Regional quality differences and climatic response characteristics of Codonopsis pilosula (Franch.) Nannf. in Gansu Province
-
摘要: 基于甘肃产区党参（Codonopsis pilosula（Franch.） Nannf.）中4种代表性组分（党参炔苷、苍术内酯Ⅲ、多糖和醇溶性浸出物）的含量变化与相应地域气候因子的分析，探明甘肃境内区域条件、气候因子对党参化学成分的影响及关联性。运用高效液相色谱法测定党参中这4种化学成分含量，分析区域性、年-季-月不同时间尺度的气候因子与党参4种化学成分的关系并计算出相关系数。结果显示，甘肃地区党参化学成分含量存在区域性差异，且苍术内酯Ⅲ是造成差异的主要化学成分。不同地区间降水量和日照量的差异是造成苍术内酯Ⅲ含量区域性差异的气候因素，在一定程度下降水量高且日照量较少的地区有助于苍术内酯Ⅲ的形成和积累。月降水和月均日照量对苍术内酯Ⅲ含量影响较大，其含量与3-10月月降水呈显著正相关，与3-5、7月的月均日照量呈显著负相关。党参炔苷、醇溶性浸出物含量与气候因子无显著相关性；党参多糖含量与年均温度呈显著正相关。
-
关键词:
- 党参 /
- 区域性品质 /
- 不同时间尺度气候因子 /
- 区域气候特征
Abstract: We explored regional differences in the chemical composition and climate response mechanism for the accumulation of active constituents in Codonopsis pilosula (Franch.) Nannf. based on chemical components and multi-scale climatic factors in Gansu Province. High-performance liquid chromatography (HPLC) was applied to determine the concentrations and differences in the contents of active components in C. pilosula from different regions. Correlation analysis was performed to assess the relationships among annual, quarterly, and monthly climatic factors and chemical components. Results showed regional differences in the chemical component contents in C. pilosula, with atractylenolide Ⅲ content showing the most significant difference across regions. There was no significant correlation between climatic factors and contents of lobetyolin or ethanol-soluble extractives. However, polysaccharide content was positively correlated with mean annual temperature and atractylenolide Ⅲ content was positively correlated with annual precipitation and annual average relative humidity, but negatively correlated with annual average solar radiation. Monthly mean precipitation and monthly mean solar radiation were key factors related to atractylenolide Ⅲ content in C. pilosula. Atractylenolide Ⅲ content was strongly positively related to monthly mean precipitation from March to October, but negatively correlated with monthly mean solar radiation from March to May and July. Precipitation and solar radiation were important climatic factors related to the regional differences in atractylenolide Ⅲ content. High precipitation and low solar radiation were conducive to atractylenolide Ⅲ accumulation in C. pilosula. -
-
[1] 国家药典委员会. 中华人民共和国药典:一部[S]. 北京:中国医药科技出版社, 2015:281. [2] 王晓霞, 庄鹏宇, 陈金铭, 杨宇柯, 林晓莹, 张丹阳. 党参化学成分的研究[J]. 中草药, 2017, 48(9):1719-1723. Wang XX, Zhuang PY, Chen JM, Yang YK, Lin XY, Zhang DY. Study on chemical constituents of Codonopsis pilosula[J]. Chinese Traditional and Herbal Drugs, 2017, 48(9):1719-1723.
[3] 冯亚静, 王晓霞, 庄鹏宇, 张丹阳, 高磊,等. 党参的化学成分研究[J]. 中国中药杂志, 2017, 42(1):135-139. Feng YJ, Wang XX, Zhuang PY, Zhang DY, Gao L, et al. Study on chemical constituents of Codonopsis pilosula[J]. China Journal of Chinese Materia Medica, 2017, 42(1):135-139.
[4] 郑涛, 晏永明, 徐福荣, 程永现. 潞党参化学成分研究[J]. 天然产物研究与开发, 2017, 29(10):1642-1647. Zheng T, Yan YM, Xü FR, Cheng YX. Compounds from Codonopsis pliosula[J]. Natural Product Research and Development, 2017, 29(10):1642-1647.
[5] 谢琦, 程雪梅, 胡芳弟, 王长虹. 党参化学成分、药理作用及质量控制研究进展[J]. 上海中医药杂志, 2020, 54(8):94-104. Xie Q, Cheng XM, Hu FD, Wang CH. Research advance on chemical constituents, pharmacological action and quality control of Codonopsis pilosula[J]. Shanghai Journal of Traditional Chinese Medicine, 2020, 54(8):94-104.
[6] 陈克克. 中药党参的研究概况[J]. 西安文理学院学报(自然科学版), 2008, 11(2):33-39. Chen KK. Research progress in Codonopisis pilosula[J]. Journal of Xi'an University of Arts and Science (Natural Science Edition), 2008, 11(2):33-39.
[7] 郝艳艳, 聂春霞, 武晓伟, 刘聪, 郝旭亮. 党参多糖及其结构改性对免疫调节作用影响的研究进展[J]. 中国医药导报, 2018, 15(28):25-28. Hao YY, Nie CX, Wu XW, Liu C, Hao XL. Research progress of Codonopsis pilosula polysaccharide and its structure modification on immune regulation[J]. China Medical Herald, 2018, 15(28):25-28.
[8] 李成义, 刘书斌, 李硕, 强正泽, 李俊岳, 王明伟. 甘肃党参栽培现状调查分析[J]. 中国现代中药, 2016, 18(1):102-105. Li CY, Liu SB, Li S, Qiang ZZ, Li JY, Wang MW. Investigation and analysis of status cultivation of Gansu Codonopsis pilosula[J]. Modern Chinese Medicine, 2016, 18(1):102-105.
[9] 张向东, 高建平, 曹铃亚, 针娴. 中药党参资源及生产现状[J]. 中华中医药学刊, 2013, 31(3):496-498. Zhang XD, Gao JP, Cao LY, Zhen X. Survey of Codonopsis germplasm resource and production status[J]. Chinese Archives of Traditional Chinese Medicine, 2013, 31(3):496-498.
[10] 卫亚洁, 王佳宇, 王安冬, 白光灿, 侯文珍,等. 当归的道地性评价研究现状[J]. 中医药导报, 2017, 23(5):96-98. Wei YJ, Wang JY, Wang AD, Bai GC, Hou WZ, et al. Current situation on genuineness of Danggui (Angelica sinensis (Oliv.) Diels)[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacology, 2017, 23(5):96-98.
[11] 金尧. 不同生态因子对当归产量及阿魏酸含量的影响[D]. 兰州:甘肃中医学院, 2014. [12] 耿树香, 韩明珠, 宁德鲁, 陈海云, 贺娜. 云南不同产地漾濞泡核桃品质综合评价分析[J]. 中国油脂, 2019, 44(5):156-160. Geng SX, Han MZ, Ning DL, Chen HY, He N. Comprehensive evaluation and analysis of the quality of Yangbi bubble walnut from different habitats in Yunnan[J]. China Oils and Fats, 2019, 44(5):156-160.
[13] 朱振玲, 张敏, 王梦月, 李晓波. 广西不同产地肉桂药材质量评价[J]. 山西医科大学学报, 2019, 50(4):473-479. Zhu ZL, Zhang M, Wang MY, Li XB. Quality evaluation of Cinnamomi cassia from different habitats in Guangxi[J]. Journal of Shanxi Medical University, 2019, 50(4):473-479.
[14] 李成义, 刘书斌, 王明伟, 魏学明, 强正泽, 张樱山. 不同商品等级甘肃白条党参质量比较研究[J]. 中国中医药信息杂志, 2016, 23(5):91-95. Li CY, Liu SB, Wang MW, Wei XM, Qiang ZZ, Zhang YS. Comprehensive quality evaluation of Gansu Baitiao Codonopsis pilosula with different commercial grades[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2016, 23(5):91-95.
[15] 刘书斌. 甘肃地产党参商品等级划分合理性分析及相关性的研究[D]. 兰州:甘肃中医药大学,2016. [16] 袁媛, 周骏辉, 黄璐琦. 黄芩道地性形成"逆境效应"的实验验证与展望[J]. 中国中药杂志, 2016, 41(1):139-143. Yuan Y, Zhou JH, Huang LQ. Experimental verification and prospect on stress effect of Dao-di herbs Scutellaria baicalensis[J]. China Journal of Chinese Materia Medica, 2016, 41(1):139-143.
[17] 张建清, 苏雪, 吴琼, 丁松爽, 孙坤. 药用植物党参的RAPD分析[J]. 中药材, 2006, 29(5):417-420. Zhang JQ, Su X, Wu Q, Ding SS, Sun K. Analysis of RAPD on medicinal plants of Codonopsis pilosula[J]. Journal of Chinese Medicinal Materials, 2006, 29(5):417-420.
[18] 刘璇, 徐娇, 金钺, 隋春. 党参种质资源与优良品种选育研究进展[J]. 农学学报, 2018, 8(7):36-41. Liu X, Xu J, Jin Y, Sui C. Germplasm resources and cultivar breeding of Codonopsis pilosula:research progress[J]. Journal of Agriculture, 2018, 8(7):36-41.
[19] 禹娟红. 不同产地党参药材质量评价研究进展[J]. 中国中医药科技, 2016, 23(4):503-505. Yu JH. Research progress on quality evaluation of Codonopsis pilosula from different habitats[J]. Chinese Journal of Traditional Medical Science and Technology, 2016, 23(4):503-505.
[20] 杨静, 苏强, 刘恩荔, 高建平. 不同产地党参苍术内酯Ⅲ和党参炔苷含量测定[J]. 山西医科大学学报, 2010, 41(8):698-702. Yang J, Su Q, Liu EL, Gao JP. Assay of lobetyolin and atractylenolideⅢ in Codonopsis pilosula from different habitats[J]. Journal of Shanxi Medical University, 2010, 41(8):698-702.
[21] 于玲, 范罗嫡, 胡明华, 马方励, 戚进. 甘肃不同产地党参HPLC指纹图谱研究[J]. 海峡药学, 2018, 30(10):38-40. Yu L, Fan LD, Hu MH, Ma FL, Qi J. Study on HPLC finger printing of Codonopsis pilosula from different habitats in Gansu[J]. Strait Pharmaceutical Journal, 2018, 30(10):38-40.
[22] 黄继锋, 崔治家, 侯嘉, 王明伟, 吴戈平, 杨扶德. 甘肃不同产地党参药材中党参炔苷含量测定[J]. 中兽医医药杂志, 2017, 36(2):44-47. Huang JF, Cui ZJ, Hou J, Wang MW, Wu GP, Yang FD. Determination of lobetyolin in Codonopsis pilosula in Gansu Province[J]. Journal of Traditional Chinese Veterinary Medicine, 2017, 36(2):44-47.
[23] 窦武宇, 崔治家, 侯嘉, 王明伟, 杨扶德. 甘肃不同产地党参药材中多糖含量测定[J].中兽医医药杂志, 2016, 35(3):55-59. Dou WY, Cui ZJ, Hou J, Wang MW, Yang FD. Determination of polysaccharides in Codonopsis pilosula from different habitats in Gansu Province[J]. Journal of Tradi-tional Chinese Veterinary Medicine, 2016, 35(3):55-59.
[24] 刘书斌, 李成义, 王明伟, 李硕, 强正泽, 魏学明. 甘肃地产白条党参商品等级与活性成分的相关性分析[J]. 时珍国医国药, 2016, 27(2):329-331. Liu SB, Li CY, Wang MW, Li S, Qiang ZZ, Wei XM. Correlation analysis between commodity grade and active ingredient of Codonopsis pilosula in Gansu real estate[J]. Lishizhen Medicine and Materia Medica Research, 2016, 27(2):329-331.
[25] 吴洁荣, 尹文仲, 吴瑞云. 恩施板桥党参的质量特征与产地自然条件[J]. 中南民族大学学报(自然科学版), 2009, 28(2):57-60. Wu YJ, Yin WZ, Wu RY. Quality Characteristics of Codonopsis tangshen Oliv. Produced in Enshi autonomous region Banqiao Town and natural conditions of producing area[J]. Journal of South-Central University for Nationalities (Natural Science Edition), 2009, 28(2):57-60.
[26] 吴晓俊, 张小波, 郭兰萍, 余意, 黄璐琦. 党参药材分布区划研究[J]. 中国中药杂志, 2017, 42(22):4368-4372. Wu XJ, Zhang XB, Guo LP, Yu Y, Huang LQ. Study on distribution division of Codonopsis pilosula[J]. China Journal of Chinese Materia Medica, 2017, 42(22):4368-4372.
[27] 漆文选. 高寒旱区道地优质白条党参生长环境因子与持续栽培技术[J]. 陕西农业科学, 2015, 61(4):127-129. Qi WX. Growth Environmental factors and continuous cultivation techniques of Codonopsis pilosula in alpine and arid regions[J]. Shanxi Journal of Agricultural Sciences, 2015, 61(4):127-129.
[28] 栾青, 郭建平, 马雅丽, 孙英杰, 陈虎胜, 牛玲莉. 晋东南潞党参生态气候适生种植区划[J]. 生态学杂志, 2020, 39(9):2953-2962. Luan Q, Guo JP, Ma YL, Sun YJ, Chen HS, Niu LL. Division of eco-climate planting area of Codonopsis pilosula in southeastern Shanxi[J]. Chinese Journal of Ecology, 2020, 39(9):2953-2962.
[29] 张玲. 甘草黄芪党参在甘肃的气候适应性及种植区域评述[J]. 甘肃农业科技, 2020(5):50-55. Zhang L. Climate adaptability and planting area of Glycyrrhizae uralensis, Astragalus membranaceus and Codonopsis pilosula in Gansu Province[J]. Gansu Agricultural Science and Technology, 2020(5):50-55.
[30] 常丽. 山西省长子县适宜党参生长的气候条件分析[J]. 北京农业, 2013(9):152-153. [31] 秦仁艳. 道真县洛党参种植气候条件分析[J]. 现代农业科技, 2017(24):196-198. [32] 张立军, 王宏霞, 王国祥, 蔡子平, 蔺海明, 等. 甘肃党参搭架采种技术规程[J]. 甘肃农业科技, 2018(6):92-94. [33] 何春雨, 张延红, 蔺海明. 甘肃道地党参生长动态研究[J]. 中国中药杂志, 2006, 31(4):285-289. He CY, Zhang YH, Lin HM. Study on dynamic growth of Codonopsis pilosula[J]. China Journal of Chinese Materia Medica, 2006, 31(4):285-289.
计量
- 文章访问数: 516
- HTML全文浏览量: 3
- PDF下载量: 665
下载:
