银杏胚乳淀粉体的积累规律和发生特性

陆彦; 郝唯卓; 潘烨; 张晓敏; 祁琰; 凌裕平; 金飚; 王莉

doi:10.11913/PSJ.2095-0837.2019.60788

银杏胚乳淀粉体的积累规律和发生特性

陆彦^1,3,
郝唯卓^1,3,
潘烨²,
张晓敏^1,2,
祁琰^1,2,
凌裕平²,
金飚²,
王莉^2, ,

1. 扬州大学测试中心, 江苏扬州 225009;
2. 扬州大学园艺与植物保护学院, 江苏扬州 225009;
3. 扬州大学农学院, 江苏扬州 225009

基金项目:

国家自然科学基金项目（31901517）；江苏省自然科学基金项目（BK20160464，BK20161332）；中国博士后科学基金面上项目（2019M651980）；江苏省林业科技创新与推广项目（LYKJ[2018]39）。

详细信息

作者简介:
陆彦(1981-),女,博士,助理研究员,研究方向为植物发育生物学(E-mail:luyan@yzu.edu.cn)。

通讯作者:
王莉,E-mail:liwang@yzu.edu.cn

中图分类号: Q944.46
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出版历程
- 收稿日期: 2019-07-13
- 修回日期: 2019-08-05
- 网络出版日期: 2022-10-31
- 发布日期: 2019-12-27

Accumulation and occurrence characteristics of amyloid in Ginkgo biloba L. endosperm

Lu Yan^1,3,
Hao Wei-Zhuo^1,3,
Pan Ye²,
Zhang Xiao-Min^1,2,
Qi Yan^1,2,
Ling Yu-Ping²,
Jin Biao²,
Wang Li^2, ,

1. Instrumental Analysis Center, Yangzhou University, Yangzhou, Jiangsu 225009, China;
2. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, China;
3. Agricultural College, Yangzhou University, Yangzhou, Jiangsu 225009, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31901517), Jiangsu Natural Science Foundation (BK20160464, BK20161332), China Postdoctoral Science Foundation (2019M651980), and Jiangsu Forestry Science and Technology Innovation and Popularization Project (LYKJ[2018]39).

摘要

摘要: 以银杏（Ginkgo biloba L.）核用品种‘七星果’、‘马铃’和‘龙眼’不同发育天数的胚乳为材料，采用透射电镜和扫描电镜技术，对其胚乳细胞内淀粉体的积累规律和发生特性进行研究。结果显示：3种银杏胚乳形态差异显著，‘七星果’呈梭形、‘马铃’呈椭圆形、‘龙眼’呈卵圆形；3种银杏胚乳早期均为嫩绿色，后期为黄色；授粉后65~125 d是胚乳体积快速增长时期。淀粉体的积累规律为：在胚乳组织内，淀粉体由糊粉层-外胚乳-内胚乳逐渐积累；在单个胚乳细胞内，淀粉体由细胞壁边缘向内部逐渐充实。银杏淀粉质体起源于类叶绿体质体，淀粉粒最初在类叶绿体质体的内膜上发生。淀粉体通过出芽、缢缩以及出芽和缢缩同时进行的增殖方式产生新淀粉体，成熟淀粉体形态有圆形、椭圆形和不规则形，属于单粒淀粉。研究结果表明银杏淀粉体在胚乳组织内具有由外向内的空间积累规律，淀粉质体起源于类叶绿体质体并通过出芽、缢缩、出芽和缢缩同时存在的方式增殖。
- 银杏 /
- 胚乳 /
- 淀粉体 /
- 积累规律 /
- 发生特性
Abstract: In this study, three Ginkgo biloba L. cultivars, namely, ‘ixingguo’, ‘Maling’ and ‘Longyan’, were used to investigate the accumulation and occurrence of amyloid in the endosperm using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Results showed that endosperm morphology differed among the three ginkgo cultivars, being fusiform, oval, and ovoid in ‘Qixingguo’, ‘Maling’ and ‘Longyan’, respectively. The endosperms from the three cultivars were green in the early developing stage and yellow in the later developing stage. Rapid endosperm growth occurred from 65 d to 125 d after pollination. The accumulation of starch grains in the endosperm cells showed a spatial accumulation pattern, with gradual enrichment by the aleurone layer-ecto-endosperm-inner endosperm from the edge of the cell wall to the cell interior. Amyloid developed from the grana lamella of chloroplast-like plastids. These amyloid proliferated by budding, contraction, or budding and contraction. The matured amyloid was single-starch and round, oval, or irregular in shape. In summary, the starch grains accumulated from the outside to the inside in the ginkgo endosperm. The amyloid originated from chloroplast-like plastids and proliferated by budding, contraction, or budding and contraction at the same time.
- Ginkgo biloba /
- Endosperm /
- Amyloid /
- Accumulation /
- Occurrence characteristics

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