赤霉素、钙和甜菜碱对小桐子种子萌发及幼苗抗低温和干旱的影响

代勋; 李忠光; 龚明

doi:10.3724/SP.J.1142.2012.20204

赤霉素、钙和甜菜碱对小桐子种子萌发及幼苗抗低温和干旱的影响

代勋^1,2, ,,
李忠光¹,
龚明¹

1.
云南师范大学生命科学学院,生物能源持续开发利用教育部工程研究中心, 云南省生物质能与环境生物技术重点实验室, 昆明 650092
2. 昭通师范高等专科学校, 云南昭通 657000

基金项目: 云南省教育厅科研基金重大专项项目(ZD2010004);云南省自然科学基金(2010ZC066)。

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通讯作者:
代勋, E-mail: gongming63@163.com

中图分类号: Q945.78
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- 收稿日期: 2011-05-27
- 修回日期: 2011-11-30
- 发布日期: 2012-04-29

Effect of Gibberellin, Calcium,and Betaine on Seed Germination and Resistance of Jatropha curcas L.Seedlings to Low Temperature and Drought Stress

1.
School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology, Yunnan Province, Yunnan Normal University, Kunming 650092, China
2. Zhaotong Teacher’s College, Zhaotong, Yunnan 657000, China

摘要

摘要: 小桐子(Jatropha curcas L.)属大戟科( Euphorbiaceae)麻疯树属(Jatropha L.)的能源植物。分别以小桐子种子和幼苗为实验材料,研究了不同浓度的赤霉素和CaCl₂单独处理,以及不同浓度的赤霉素、CaCl₂和甜菜碱组合处理对小桐子种子萌发及幼苗抗低温和干旱的影响。结果表明:分别用10 mg/L赤霉素和10 mmol/L CaCl₂处理小桐子种子,不仅可以提高其在正常萌发条件(26℃)、低温(18℃)和干旱胁迫(5% PEG6000)下的发芽率,还可缓解小桐子幼苗在低温(2℃)或干旱胁迫(25% PEG6000)下电解质渗漏率的增加和丙二醛(MDA)的积累。用10 mg/L赤霉素、5 mmol/L CaCl₂和15 mmol/L甜菜碱组合处理小桐子种子,也可进一步增强其在正常萌发(26℃)、低温(18℃)和干旱胁迫(5% PEG6000)条件下种子的发芽率,以及缓解小桐子幼苗在低温(2℃)或干旱胁迫(25% PEG6000)下电解质渗漏率的增加和MDA的积累,表明10 mg/L赤霉素和10 mmol/L CaCl₂分别处理或10 mg/L赤霉素、5 mmol/L CaCl₂和10 mmol/L甜菜碱组合处理可提高小桐子种子在低温和干旱胁迫下的发芽率,以及提高小桐子幼苗对低温和干旱胁迫的抵抗能力。
- 小桐子 /
- 赤霉素 /
- 钙 /
- 甜菜碱 /
- 低温抗性 /
- 抗旱性
Abstract: Jatropha curcas L.is an energy plant of the Euphorbiaceae family.Using seeds and seedlings of J.curcas as materials,the effect of different concentrations of gibberellin or calcium treatment,or combination treatment of different concentrations of gibberellin,calcium, and betaine on seed germination and resistance of seedlings to low temperature or drought stress were investigated.The application of 10 mg/L gibberellin or 10 mmol/L calcium not only improved germination percentage of J.curcas seeds under normal conditions (26℃),low temperature (18℃),and drought stress (5% PEG6000),but also alleviated electrolyte leakage and accumulation of MDA in J.curcas seedlings under low temperature (2℃) and drought stress (25% PEG6000) compared with the control. In addition,combination treatments with 10 mg/L gibberellin,5 mmol/L calcium,and 15 mmol/L betaine also enhanced germination percentage of J.curcas seeds under normal conditions (26℃),low temperature (18℃),and drought stress (5% PEG6000),and alleviated electrolyte leakage and accumulation of MDA in J.curcas seedlings under low temperature (2℃) and drought stress (25% PEG6000),illustrating that treatment with 10 mg/L gibberellin or 10 mmol/L calcium,or combination treatments of 10 mg/L gibberellin,5 mmol/L calcium,and 15 mmol/L betaine increased germination percentage of J.curcas seeds under low temperature and drought stress,and resistance of seedlings to low temperature or drought stress.
- Jatropha curcas L. /
- Gibberellin(GA) /
- Calcium /
- Betaine /
- Low temperature resistance /
- Drought resistance

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参考文献(37)

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赤霉素、钙和甜菜碱对小桐子种子萌发及幼苗抗低温和干旱的影响

通讯作者: 代勋, E-mail: gongming63@163.com

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Effect of Gibberellin, Calcium,and Betaine on Seed Germination and Resistance of Jatropha curcas L.Seedlings to Low Temperature and Drought Stress

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通讯作者:
代勋, E-mail: gongming63@163.com