滑菇多糖wPNP-a1的制备及结构分析

郭国赟; 孙润广; 郭琦; 张小飞; 窦佩娟

doi:10.3724/SP.J.1142.2012.40423

滑菇多糖wPNP-a1的制备及结构分析

陕西师范大学物理学与信息技术学院,生物物理与生物医学工程实验室, 西安 710062

基金项目: 国家自然科学基金资助项目(10874108);陕西省自然科学基础研究计划(SJ08A16)。

详细信息

通讯作者:
孙润广, E-mail: sunrunguang@snnu.edu.cn

中图分类号: Q946.3
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出版历程
- 收稿日期: 2011-11-02
- 修回日期: 2012-02-18
- 发布日期: 2012-08-29

Preparation and Structural Characterization of wPNP-a1 from Pholiota nameko

Laboratory of Biophysics and Biomedical Technology, College of Physics and Information Technology, Shaanxi Normal University, Xi’an 710062, China

摘要

摘要: 利用传统热水浸提法提取滑菇粗多糖(wPNP),进而用季铵盐沉淀法和Sephadex G-150凝胶色谱柱层析对wPNP进行纯化,得滑菇多糖wPNP-a1。以高效液相色谱(HPLC)、傅里叶变换红外光谱(FTIR)、气相色谱(GC)、核磁共振(NMR)、环境扫描电子显微镜(ESEM)和原子力显微镜(AMF)等方法分析wPNP-a1的结构,结果表明,滑菇多糖wPNP-a1的分子量为419310 Da,具有一般类多糖的特征吸收峰,糖苷键为β-型,不含乙酰基,由木糖、甘露糖、半乳糖和葡萄糖组成,其摩尔比为3.91∶2.77∶1.91∶1;wPNP-a1的分子间排斥力较大,分子间吸引力较小,在云母表面呈直链状分布,链宽23 nm左右,链高0.7~0.8 nm。
- 滑菇 /
- 滑菇多糖 /
- 热水提取 /
- 结构
Abstract: Water-soluble crude polysaccharide (wPNP) was extracted from cultured mycelium of the fungus Pholiota nameko by hot water extraction.The extraction rates of polysaccharide were 2.463%.The content of total polysaccharide was determined by the phenol-sulphuric acid method,and the value was 64.98%.The crude polysaccharides were purified by CTAB and Sephadex-150 in sequence,with the obtained one designated as wPNP-a1.The structure of wPNP-a1 was analysed by HPLC,FTIR,GC,NMR,ESEM,and AFM.The results showed that the average molecular weight of wPNP-a1 was 419310 Da.It had the general characteristics of polysaccharide absorption peaks,β-glycoside linkage,and no acetyl.The GC analysis indicated wPNP-a1 was composed of xylose,mannose,galactose,and glucose in molar ratios of 3.91∶2.77∶1.91∶1.The repelling forces between the molecules were stronger than the attractive forces. The wPNP-a1 formed a linear chain on the surface of mica,the width was 23 nm,and the height was 0.7-0.8 nm.
- Pholiota nameko /
- Pholiota nameko polysaccharides /
- Hot water extraction /
- Structure characterization

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参考文献(18)

[1]	倪福太,庄妍.植物多糖研究进展[J].牡丹江师范学院学报, 2010, 4: 34-36.
[2]	钟昕,周素梅,王强.药用真菌多糖研究进展[J].科技导报, 2009, 27(9): 97-101.
[3]	杜庆.食(药)用真菌多糖的研究进展[J].中国食物与营养, 2011, 17(5): 75-77.
[4]	于淑池,侯金鑫,李明艳,李鹏程,陈爱萍.滑菇多糖的超声波辅助提取工艺及抗氧化活性研究[J].食品科学, 2010, 31(4): 59-62.
[5]	李海平,张树海,张坤生.滑菇多糖抗氧化活性研究[J].食品研究与开发, 2008, 29(4): 56-60.
[6]	崔英俊,李庆章.滑菇多糖对衰老模型鼠不同时期免疫功能的影响[J].东北农业大学学报, 2004, 35(2): 129-134.
[7]	Li H P,Wang S.Kinetics of inhibition of ribonuclease A by Pholiota nameko polysaccharide[J].Int J Biol Macromol, 2007, 40: 134-138.
[8]	舒任庚,蒋跃平,蔡永红.植物多糖的提取分离方法探讨[J].中国药房, 2011, 22(11): 1052-1055.
[9]	陈欣,龚兰,刘冠卉.食用真菌多糖提取条件的优化及其还原力的比较[J].食品科学, 2010, 31(14): 140-144.
[10]	Cinthia B S Telles,Diego A Sabry,Jailma Almeida-Lima,Mariana S S P Costa,et al.Sulfation of the extracellular polysaccharide produced by the edible mushroom Pleurotus sajorcaju alters its antioxidant,anticoagulant and antiproliferative properties in vitro[J].Carbohydr Polym, 2011, 85: 514-521.
[11]	Yang Bao,Jiang Yueming,Zhao Mouming,et al.Structural characterisation of polysaccharides purified from longan (Dimocarpus longan Lour.) fruit pericarp[J].Food Chem, 2009, 115: 609-614.
[12]	Eliza Malinowska,Wojciech Krzyczkowski,Grzegorz Lapienis,Franciszed Herold.Densitometric determination of carbohydrates: Application to puridication and molecular weight determination of polysaccharide from Hericium erinaceum mushroom[J].Food Res Int, 2010, 43: 988-995.
[13]	Wang J L,Guo H Y,Zhang J,et al.Sulfated modification,characterization and structure-antioxidant relationships of Artemisia sphaerocephala polysaccharides[J].Carbohydr Polym, 2010, 81: 897-905.
[14]	Qian J Y,Chen W,Zhang W M,Zhang H.Adulteration identification of some fungal polysaccharides with SEM,XRD,IR and optical rotation: A primary approach[J].Carbohydr Polym, 2009, 78: 620-625.
[15]	Qiao D L,Liu J,Ke C L,et al.Structural characte-rization of polysaccharides from Hyriopsis cumingii[J].Carbohydr Polym, 2010, 82: 1184-1190.
[16]	胡艳南.海萝藻(Gloiopeltis furcata)多糖的提取分离及其结构研究.青岛: 中国海洋大学, 2010.
[17]	Yan H,Xie Y P,Sun S G,et al.Chemical analysis of Astragalus mongholicus polysaccharides and antioxidant activity of the polysaccharides[J].Carbohydr Polym, 2010, 82: 636-640.
[18]	Feng T,Gu Z B,Jin Z Y,Zhuang H N.Isolation and characterization of an acidic polysaccharide from Mesona blumes gum[J].Carbohydr Polym, 2008, 71: 159-169.

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滑菇多糖wPNP-a1的制备及结构分析

通讯作者: 孙润广, E-mail: sunrunguang@snnu.edu.cn

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Preparation and Structural Characterization of wPNP-a1 from Pholiota nameko

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通讯作者:
孙润广, E-mail: sunrunguang@snnu.edu.cn