Expression analysis of FaesSTK gene in Fagopyrum esculentum Moench with long pistil and stamen
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摘要: 采用RACE技术，从甜荞（Fagopyrum esculentum Moench）中克隆获得3种花型的STK同源基因FaesSTK，并对其序列特征进行分析。结果显示，甜荞3种花型植株STK同源基因序列一致，全长为967 bp，包含长689 bp的完整开放阅读框，编码一个由225个氨基酸残基组成的D类MADS-box转录因子。蛋白序列比对及系统发育分析结果表明，FaesSTK蛋白属于MADS-box转录因子中的STK进化系。包含1个由57个氨基酸残基组成的高度保守的MADS结构域；1个由82个氨基酸残基组成的次级保守区域的K结构域，在C端的转录激活区还含有另外2个高度保守的基序（AGⅠ和AGⅡ）。实时荧光定量检测结果显示，FaesSTK基因主要在甜荞lpls突变体的雄蕊、雌蕊和不同发育时期的幼果中表达，在根和花被片中仅能检测到微弱的转录信号，在叶和茎中不表达，其中在雌蕊和果实中的表达量极显著高于其他组织。推测该基因在花发育过程中可能主要参与调控甜荞lpls突变体雌蕊和果实的发育。Abstract: Using RACE technology, three flower types of STK homologous gene FaesSTK (GenBank accession number:MN597104) were examined from Fagopyrum esculentum Moench, and their sequence characteristics were analyzed. Sequence alignment results suggested that the sequences of the gene from the three flower types were identical. The gene was 967 bp in length and contained a 689 bp open reading frame (ORF) encoding 225 amino acids. Protein sequence alignment and phylogenetic analyses grouped the FaesSTK protein into the STK linage of D-class MADS-box transcription factors. FaesSTK contained a highly conservation MADS domain with 57 amino acids, a secondary conserved K-domain with 82 amino acids, as well as two highly conserved motifs (AGⅠ motif and AGⅡ motif) in the variable C-terminal region. In addition, quantitative real-time polymerase chain reaction (qRT-PCR) revealed that the FaesSTK gene was mainly expressed in the stamen, gynoecium, and young fruits at different developmental stages in the F. esculentum lpls mutant. Moreover, FaesSTK was weakly transcribed in the root and tepal but was absent in the leaf and stem. FaesSTK expression levels in the gynoecium and fruit were significantly higher than that in other tissues. Our data suggest that FaesSTK may play a major role in the development of the gynoecium and fruit of the F. esculentum lpls mutant.
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Keywords:
- Fagopyrum esculentum /
- Flower development /
- FaesSTK
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