基于流式细胞术的海菜花属植物基因组大小测定

严东莹; 李艳; 孙文礼; 陈进明; 李治中

doi:10.11913/PSJ.2095-0837.2020.20173

基于流式细胞术的海菜花属植物基因组大小测定

严东莹^1,2,
李艳^3,4,
孙文礼⁵,
陈进明³,
李治中^3,4, ,

1. 西藏大学青藏高原生态与环境研究中心, 拉萨 850000;
2. 西藏大学理学院, 拉萨 850000;
3. 中国科学院武汉植物园, 中国科学院水生植物与流域生态重点实验室, 武汉 430074;
4. 中国科学院大学, 北京 100049;
5. 华中农业大学, 武汉 430074

基金项目:

中国科学院战略性先导科技专项（B类）（XDB31000000）；中国科学院大学生科研实践训练项目。

详细信息

作者简介:
严东莹(1995-),女,硕士研究生,研究方向为水生植物生物地理学(E-mail:yandongying@wbgcas.cn)。

通讯作者:
李治中,E-mail:lizhizhong@wbgcas.cn

中图分类号: Q943.2;Q949.71
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出版历程
- 收稿日期: 2019-09-26
- 修回日期: 2019-11-10
- 网络出版日期: 2022-10-31
- 发布日期: 2020-04-27

Estimation of genome sizes of plants from Ottelia Pers. based on flow cytometry

1. Research Center for Ecology and Environment of Qinghai-Tibetan Plateau, Tibet University, Lhasa 850000, China;
2. College of Science, Tibet University, Lhasa 850000, China;
3. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
4. University of Chinese Academy of Sciences, Beijing 100049, China;
5. Huazhong Agricultural University, Wuhan 430070, China

Funds:

This work was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000) and Student Innovation Training Program of the Chinese Academy of Sciences.

摘要

摘要: 利用改良的裂解液P1，以中国古代莲（Nelumbo nucifera Gaertn.Fruct.et Semin）为外标，采用流式细胞术（FCM）对海菜花属（Ottelia Pers.）6个代表性物种及3个存疑类群的基因组大小（C值）进行测定，并对海菜花属系统发育关系进行评估。结果显示：所测定的材料中，水菜花（Ottelia cordata（Wall.） Dandy）C值最小（6.759 pg），灌阳水车前（O.guanyangensis Z.Z.Li，S.Wu&Q.F.Wang）C值最大（12.929 pg）；对基因组大小与该属系统发育树进行比较分析，结果发现该属植物基因组大小与系统发育关系具有一致性；对海菜花属3个存疑类群进行分子系统学研究，结果发现存疑类群与嵩明海菜花（Ottelia acuminata var. songmingensis Z.T.Jiang，H.Li&Z.L.Dao）及灌阳水车前的关系最近，而与水菜花的关系较远，这与基因组大小变异相一致。根据基因组大小进一步推测3个存疑类群很可能为二倍体。本研究结果可为海菜花属植物的系统学研究提供新资料，同时为该属植物基因组学研究提供基础数据。
- 海菜花属 /
- 基因组大小 /
- 系统发育 /
- 流式细胞术
Abstract: The genome sizes of six taxa and three doubtful species of Ottelia were evaluated using flow cytometry (FCM) with the modified lysate P1. Nelumbo nucifera Gaertn. Fruct. et Semin was used as a reference species. The smallest C-value (6.759 pg) was detected in Ottelia cordata (Wall.) Dandy, whereas the largest C-value (12.929 pg) was found in Ottelia guanyangensis Z. Z. Li, S. Wu & Q. F. Wang. An ambiguous correlation was detected between the phylogenetic relationships within Ottelia and genome size. Furthermore, evaluation of phylogenetic status based on molecular systematics demonstrated that the three doubtful species were diploid and maintained a close relationship with Ottelia acuminata var. songmingensis Z. T. Jiang, H. Li & Z. L. Dao and O. guanyangensis, but a distant relationship with Ottelia cordata. Our study provides basic genomic resources for future phylogenetic and genomic research on the genus Ottelia.
- Ottelia /
- Genome size /
- Phylogeny /
- Flow cytometry

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