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千里光热激蛋白90-3(Hsp90-3)的生物信息学与功能分析

平军娇, 张珍, 蔡振锋, 汤贤春, 钱刚

平军娇, 张珍, 蔡振锋, 汤贤春, 钱刚. 千里光热激蛋白90-3(Hsp90-3)的生物信息学与功能分析[J]. 植物科学学报, 2012, 30(4): 385-393. DOI: 10.3724/SP.J.1142.2012.40385
引用本文: 平军娇, 张珍, 蔡振锋, 汤贤春, 钱刚. 千里光热激蛋白90-3(Hsp90-3)的生物信息学与功能分析[J]. 植物科学学报, 2012, 30(4): 385-393. DOI: 10.3724/SP.J.1142.2012.40385
PING Jun-Jiao, ZHANG Zhen, CAI Zhen-Feng, TANG Xian-Chun, QIAN Gang. Functional Roles of Heat Shock Proteins 90-3(Hsp90-3) in Senecio scandens Buch.-Ham.ex D.Don Based on Its Bioinformatics[J]. Plant Science Journal, 2012, 30(4): 385-393. DOI: 10.3724/SP.J.1142.2012.40385
Citation: PING Jun-Jiao, ZHANG Zhen, CAI Zhen-Feng, TANG Xian-Chun, QIAN Gang. Functional Roles of Heat Shock Proteins 90-3(Hsp90-3) in Senecio scandens Buch.-Ham.ex D.Don Based on Its Bioinformatics[J]. Plant Science Journal, 2012, 30(4): 385-393. DOI: 10.3724/SP.J.1142.2012.40385
平军娇, 张珍, 蔡振锋, 汤贤春, 钱刚. 千里光热激蛋白90-3(Hsp90-3)的生物信息学与功能分析[J]. 植物科学学报, 2012, 30(4): 385-393. CSTR: 32231.14.SP.J.1142.2012.40385
引用本文: 平军娇, 张珍, 蔡振锋, 汤贤春, 钱刚. 千里光热激蛋白90-3(Hsp90-3)的生物信息学与功能分析[J]. 植物科学学报, 2012, 30(4): 385-393. CSTR: 32231.14.SP.J.1142.2012.40385
PING Jun-Jiao, ZHANG Zhen, CAI Zhen-Feng, TANG Xian-Chun, QIAN Gang. Functional Roles of Heat Shock Proteins 90-3(Hsp90-3) in Senecio scandens Buch.-Ham.ex D.Don Based on Its Bioinformatics[J]. Plant Science Journal, 2012, 30(4): 385-393. CSTR: 32231.14.SP.J.1142.2012.40385
Citation: PING Jun-Jiao, ZHANG Zhen, CAI Zhen-Feng, TANG Xian-Chun, QIAN Gang. Functional Roles of Heat Shock Proteins 90-3(Hsp90-3) in Senecio scandens Buch.-Ham.ex D.Don Based on Its Bioinformatics[J]. Plant Science Journal, 2012, 30(4): 385-393. CSTR: 32231.14.SP.J.1142.2012.40385

千里光热激蛋白90-3(Hsp90-3)的生物信息学与功能分析

基金项目: 贵州省优秀科技教育人才省长基金项目(黔省专合字2008-61号)。
详细信息
    通讯作者:

    钱刚, E-mail: pengjiaqiong@163.com

  • 中图分类号: Q7; Q943

Functional Roles of Heat Shock Proteins 90-3(Hsp90-3) in Senecio scandens Buch.-Ham.ex D.Don Based on Its Bioinformatics

  • 摘要: Hsp90是真核细胞重要的一类分子伴侣,与植物的生长发育、抗逆性、信号转导及生物进化等功能密切相关。为了深入理解高等植物Hsp90结构与功能的关系,该研究从千里光(Senecio scandens)全长cDNA文库中分离到Hsp90-3基因。序列分析结果表明,该基因编码699个氨基酸的多肽,与拟南芥(Arabidopsis thaliana)AtHsp90-3(登录号:NP_200412.1)的同源性最高,为93.71%;预测蛋白质的分子量为79.78 kD,理论等电点为5.08。信号序列分析结果发现,该蛋白主要定位于细胞的细胞核、过氧化物酶体、叶绿体类囊体膜及叶绿体基质中,提示作为分子伴侣,高等植物Hsp90-3参与细胞内膜系统蛋白质的转运。结构与功能分析发现,该蛋白有3个结构域及1个连接区,推测Hsp90-3在真核细胞的信号转导、转录调控及胁迫表达等过程中发挥重要功能。
    Abstract: Heat shock proteins (Hsp),representing an important molecular chaperone in eukaryotic cells,is a common response to development,stress resistance,signal transduction and evolution of plants.The relationship between the structure and functional roles was elucidated in Hsp90 based on the generation of full-length cDNAs from Senecio scandens Buch.-Ham.ex D.Don.Sequence analysis of Hsp90-3 gene indicated that it shared 93.71% identity with Arabidopsis thaliana (GenBank accession: NP_200412.1),encoding a protein composed of 699 amino acid residues with the predicted molecular weight of 79.78 kD and theoretical isoelectric point of 5.08.Moreover,the distribution of Hsp90-3 was involved in the endomembrane system such as nuclei,peroxisomes,chloroplast thylakoid membranes,and chloroplast matrices in the present study.Three-dimensional measurement revealed that the Hsp90-3 protein was composed of three structural domains and one link region.These results suggested that Hsp90-3 played a critical role in molecular chaperone,signal transduction,transcriptional regulation and stress-response in higher plants.
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出版历程
  • 收稿日期:  2012-02-23
  • 修回日期:  2012-06-26
  • 发布日期:  2012-08-29

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