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大白菜与紫甘蓝种间杂种的获得与鉴定

乔海云, 李菲, 张淑江, 章时蕃, 张慧, 孙日飞

乔海云, 李菲, 张淑江, 章时蕃, 张慧, 孙日飞. 大白菜与紫甘蓝种间杂种的获得与鉴定[J]. 植物科学学报, 2012, 30(4): 407-414. DOI: 10.3724/SP.J.1142.2012.40407
引用本文: 乔海云, 李菲, 张淑江, 章时蕃, 张慧, 孙日飞. 大白菜与紫甘蓝种间杂种的获得与鉴定[J]. 植物科学学报, 2012, 30(4): 407-414. DOI: 10.3724/SP.J.1142.2012.40407
shu
QIAO Hai-Yun, LI Fei, ZHANG Shu-Jiang, ZHANG Shi-Fan, ZHANG Hui, SUN Ri-Fei. Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage[J]. Plant Science Journal, 2012, 30(4): 407-414. DOI: 10.3724/SP.J.1142.2012.40407
Citation: QIAO Hai-Yun, LI Fei, ZHANG Shu-Jiang, ZHANG Shi-Fan, ZHANG Hui, SUN Ri-Fei. Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage[J]. Plant Science Journal, 2012, 30(4): 407-414. DOI: 10.3724/SP.J.1142.2012.40407
shu
乔海云, 李菲, 张淑江, 章时蕃, 张慧, 孙日飞. 大白菜与紫甘蓝种间杂种的获得与鉴定[J]. 植物科学学报, 2012, 30(4): 407-414. CSTR: 32231.14.SP.J.1142.2012.40407
引用本文: 乔海云, 李菲, 张淑江, 章时蕃, 张慧, 孙日飞. 大白菜与紫甘蓝种间杂种的获得与鉴定[J]. 植物科学学报, 2012, 30(4): 407-414. CSTR: 32231.14.SP.J.1142.2012.40407
shu
QIAO Hai-Yun, LI Fei, ZHANG Shu-Jiang, ZHANG Shi-Fan, ZHANG Hui, SUN Ri-Fei. Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage[J]. Plant Science Journal, 2012, 30(4): 407-414. CSTR: 32231.14.SP.J.1142.2012.40407
Citation: QIAO Hai-Yun, LI Fei, ZHANG Shu-Jiang, ZHANG Shi-Fan, ZHANG Hui, SUN Ri-Fei. Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage[J]. Plant Science Journal, 2012, 30(4): 407-414. CSTR: 32231.14.SP.J.1142.2012.40407
shu

大白菜与紫甘蓝种间杂种的获得与鉴定

  • 中国农业科学院蔬菜花卉研究所, 北京 100081

基金项目: 现代农业产业技术体系建设专项(CARS-25-A-01);中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项(2060302-02-12);农业部园艺作物生物学与种质创制重点实验室开放基金。
详细信息
    通讯作者:

    孙日飞, E-mail: rifei.sun@caas.net.cn

  • 中图分类号: Q943; S634

Production and Identification of Interspecific Hybrids between Chinese Cabbage and Purple Cabbage

  • Institute of Vegetable and Flowers,Chinese Academy of Agricultural Sciences, Beijing 100081, China

摘要

    摘要
  • 摘要: 以不同类型的大白菜与紫甘蓝为材料,运用蕾期授粉结合胚挽救技术获得大白菜与紫甘蓝的种间杂种,并对其进行细胞学鉴定。结果表明,大白菜与紫甘蓝杂交,获得了57株F1代幼苗;对根尖染色体数量进行观察和杂种花粉特性调查发现,其中47株具有预期的染色体数目,2n=19,鉴定为真杂种,其花粉败育;另有6株具有38条染色体,鉴定为种间异源双二倍体,应该是发生了染色体自然加倍,其花粉可育。可育的杂种F1代与大白菜回交,获得了大白菜—紫甘蓝BC1代材料。田间观测结果显示,杂种F1代植株综合性状均介于双亲之间,BC1代植株包球明显,综合性状偏向母本大白菜。
    Abstract: Interspecific hybridizations between different varieties of Brassica rapa L.ssp.peki-nensis (Lour.) Olsson and Brassica oleracea var.capitata L.were carried out. The hybrids were obtained by successive bud pollination and ovule culture and tested by means of identification by cytology.Results showed that 57 plants were obtained by interspecific hybridization between B.rapa L.ssp.pekinensis and B.oleracea var.capitata L.Cytological identification and pollen characteristics survey revealed that 47 of the hybrids had the anticipated chromosomes (2n=19) and pollen fertility was aborted; while 6 hybrids had 38chromosomes and were fertile.The BC1 plants were obtained through back cross between fertile hybrids F1 and B.rapa L.ssp.pekinensis.Field investigation showed that all F1 hybrids had many characteristics from both parents,the BC1 plants had obvious heading and were similar to B.rapa L.ssp.pekinensis.
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    • 参考文献(26)
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出版历程
  • 收稿日期:  2012-02-14
  • 修回日期:  2012-04-16
  • 发布日期:  2012-08-29

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