Mining and analysis of the self-incompatibility S gene in Aconitum pendulum N. Busch based on RNA-seq
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摘要: 以毛茛科乌头属铁棒锤（Aconitum pendulum N.Busch）2个品系‘蓝花铁棒锤’（‘WSYB1’）和‘黄花铁棒锤’（‘WSYY1’）为材料，对其进行转录组测序（RNA-seq），采用生物信息学方法鉴定其中可能存在的花柱S基因（self-incompatibility gene）和花粉S基因，并对它们的序列特征进行分析。结果显示，转录组中共鉴定出2个在雌蕊中特异或高表达的花柱S基因（ApSRNase）和2个在雄蕊中特异表达的花粉S基因（ApSLF）。与耧斗菜（Aquilegia coerulea James）相似，铁棒锤中也存在S-RNase（S locus ribonucleases）和SLF（S locus F-box）控制的S-RNase类的自交不亲和系统，而不存在sS（stigma S-determinant）和pS（pollen S-determinant）控制的罂粟科类型的自交不亲和系统。Abstract: Two Aconitum pendulum N. Busch cultivars (‘WSYB1’ and ‘WSYY1’) were used as materials for transcriptome sequencing (RNA-seq) using bioinformatics to identify possible style and pollen S genes and analyze their sequence characteristics. Results identified two style S genes (ApSRNase) specifically or highly expressed in the style and two pollen S genes (ApSLF) specifically expressed in pollen. Similar to the sequenced plant Aquilegia coerulea James, this study showed that there was a S-RNase-based self-incompatibility system controlled by S-RNase and SLF in A. pendulum, but no Papaveraceae-type self-incompatibility system controlled by sS and pS.
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Keywords:
- Aconitum pendulum /
- RNA-seq /
- Self-incompatibility /
- S-RNase /
- SLF
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