De novo assembly and transcriptome analysis of Hylocereus undulatus during development
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摘要: 利用高通量测序技术对火龙果（Hylocereus undulatus Britt）红肉品种‘大红二号’的花芽、果实和枝条不同发育阶段的基因表达进行研究。结果显示，转录组测序共获得468.68 Gb原始数据（Raw data），从头组装获得239 152条转录本和162 519条unigene，约53.74%的unigene得到注释。分别在43 506条和16 251条unigene中检测到600 283个SNP位点和56 147个SSR位点。基因表达分析结果表明，在火龙果不同组织Fl510、Fl513、Fl514、Fl518、F711、F715、S513、S419中分别有31、7、5、152、17、63、17、8个特异表达的unigene。通过GO和KEGG富集分析，发现了一些组织特异的GO条目和代谢通路，如在Fl510中富集的类萜骨架生物合成代谢通路等。本研究还对参与花发育的候选基因进行了鉴定和表达分析，他们包括COL基因、FT-like基因、分生组织决定基因和器官决定基因等。Abstract: High-throughput sequencing was used to analyze the gene expression of the reproductive buds, fruits, and shoots of Hylocereus undulatus Britt ‘Dahong2’ at different developmental stages. In total, 468.68 Gb of raw data were generated and de novo assembled into 239 152 transcripts and 162 519 unigenes. Approximately 53.74% of all unigenes were annotated based on seven public databases. In total, 600 283 SNPs and 56147 SSRs were identified from 43 506 and 16 251 unigenes, respectively. Gene expression analysis showed that 31, 7, 5, 152, 17, 63, 17, and 8 unigenes were specifically expressed in Fl510, Fl513, Fl514, Fl518, F711, F715, S513, and S419, respectively. Through GO and KEGG enrichment analyses, several unique GO terms and metabolic pathways in different tissues were identified; for example, terpenoid backbone biosynthesis (ko00900) showed significant enrichment in Fl510. We also focused on the molecular mechanism of H.undulatus ‘Dahong2’ flower development and identified a suite of unigenes involved in floral development, including COL, FT-like, meristem identity, and organ identity genes.
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Keywords:
- Hylocereus undulatus /
- Transcriptome sequencing /
- Growth /
- Floral development /
- Gene expression
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