拟南芥AtJ3通过核质转运调控植物质膜H<sup>+</sup>-ATPase活性与ABA响应

赵菲佚; 焦成瑾; 贾贞; 周辉; 王茂; 赵雷

doi:10.11913/PSJ.2095-0837.2016.30406

拟南芥AtJ3通过核质转运调控植物质膜H⁺-ATPase活性与ABA响应

天水师范学院生物工程与技术学院, 甘肃天水 741000

基金项目:

国家自然科学基金资助项目(31260568,31160060)。

详细信息

作者简介:
赵菲佚(1972-),男,副教授,博士,主要从事植物分子生物学研究。

通讯作者:
赵菲佚,E-mail: tspaulzhao@163.com

中图分类号: Q78
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出版历程
- 收稿日期: 2015-12-03
- 修回日期: 2016-01-04
- 网络出版日期: 2022-10-31
- 发布日期: 2016-06-27

Trafficking of AtJ3 from the Nucleus to the Cytoplasm Regulates Plasma Membrane H⁺-ATPase Activity and ABA Response in Arabidopsis

School of Bioengineering & Biotechnology, Tianshui Normal University, Tianshui, Gansu 741000, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31260568,31160060).

摘要

摘要: 拟南芥AtJ3(Arabidopsis thaliana DnaJ homolog 3)为一蛋白分子伴侣,在植物体内可通过与PKS5(SOS2-like protein kinase 5)蛋白激酶形成复合物来抑制PKS5的活性;同时AtJ3-PKS5复合物可对质膜上H⁺-ATPase质子转运活性进行正向调节,并参与对外源ABA的响应。为揭示AtJ3-PKS5复合物参与质膜H⁺-ATPase活性调节及对外源ABA响应中的作用,本研究以拟南芥AtJ3、PKS5不同突变体为材料,在盐及ABA共同处理下对AtJ3-PKS5复合物的功能及作用机制进行了探讨。结果显示,在2种因素共同处理下,AtJ3-PKS5复合物可同时对处理因素进行响应。即AtJ3-PKS5复合物可对质膜上H⁺-ATPase质子转运活性进行调节,并使细胞内pH值发生变化,同时还可诱导ABI5下游ABA响应基因的表达;外源ABA可引起AtJ3从细胞核向细胞质的转运,从而增强了AtJ3对H⁺-ATPase活性的调节。说明AtJ3-PKS5复合物在对H⁺-ATPase活性调节及对外源ABA响应的交互代谢途径中起着关键调节子的作用。
- AtJ3-PKS5复合物 /
- AtJ3转运 /
- H⁺-ATPase活性 /
- ABA响应
Abstract: Arabidopsis thaliana DnaJ homolog 3 (AtJ3), an Arabidopsis chaperone, interacts with SOS2-like protein kinase 5 (PKS5) to form the AtJ3-PKS5 complex and achieves its function through repressing activity of PKS5 in vivo. Moreover, the AtJ3-PKS5 complex positively regulates plasma membrane H⁺-ATPase activity and is involved in exogenous ABA response in Arabidopsis. In this study, salt- and ABA-treated Arabidopsis AtJ3 and PKS5 mutants were explored to elucidate the function and mechanism of the AtJ3-PKS5 complex in the simultaneous regulation of membrane H⁺-ATPase activity and ABA response. Results showed that the AtJ3-PKS5 complex not only led to changes in the cytosolic pH value via regulation of plasma membrane H⁺-ATPase activity, but also activated expression of the ABA-related responsive genes under the two treatments. Additionally, exogenous application of ABA induced trafficking of AtJ3 from the nucleus to the cytoplasm to enhance H⁺-ATPase activity, indicating that the AtJ3-PKS5 complex functions in ABA-meditated pH homeostasis. Taken together, our results suggest that the AtJ3-PKS5 complex might serve as a key regulator in a crosstalk metabolic pathway of H⁺-ATPase activity regulation and exogenous ABA response.
- AtJ3-PKS5 complex /
- AtJ3 trafficking /
- H⁺-ATPase activity /
- ABA response

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