pollen flow for open-pollination progenies of Hippophae rhamnoides ssp. sinensis Rousi, a constructive species of shrub from the upper reaches of Heihe River
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摘要: 利用SSR分子标记对中国沙棘（Hippophae rhamnoides ssp. sinensis Rousi）自由授粉的种子进行父本分析，研究其子代的父本来源和花粉散布模式。结果显示：在80%的置信水平上，193个子代中有104个个体可以确定花粉来源；在20个确定的父本中，16个为中国沙棘，4个为肋果沙棘（H.neurocarpa S.W.Liu et T.N.He）。传粉格局分析结果显示，中国沙棘有效花粉散布范围为3~71 m，平均距离为20.4 m，2株母本分别有87.23%和78.95%的有效花粉来自距其30 m的范围之内，研究结果表明中国沙棘自然种群以近距离传粉为主。此外，在黑河上游沙棘杂交带中，中国沙棘子代中的肋果沙棘花粉平均贡献率达到14.84%，表明中国沙棘与肋果沙棘存在较高的种间当代花粉流。Abstract: To explore the paternal origin of offspring and the pollen dispersal pattern in natural populations under open-pollination, we conducted paternity analysis for seeds of H. rhamnoides ssp. sinensis using SSR markers. Results showed that in 104 seedlings of 193 open-pollinated offspring, the pollen source was identified at an 80% confidence level. In 20 male parents deduced, 16 were H. rhamnoides ssp. sinensis and four were H. neurocarpa S. W. Liu et T. N. He. Pollination pattern analysis showed that the effective distance of H. rhamnoides ssp. sinensis pollen dispersal was in the range of 3-71 m, with an average distance of 20.4 m. Furthermore, 87.23% and 78.95% of the effective pollen received by the two respective female parents examined came from a distance range of 30 m. These results indicate that the natural populations of H. rhamnoides ssp. sinensis are mainly pollinated within a close range. In addition, in the seabuckthorn hybrid zone of the upper reaches of Heihe River, the average pollen contribution from H. neurocarpa to the offspring of H. rhamnoides ssp. sinensis reaches 14.84%, indicating that H. rhamnoides ssp. sinensis and H. neurocarpa possess a relatively high frequency of contemporary interspecific pollen flow.
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