应用流式细胞术测定绢毛苣属4种8居群基因组大小

杨慧娴; 饶培瑜; 胡晶晶; 欧晓昆; 李志敏

doi:10.11913/PSJ.2095-0837.2019.60775

应用流式细胞术测定绢毛苣属4种8居群基因组大小

1. 云南师范大学生命科学学院, 昆明 650500;
2. 云南大学生态学与环境学院, 生态学与地植物研究所, 昆明 650500

基金项目:

国家自然科学基金项目（31670206，31960046）；云南省科技厅重大项目（2018FY001-002）。

详细信息

作者简介:
杨慧娴(1995-),女,硕士研究生,研究方向为植物细胞地理学(E-mail:yanghuixian77@163.com)。

通讯作者:
李志敏,E-mail:lizhimin_vip@163.com

中图分类号: Q943;949.783.5
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出版历程
- 收稿日期: 2019-04-06
- 修回日期: 2019-06-16
- 网络出版日期: 2022-10-31
- 发布日期: 2019-12-27

Estimation of genome size from eight populations of four species of Soroseris Stebbins by flow cytometry

1. School of Life Science, Yunnan Normal University, Kunming 650500, China;
2. Institute of Ecology and Geobotany, School of Ecology and Environment, Yunnan University, Kunming 650500, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31670206, 31960046) and Project of Yunnan Natural Science (2018FY001-002).

摘要

摘要: 以绢毛苣属（Soroseris）植物肉菊（S.umbrella（Franch.）Lipsch）、绢毛苣（S.glomerata（Decne.）Stebbins）、羽裂绢毛苣（S.hirsuta（Anth.）Shih）和金沙绢毛菊（S.gillii（S.Moore）Stebbins）4个种的8个居群为材料，采用WPB和Galbraith's buffer两种裂解液分别处理样品，运用流式细胞术测定DNA含量，并使用常规染色体压片法确定其细胞倍性。结果显示：两种裂解液中，最适于绢毛苣属植物DNA含量测定的裂解液为WPB；4个物种中，羽裂绢毛苣的基因组最小；细胞倍性水平对1Cx-值的影响较小。此外，本研究发现可采用种子萌发形成的幼苗代替难以获取的植物幼嫩叶片作为实验材料。
- 绢毛苣属 /
- 流式细胞术 /
- 基因组大小 /
- 倍性
Abstract: In this study, eight populations of four Soroseris Stebbins species were used as experimental materials. The DNA content was determined by flow cytometry based on WPB and Galbraith's buffer and ploidy was determined by traditional chromosome tableting. Results showed that, after comparing the two dissociation buffers, WPB was found to be the most suitable for Soroseris. In addition, S. hirsuta (Anth.) Shih had the smallest genome and the polyploidy level had little effect on the 1Cx-value in the four species. Furthermore, this study also found that seedlings formed by seed germination could be used as experimental materials instead of young leaves that are difficult to obtain.
- Soroseris /
- Flow cytometry /
- Genome size /
- Polyploidy

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