葡萄Golden2-like转录因子家族的鉴定及其表达分析

许晨; 李青云; 万俊男; 侯雨君; 周慧敏; 朱振飞; 辛海平; 李吉涛

doi:10.11913/PSJ.2095-0837.2022.20205

葡萄Golden2-like转录因子家族的鉴定及其表达分析

许晨^1,2,3,
李青云^2,3,
万俊男^2,3,
侯雨君^2,3,
周慧敏^2,3,
朱振飞^2,3,
辛海平^2,3,
李吉涛^1, ,

1. 湖北民族大学林学园艺学院, 湖北恩施 445000;
2. 中国科学院武汉植物园, 中国科学院植物种植创新与特色农业重点实验室, 武汉 430074;
3. 中国科学院中-非联合研究中心, 武汉 430074

基金项目:

湖北省自然科学基金（2021CFB555）；湖北民族大学博士启动基金（MD2019B016）。

详细信息

作者简介:
许晨(1996-),女,硕士研究生,研究方向为葡萄抗逆机理(E-mail:2287183284@qq.com)。

通讯作者:
李吉涛,E-mail:ljtyouth@foxmail.com

中图分类号: Q943.2
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出版历程
- 收稿日期: 2021-09-07
- 修回日期: 2021-10-18
- 网络出版日期: 2022-10-31
- 发布日期: 2022-04-27

Identification and expression analysis of the Golden2-like transcription factor family of Vitis vinifera L.

Xu Chen^1,2,3,
Li Qinq-Yun^2,3,
Wan Jun-Nan^2,3,
Hou Yu-Jun^2,3,
Zhou Hui-Min^2,3,
Zhu Zhen-Fei^2,3,
Xin Hai-Ping^2,3,
Li Ji-Tao^1, ,

1. School of Forestry and Horticulture, Hubei Minzu University, Enshi, Hubei, 445000;
2. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
3. Sino-Africa Joint research Center, Chinese Academy of Sciences, Wuhan 430074, China

Funds:

This work was supported by grants from the Natural Science Foundation of Hubei Province (2021CFB555) and Doctoral Startup Founding of Hubei Minzu University (MD2019B016).

摘要

摘要: 本研究对葡萄（Vitis vinifera L.）的Golden2-like （GLK）转录因子家族进行了全基因组鉴定和表达模式分析，并利用品种‘玫瑰香’（V.vinifera cv.Muscat Hamburg）进一步验证其在低温胁迫下的响应。结果显示，葡萄Golden2-like家族共46个成员，分为5个亚族，同一亚族的保守结构域相似。46个VvGLK分别定位于细胞核、叶绿体、细胞质和过氧化物酶体中，其启动子区域含多种逆境应答顺式作用元件。基因芯片分析结果表明，22个Golden2-like基因在果实发育过程中变化显著。同时，有15、15和9个基因分别响应盐、干旱和低温胁迫。qRT-PCR分析发现26个基因参与低温应答。VvGLK41在所有胁迫处理中均下调表达。
- 葡萄 /
- Golden2-like /
- 低温胁迫 /
- 果实发育 /
- 表达分析
Abstract: In this study, the Golden2-like transcription factor (TF) gene family was identified and analyzed in Vitis vinifera L. We further verified the expression patterns of Golden2-like genes under low temperature in V. vinifera ‘Muscat Hamburg’ seedlings. Results showed that 46 Golden2-like genes were identified in V. vinifera and classified into five subgroups. The conserved domain was similar within the same subgroup. Forty-six VvGLKs were located in the nucleus, chloroplast, cytoplasm, and peroxisome, and their promoter regions contained a variety of cis-elements related to stress response. Gene chip data showed that 22 VvGLKs changed significantly during fruit development and 15, 15, and 9 VvGLKs were expressed after salt, drought, and low-temperature stress, respectively. qRT-PCR identified 26 VvGLKs involved in cold-stress response. Notably, VvGLK41 was down-regulated after salt, drought, and low-temperature treatment. Thus, this study provides clues for fruit development and stress response in grape.
- Vitis vinifera /
- Golden2-like gene /
- Low-temperature stress /
- Fruit development /
- Expression analysis

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