Comparison of floral nectar extraction, preservation, and detection methods
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摘要: 花蜜富含多种有机物和微量元素，不同植物花蜜的分泌量以及化学物质构成存在很大差异，不同花蜜采集和保存方法都会影响其成分分析结果。本研究参考相关文献，总结了常用的6种野外花蜜收集方式（注射器法、毛细管法、滤纸法、溶液稀释法、离心法和抽吸法），6种花蜜保存方式（4 ℃冷藏保存、-20 ℃冷冻保存、-80 ℃超低温保存、-196 ℃液氮保存、添加防腐剂保存和滤纸保存）以及3类花蜜化学特征检测方式（手持折光仪、比色法和色谱分析）。深入比较了各种方法的优缺点及其潜在的适用对象，在条件允许的情况下，毛细管法、-80 ℃超低温保存及色谱分析是花蜜收集、保存及分析最常使用的方法。Abstract: Nectar is rich in organic compounds and microelements. However, nectar volume and chemical composition can vary substantially among plant species. Thus, nectar extraction and storage methods may affect component analysis. Based on a systematic review of the current literature, we summarizes six common nectar sampling methods in the field, including micropipettes and microsyringes, microcapillary tubes, filter paper, solution dilution, centrifugation, and aspiration; six nectar storage methods, including refrigeration at 4 ℃, freezing at -20 ℃, ultra-low freezing at -80 ℃, liquid nitrogen storage at -198 ℃, antimicrobial preservation, and filter paper storage; and three nectar characteristic measurement methods, including handheld refractometry, colorimetry, and chromatography. We further discuss the advantages and disadvantages of these methods and their potential application for different plant species. Under appropriate conditions, the most commonly used methods for nectar collection, storage, and analysis are microcapillary tubes, ultra-low freezing at -80 ℃, and chromatography.
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Keywords:
- Nectar /
- Nectar sampling /
- Nectar storage /
- Nectar measurement /
- Nectar composition
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[1] Anton S, Komon-Janczara E, Denisow B. Floral nectary, nectar production dynamics and chemical composition in five nocturnal Oenothera species (Onagraceae) in relation to floral visitors[J]. Planta, 2017, 246(6):1051-1067.
[2] Roy R, Schmitt AJ, Thomas JB, Carter CJ. Review:nectar biology:from molecules to ecosystems[J]. Plant Sci, 2017, 262:148-164.
[3] Park S, Thornburg RW. Biochemistry of nectar proteins[J]. J Plant Biol, 2009, 52:27-34.
[4] 卿卓, 苏睿, 董坤, 和绍禹. 花蜜化学成分及其生态功能研究进展[J]. 生态学杂志, 2014, 33(3):825-836. Qing Z, Su R, Dong K, He SY. Research progress on nectar compositions and their ecological functions[J]. Chinese Journal of Ecology, 2014, 33(3):825-836.
[5] Kearns CA, Inouye DW. Techniques for Pollination Biologists[M]. Colorado:University Press of Colorado, 1993:126-131.
[6] Parachnowitsch AL, Manson JS, Sletvold N. Evolutionary ecology of nectar[J]. Ann Bot, 2019, 123(2):247-261.
[7] Gijbels P, van den Ende W, Honnay O. Landscape scale variation in nectar amino acid and sugar composition in a Lepidoptera pollinated orchid species and its relation with fruit set[J]. J Ecol, 2014, 102(1):136-144.
[8] Kim YK, Lee S, Song JH, Kim MJ, Yunusbaev U, et al. Comparison of biochemical constituents and contents in floral nectar of Castanea spp.[J]. Molecules,2020, 25(18):4225.
[9] Chalcoff VR, Aizen MA, Galetto L. Nectar concentration and composition of 26 species from the temperate forest of South America[J]. Ann Bot, 2006, 97(3):413-421.
[10] Gillespie S, Elle E. Non-native plants affect generalist pollinator diet overlap and foraging behavior indirectly, via impacts on native plant abundance[J]. Biol Invasions, 2018, 20(11):3179-3191.
[11] Nepi M, von Aderkas P, Wagner R, Mugnaini S, Coulter A, et al. Nectar and pollination drops:how different are they?[J]. Ann Bot, 2009, 104(2):205-219.
[12] Johnson SD, Nicolson SW. Evolutionary associations between nectar properties and specificity in bird pollination systems[J]. Biol Lett, 2008, 4(1):49-52.
[13] Corbet SA. Nectar sugar content:estimating standing crop and secretion rate in the field[J]. Apidologie, 2003, 34(1):1-10.
[14] Bentley B, Elias T. The biology of nectaries[M]//Cruden RW, Hermann SM, eds. Studying Nectar? Some Observations on the Art. New York:Columbia University Press, 1983:223-241.
[15] McKenna MA, Thomson JD. Technique for sampling and measuring small amounts of floral nectar[J]. Ecology, 1988, 69(4):1306-1307.
[16] 李左栋, 刘静萱, 黄双全. 传粉生物学中几种花蜜采集和糖浓度测定方法的比较[J]. 植物分类学报, 2006, 44(3):320-326. Li ZD, Liu X, Huang SQ. A comparison of several methods used in the field for nectar collection and concentration analysis[J]. Acta Phytotaxonomica Sinica, 2006, 44(3):320-326.
[17] Mallick SA. Technique for washing nectar from flowers of Tasmanian leatherwood (Eucryphia lucida Eucryphia-ceae)[J]. Austral Ecol, 2000, 25(2):210-212.
[18] Swanson CA, Shuel RW. The centrifuge method for mea-suring nectar yield[J]. Plant Physiol, 1949, 25(3):513-520.
[19] 韩月鑫, 孙超, 张传利, 汪骞, 于丽欣, 等. 不同油菜品种花蜜化学成分及含量研究[J]. 云南农业大学学报(自然科学版), 2019, 34(4):571-575. Han YX, Sun C, Zhang CL, Wang Q, Yü LX, et al. Study on the chemical component and content of nectar from different rapeseed cultivars (Brassica napus L.)[J]. Journal of Yunnan Agricultural University (Natural Science), 2019, 34(4):571-575.
[20] Armstrong DP, Paton DC. Methods for measuring amounts of energy available from banksia inflorescences[J]. Aust J Ecol, 1990, 15(3):291-297.
[21] Nicolson SW, Nepi M, Pacini E. Nectaries and Nectar[M]. Netherlands:Springer, 2007.
[22] Morrant DS, Schumann R, Petit S. Field methods for sampling and storing nectar from flowers with low nectar vo-lumes[J]. Ann Bot, 2009, 103(3):533-542.
[23] 李婧, 肖秋生, 申济源, 陈厚彬. 荔枝花蜜分泌规律及可溶性糖组分和含量的分析[J]. 热带亚热带植物学, 2018, 26(5):490-496. Li J, Xiao QS, Shen JY, Chen HB. Analysis of secretion pattern and soluble sugar composition and contents in litchi nectar[J]. Journal of Tropical and Subtropical Botany, 2018, 26(5):490-496.
[24] Roguz K, Bajguz A, Chmur M, Golebiewska A, Roguz A, et al. Diversity of nectar amino acids in the Fritillaria (Liliaceae) genus:ecological and evolutionary implications[J]. Sci Rep, 2019, 9(3):191-200.
[25] Phillips RD, Bohman B, Brown GR, Tomlinson S, Peakall R. A specialised pollination system using nectar-seeking thynnine wasps in Caladenia nobilis (Orchidaceae)[J]. Plant Biol, 2019, 22(2):157-166.
[26] 孙立新, 胡红霞, 朱国强, 杨庆贵. 不同保存方法对蚊虫RNA保护效果的评价[J]. 中国国境卫生检疫杂志, 2011, 34(4):238-241. Sun LX, Hu HX, Zhu GQ, Yang QG. The evaluation of the protective effect of different preservation methods to mosquito RNA[J]. Chinese Frontier Health Quarantine, 2011, 34(4):238-241.
[27] 杨文贤, 刘亮, 朱富军, 吕礼芳, 詹球, 等. 液氮快速冻存法保存人羊膜抗冻剂筛选研究[J]. 华南国防医学杂志, 2013, 27(4):229-233. Yang WX, Liu L, Zhu FJ, Lü LF, Zhan Q, et al. Cryoprotectant screening to preserve human amnion by liquid nitrogen rapid cryopreserving[J]. Military Medical Journal of South China, 2013, 27(4):229-233.
[28] Seelenfreund E, Robinson WA, Amato CM, Tan AC, Kim J, et al. Long term storage of dry versus frozen RNA for next generation molecular studies[J]. PLoS One, 2014, 9(11):e111827.
[29] Witt T, Jurgens A, Gottsberger G. Nectar sugar composition of European Caryophylloideae (Caryophyllaceae) in relation to flower length, pollination biology and phylogeny[J]. J Evol Biol, 2013, 26(10):2244-2259.
[30] Jones CE, Little RJ. Handbook of pollination biology[M]//Baker HG, Baker I, eds. Floral Nectar Constituents in Relation to Pollinator Type. New York:Scientific and Academic Editions, 1983.
[31] Selisko B, Ulbrich R, Schellenberge A, Ute Müller. Inver-tase-catalyzed reactions in alcoholic solutions[J]. Biotechnol Bioeng, 1989, 35(10):1006-1010.
[32] Rodriguez M, Gomez A, Gonzalez F, Barzana E, Lopez-Munguia A. Stability of invertase in alcoholysis reactions with methanol[J]. J Mol Catal B:Enzym, 1997, 2(6):299-306.
[33] Canto A, Herrera CM. Micro-organisms behind the pollination scenes:microbial imprint on floral nectar sugar variation in a tropical plant community[J]. Ann Bot, 2012, 110(6):1173-1183.
[34] Galetto L, Bernardello G. Floral nectaries, nectar production dynamics and chemical composition in six Ipomoea species (Convolvulaceae) in relation to pollinators[J]. Ann Bot, 2004, 94(2):269-280.
[35] 孙兰, 黎淑端, 聂木海, 廖育煌, 李秀珍. 一起食品中还原糖测定结果异常的原因分析[J]. 中国卫生检验杂志, 2005, 15(9):1137. [36] 中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局.食品安全国家标准:食品中果糖、葡萄糖、蔗糖、麦芽糖、乳糖的测定:GB5009.8-2016[S]. 北京:中国标准出版社, 2016. [37] 丁洪流, 李灿, 金萍, 袁丽红, 姚永青, 等. 高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇[J]. 色谱, 2003, 31(8):804-808. Ding HL, Li C, Jin P, Yuan LJ, Yao YQ, et al. Simulta-neous determination of monosaccharides, disaccharides, oligosaccharides and sugar alcohols in foods by high performance liquid chromatography with evaporative light-scattering detection[J]. Chinese Journal of Chromatography, 2003, 31(8):804-808.
[38] Sun SG, Huang ZH, Chen ZB, Huang SQ. Nectar properties and the role of sunbirds as pollinators of the golden-flowered tea (Camellia petelotii)[J]. Am J Bot, 2017, 104(3):468-476.
[39] 丁永胜, 牟世芬. 氨基酸直接分析法原理及应用[J]. 现代仪器, 2002, (1):11-14. Ding YS, Mou SF. The principle and application of amino acids analysis direct system[J]. Modern instruments, 2002, (1):11-14.
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