强德勒红心柚离体培养与植株再生体系的建立

详细信息

Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv.Chandler

1.
College of Life Science,China West Normal University, Nanchong, Sichuan 637002, China
2.
Sichuan Provincial Key Laboratory of Ecology and Environmental Technology, Mianyang, Sichuan 621000, China
3.
Fruit Research Institute,Chongqing Academy of Agricultural Science, Jiangjin, Chongqing 402260, China

摘要: 以强德勒红心柚(Citrus grandis Osbeck cv.Chandler)种子萌发的无菌苗为材料,选取子叶、子叶节段、上胚轴、带芽的茎段进行离体培养研究.结果表明:子叶节段是诱导丛生芽的最佳外植体,诱导率100%;诱导丛生芽的最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+蔗糖30 g/L+活性炭0.4 g/L,丛生芽增殖可达11.2倍;最适生根培养基为1/2MS+NAA 0.5 mg/L,生根率达100%,移栽15 d后成活率100%.
- 强德勒红心柚 /
- 离体培养 /
- 丛生芽增殖
Abstract: The cotyledons,cotyledonary nodes,epicotyl segments,and stem segments with lateral buds of aseptic Citrus grandis Osbeck cv.Chandler seedings were used as the explants to study the process of tissue culture rapid reproduction.Results showed that the cotyledonary nodes were the best explants of bush-bud induction(induction rate 100%).The best medium for inducing bush-bud was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+sucrose 30 g/L+activated carbon 0.4 g/L,the coefficient of proliferation reached 11.2.The optimum medium for shoot rooting was 1/2MS+NAA 0.5 mg/L,which resulted in a rooting rate of 100%.Fifteen days after transplanting,100% of the plantlets survived.
- Citrus grandis Osbeck cv.Chandler /
- Culture in vitro /
- Bush-bud regeneration