两种红树植物不同种源幼苗对模拟高温热浪的光合生理响应差异

张小燕; Alison Kim Shan Wee; Tadashi Kajita; 朱俊杰; 曹坤芳

doi:10.11913/PSJ.2095-0837.2021.40424

两种红树植物不同种源幼苗对模拟高温热浪的光合生理响应差异

1. 广西大学亚热带农业生物资源保护与利用国家重点实验室, 南宁 530004;
2. 广西大学林学院, 广西森林生态与保育重点实验室, 南宁 530004;
3. 琉球大学热带生物研究中心, 西表研究站, 日本冲绳县 9071541

基金项目:

国家自然科学基金（31670406）；八桂学者人才项目（33600992001）。

详细信息

作者简介:
张小燕(1996-),女,硕士研究生,研究方向为植物生理生态(E-mail:1834151176@qq.com)。

通讯作者:
曹坤芳,E-mail:kunfangcao@gxu.edu.cn

中图分类号: Q945
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出版历程
- 收稿日期: 2020-12-21
- 修回日期: 2021-02-12
- 网络出版日期: 2022-10-31
- 发布日期: 2021-08-27

Differences in photosynthetic responses to simulated heat wave in seedlings of two mangrove species from different provenances

1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China;
2. Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, China;
3. Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, Japan 9071541

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31670406) and Bagui Scholar Talent Project (33600992001)。

摘要

摘要: 以来自中国广西（较低纬度）和日本冲绳（较高纬度）两个地理种群的耐冷秋茄（Kandelia obovata Sheue，H.Y.Liu&J.Yong）和耐热木榄（Bruguiera gymnorrhiza （L.） Savigny）为材料，测定其光系统Ⅰ（PSⅠ）和光系统Ⅱ（PSⅡ）活性参数、叶黄素循环组分和叶绿素a、b的含量，并分析他们经高温处理后光合生理响应的差异。结果显示：高温处理使植物PSⅡ最大光化学潜能（F_v/F_m）显著降低，降低幅度为较低纬度种群<较高纬度种群、木榄幼苗<秋茄幼苗；P700氧化还原状态（P_m）降低，降低幅度为较低纬度种群<较高纬度种群；叶片非光化学猝灭（NPQ）升高，表现为较低纬度种群>较高纬度种群、木榄幼苗>秋茄幼苗。高温处理引起叶黄素库容量（V+A+Z）和脱环氧化状态（A+Z）/（V+A+Z）升高，表现为较低纬度种群>较高纬度种群；（A+Z）/（V+A+Z）与F_v/F_m负相关，而与NPQ正相关。常温恢复第2 d，高纬度种源秋茄幼苗F_v/F_m仍为0.69，表明其PSⅡ受到不可逆的损伤，而其余种源幼苗的F_v/F_m均恢复到正常水平，且所有种源幼苗仍保持较高的NPQ以及叶黄素脱环氧化比率，起光保护作用。与萨瓦纳和地中海型生境相比，红树林有最大的叶黄素库。研究结果表明，低纬度种群对高温胁迫具有较高的适应性，部分原因是叶黄素循环具有较强的光保护作用，物种及种源间存在耐冷和耐热性的权衡。
- 红树植物 /
- 光合系统 /
- 高温胁迫 /
- 叶黄素循环 /
- 种源差异
Abstract: We analyzed the impact of two days of high-temperature treatment on the leaf photosystem properties of potted seedlings of two mangrove species, i.e., cold-tolerant Kandelia obovata Sheue, H. Y. Liu & J. Yong and heat-tolerant Bruguiera gymnorrhiza (L.) Savigny, from two geographical populations in Guangxi, China (lower latitude) and Okinawa, Japan (higher latitude). The xanthophyll cycle components and chlorophyll-a and -b contents were measured. The leaf photosystemⅠ(PSⅠ) and photosystemⅡ(PSⅡ) activities were measured using a Dual-PAM-100 system. Results showed that high temperature significantly reduced the maximum photochemical efficiency (F_v/F_m) of PSⅡ in both species. The F_v/F_m values showed less decrease in the lower latitude populations than in the higher latitude populations, and less decrease in B. gymnorrhiza than in K. obovata seedlings. The P700 oxidation-reduction state (P_m) showed less decrease in the lower than higher latitude populations. The leaf non-photochemical quenching (NPQ) showed a greater increase in the lower latitude populations than in the higher latitude populations. The xanthophyll pool size (V + A + Z) and de-epoxidation ratio (A + Z)/(V + A + Z) increased following heat treatment. A greater increase was observed in the lower latitude populations than in the higher latitude populations. In addition, the (A + Z)/(V + A + Z) ratio was negatively correlated with F_v/F_m, but positively correlated with NPQ. Compared with other hardy habitats, such as savannas and Mediterranean woodlands, mangroves had the largest xanthophyll pool size. On the second day of recovery, F_v/F_m was still at 0.69 in the high-latitude K. obovata seedlings, indicating irreversible photoinhibition of PSⅡ, while all seedlings maintained a higher de-epoxidation ratio and NPQ for photosystem protection. In conclusion, the lower latitude populations showed higher adaptability to heat stress, which was partly due to strong photoprotection through the xanthophyll cycle, and there was a tradeoff between cold- and heat-tolerance between species and populations. This study revealed a novel heat tolerance mechanism in mangrove species, which has implications for their physiological responses to future changing climate.
- Mangrove /
- Photosystems /
- High temperature stress /
- Xanthophyll cycle /
- Provenance

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