非洲白参的抗氧化活性及其化学成分研究

甄兆孟; 庄小翠; 刘叶; 陈桂林; 郭明全

doi:10.11913/PSJ.2095-0837.2020.40543

非洲白参的抗氧化活性及其化学成分研究

甄兆孟^1,2,4,
庄小翠^1,2,4,
刘叶^1,2,3,
陈桂林^1,2,3,
郭明全^1,2,3, ,

1. 中国科学院武汉植物园植物种质创新与特色农业重点实验室, 武汉 430074;
2. 中国科学院中-非联合研究中心, 武汉 430074;
3. 中国科学院药物创新研究院, 上海 201203;
4. 中国科学院大学, 北京 100049

基金项目:

国家自然科学基金项目（81903791）；湖北省对外科技合作技术创新专项（2017AHB054）；湖北省自然科学基金项目（2019CFB254）。

详细信息

作者简介:
甄兆孟(1995-),女,硕士研究生,研究方向为植物化学(E-mail:zhenzhaomeng19@163.com)。

通讯作者:
郭明全,E-mail:guomq@wbgcas.cn

中图分类号: R284.1
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出版历程
- 收稿日期: 2019-11-29
- 修回日期: 2020-01-11
- 网络出版日期: 2022-10-31
- 发布日期: 2020-08-27

Antioxidant activities and chemical constituents of roots of Mondia whiteii (Hook. f.) Skeels

Zhen Zhao-Meng^1,2,4,
Zhuang Xiao-Cui^1,2,4,
Liu Ye^1,2,3,
Chen Gui-Lin^1,2,3,
Guo Ming-Quan^1,2,3, ,

1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
2. Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China;
3. Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China;
4. University of Chinese Academy of Sciences, Beijing 100049, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (81903791), Major Project for Special Technology Innovation of Hubei Province-Sino-Foreign Cooperation Project (2017AHB054), and Natural Science Foundation of Hubei Province (2019CFB254).

摘要

摘要: 采用DPPH（1，1-二苯基-2-三硝基苯肼）、ABTS（2，2-联氮-双-3-乙基苯并噻唑-6-磺酸）自由基清除法和FRAP（铁离子还原/抗氧化能力）法对非洲白参（Mondia whiteii（Hook.f.）Skeels）不同萃取部位的抗氧化活性进行分析，然后采用紫外分光光度法和福林酚比色法分别测定非洲白参不同萃取部位的总黄酮、总酚含量，最后采用大孔树脂AB-8、ODS以及Sephadex LH-20柱层析和半制备型HPLC等色谱分离技术对其二氯甲烷萃取部位的化学成分进行分离、纯化。结果显示：非洲白参二氯甲烷萃取部位抗氧化活性最高，且二氯甲烷萃取部位的总酚含量远高于其他部位；化学成分分离、纯化后得到10个单体化合物，分别为：2-羟基-4-甲氧基苯甲醛（1）、秦皮素（2）、7-甲氧基香豆素（3）、α-羟基丁香丙酮（4）、ω-hydroxypropioguaiacone（5）、桂皮酸（6）、水杨酸（7）、4-甲氧基水杨酸（8）、丁香酸（9）、壬二酸（10）。其中，化合物3~10为首次从该植物中分离得到。
- 非洲白参 /
- 抗氧化 /
- 化学成分 /
- 分离纯化
Abstract: The antioxidant activities of different fractions of Mondia whiteii (Hook. f.) Skeels were measured by using three different assays (DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS(2,2'-azinobis-3-ethylbenzthiazoline-6-sulfonic acid) and FRAP (Ferric-Reducing Antioxidant Power), respectively). Total flavonoid and total phenol contents were also tested, and the chemical constituents of the dichloromethane fraction were further isolated and purified by AB-8 macroporous resin column chromatography (CC), Octadecylsilyl(ODSCC), Sephadex LH-20CC, and preparative-HPLC(High Performance Liquid Chromatography). Results showed that the dichloromethane fraction had the highest antioxidant activities and total phenol content. Furthermore, 10 compounds were obtained and identified, including 2-hydroxy-4-methoxybenzaldehyde(1), fraxetin(2), 7-metho-xycoumarin (3), α-hydroxypropiosyringone (4), ω-hydroxypropioguaiacone (5), cinnamic acid (6), salicylic acid (7), 4-methoxysalicylic acid (8), syringic acid (9), and azelaic acid (10). To the best of our knowledge, this study is the first to isolate compounds 3-10 from Mondia whiteii.
- Mondia whiteii /
- Antioxidant activities /
- Chemical constituents /
- Isolation

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