Genome-wide identification of pepper OSCA gene family and expression analysis under different stress conditions
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摘要: 钙通透性阳离子通道蛋白（OSCA）在渗透胁迫感应中发挥重要的作用。本研究利用辣椒（Capsicum annuum L.）全基因组信息，鉴定出14个OSCA基因（CaOSCA），染色体定位及同源性分析表明，它们分别位于1、2、4、6、7、8、9、12号染色体上，亚细胞定位预测表明其编码产物均位于质膜上。系统进化树分析这些基因可分为4个组，分别命名为Ⅰ~Ⅳ，与拟南芥存在11对共线性基因。顺式作用元件预测显示CaOSCA基因能响应激素和非生物胁迫，表达分析显示多数CaOSCA基因能响应干旱、盐和低温胁迫。干旱胁迫24 h时，CaOSCA8和CaOSCA11相对表达量显著高于对照，分别上升14和13倍。Abstract: The calcium-permeable cation channel protein (OSCA) plays an important role in osmotic stress response. In this study, 14 OSCA family members were identified using whole-genome information of pepper (Capsicum annuum L.). Chromosomal localization and homology analysis showed that the OSCA family members were located on chromosomes 1, 2, 4, 6, 7, 8, 9, and 12, respectively. Subcellular localization prediction showed that they were all located on the plasma membrane. Phylogenetic tree analysis indicated that the CaOSCA gene family could be divided into four subfamilies, named Ⅰ-Ⅳ, and there were 11 collinear gene pairs with Arabidopsis. Cis-acting element prediction showed that the CaOSCA family genes may respond to hormone and abiotic stress. Furthermore, relative expression analysis of pepper showed that most CaOSCA genes respond to drought, salt, and low temperature stresses. Under drought stress for 24 h, the relative expression levels of CaOSCA8 and CaOSCA11 were significantly higher than those in the control, increasing by 14 and 13 times, respectively. This study provides new information for the OSCA gene family and provides a reference for subsequent study of OSCA in pepper.
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Keywords:
- Capsicum annuum /
- OSCA /
- Family analysis /
- Abiotic stress
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