基于RNA-seq技术的青藏高原不同海拔水毛茛转录组测序及基因表达分析

陈飞飞; 姬亚丽; 陈忠海; 刘泰龙; 刘星

doi:10.11913/PSJ.2095-0837.2021.10050

基于RNA-seq技术的青藏高原不同海拔水毛茛转录组测序及基因表达分析

陈飞飞^1,2,
姬亚丽^1,2,
陈忠海^1,2,
刘泰龙^1,2,
刘星^1,2,3, ,

1. 西藏大学理学院, 拉萨 850000;
2. 西藏大学青藏高原生态与环境研究中心极端环境生物适应与进化实验室, 拉萨 850000;
3. 武汉大学生命科学学院, 武汉 430072

基金项目:

国家自然科学基金项目（31860046）。

详细信息

作者简介:
陈飞飞(1994-),男,硕士研究生,研究方向为青藏高原生物多样性与分子进化(E-mail:1922615045@qq.com)。

通讯作者:
刘星,E-mail:xingliu@whu.edu.cn

中图分类号: Q943.2
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出版历程
- 收稿日期: 2020-06-17
- 修回日期: 2020-09-29
- 网络出版日期: 2022-10-31
- 发布日期: 2021-02-27

Transcriptome sequencing and expression analysis of Batrachium bungei (Steud.) L. Liou at different altitudes based on RNA-seq in the Qinghai-Tibet Plateau

Chen Fei-Fei^1,2,
Ji Ya-Li^1,2,
Chen Zhong-Hai^1,2,
Liu Tai-Long^1,2,
Liu Xing^1,2,3, ,

1. College of Science, Tibet University, Lhasa 850000, China;
2. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Extreme Environmental Biological Adaptation and Evolution Laboratory, Lhasa 850000, China;
3. College of Life Sciences, Wuhan University, Wuhan 430072, China

Funds:

This work was supported by a grant from the National Natural Science Foundation of China (31860046).

摘要

摘要: 以青藏高原不同海拔7个居群的水毛茛（Batrachium bungei （Steud.） L.Liou）为材料，对其进行转录组测序及基因表达分析，研究它们在极端环境下基因表达方面的适应性。结果显示，7个居群内样本间的基因表达具有较高的相似性。差异基因富集分析结果发现苯丙烷生物合成相关基因在5个差异组中均呈显著富集；此外类黄酮、类胡萝卜素、苯丙氨酸、酪氨酸、色氨酸的生物合成以及激素的信号转导、MAPK信号通路、植物与病原菌互作等相关基因大多被显著富集。与低海拔居群相比，参与类黄酮生物合成通路的相关基因（HHT1、HCT、F3'H、CHS、CYP73A、CCOAOMT5、CYP98A）在高海拔居群中均显著上调表达。研究结果表明水毛茛主要通过多途径的参与及关键基因的调控表达来适应青藏高原的高海拔环境。
- 水毛茛 /
- 转录组测序 /
- 差异表达 /
- 富集分析 /
- 类黄酮
Abstract: We explored how Batrachium bungei (Steud.) L. Liou has adapted to extreme high-altitude environments. We used transcriptome sequencing and bioinformatics analysis of seven populations of B. bungei (ML, MZ, WQ, YH, YBJ, XB, ZR). Based on Pearson correlation analysis and principal component analysis (PCA), results showed that the gene expression of each population was highly similar. Differential gene enrichment analysis showed that phenylpropanoid biosynthesis was significantly enriched in five different groups. In addition, flavonoid biosynthesis, carotenoid biosynthesis, phenylalanine tyrosine and tryptophan biosynthesis, plant hormone signaling transduction, MAPK signaling pathway, and plant-pathogen interaction were significantly enriched. Compared with low-altitude populations, flavonoid biosynthesis genes (HHT1, HCT, F3'H, CHS, CYP73A, CCOAOMT5, CYP98A) were significantly up-regulated in the high-altitude populations, and their expression levels were significantly higher than those in the low-altitude (MZ) population. These results indicate that B. bungei adapted to the high-altitude environment of the Qinghai-Tibet Plateau mainly through multi-pathway participation and the regulation and expression of key genes.
- Batrachium bungei /
- Transcriptome sequencing /
- Differential expression /
- Enrichment analysis /
- Flavonoids

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