水稻黄叶少分蘖突变体<i>yllt1</i>的鉴定及基因定位

康伟伟; 李哲理; 高扬; 戴力; 杨峰; 肖丰; 谭炎宁; 易自力

doi:10.11913/PSJ.2095-0837.2020.50654

水稻黄叶少分蘖突变体yllt1的鉴定及基因定位

康伟伟^1,2,
李哲理^2,3,
高扬⁴,
戴力⁵,
杨峰²,
肖丰³,
谭炎宁^2, ,,
易自力^1, ,

1. 湖南农业大学生物科学技术学院, 长沙 410128;
2. 湖南杂交水稻研究中心杂交水稻国家重点实验室, 长沙 410125;
3. 湖南农业大学农学院, 长沙 410128;
4. 黑龙江省农垦科学院水稻研究所, 黑龙江佳木斯 154025;
5. 湖南省水稻研究所, 长沙 410125

基金项目:

湖南省自然科学基金项目（2019JJ40206）；湖南农业科技创新资金项目（2017XC09）；国家“转基因专项”（2016ZX08001004）；湖南杂交水稻研究中心科研创新基金项目（YB201907）。

详细信息

作者简介:
康伟伟(1994-),女,硕士研究生,研究方向为水稻分子遗传(E-mail:1349014465@qq.com)。

通讯作者:
谭炎宁,E-mail:tyncreatrer@126.com

易自力,E-mail:yizili889@163.com

中图分类号: Q943.2
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出版历程
- 收稿日期: 2020-02-21
- 修回日期: 2020-05-05
- 网络出版日期: 2022-10-31
- 发布日期: 2020-10-27

Identification and gene mapping of yellow-leaf less-tillering mutant yllt1 in rice (Oryza sativa L.)

Kang Wei-Wei^1,2,
Li Zhe-Li^2,3,
Gao Yang⁴,
Dai Li⁵,
Yang Feng²,
Xiao Feng³,
Tan Yan-Ning^2, ,,
Yi Zi-Li^1, ,

1. State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China;
2. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;
3. College of Agronomy Science, Hunan Agricultural University, Changsha 410128, China;
4. Rice Research Institute of Heilongjiang Academy of Agricultural Reclamation Sciences, Jiamusi, Heilongjiang 154025, China;
5. Hunan Provincial Rice Research Institute, Changsha 410125, China

Funds:

This work was supported by grants from the Natural Science Foundation of Hunan Province (2019JJ40206), Hunan Agricultural Science and Technology Innovation Fund Project (2017XC09), National “GMO Special Project” (2016ZX08001004) and Scientific Research and Innovation Fund of Hunan Hybrid Rice Research Center (YB201907).

摘要

摘要: 采用⁶⁰Co-γ射线诱变籼稻（Oryza sativa subsp.indica）保持系‘T98B’获得一份兼具黄叶和少分蘖表型的突变体yllt1（yellow leaf and less tillering 1），利用色素含量测定、构建显隐性混池和基因表达量测定等方法从表型和遗传层面对其遗传特征进行分析。结果显示：yllt1苗期叶绿素a和叶绿素b含量为野生型水稻品种‘T98B’的77.78%和60.00%，叶绿体发育异常，缺乏功能性叶绿体类囊体片层；其分蘖盛期的单株分蘖数为野生型的21.43%。遗传分析发现，在突变体yllt1与‘T98B’的杂交F₂群体中，黄叶与少分蘖性状的重组率为0.00%，表明yllt1同时控制叶色与分蘖表型；yllt1呈隐性遗传，受一个细胞核基因独立控制。该研究进一步采用连锁分析法将yllt1精细定位到第11染色体上，经测序分析推断发生了突变的登录号为LOC_Os11g05552的基因是yllt1的目的基因；该基因编码叶绿体前体信号识别颗粒54 kD（cpSRP54）蛋白，其第1外显子的第29位碱基C发生了缺失，将造成其蛋白产物从N-端至C-端氨基酸组成的严重破坏。RT-qPCR分析结果显示，yllt1叶中叶绿素合成基因OsCAO1、OsCAO2与OsNOL等的表达量明显下调；茎中分蘖正向调控基因OsTAC1受到显著抑制，而负调控基因OsTB1与OsDLT的表达量明显增强。研究结果表明cpSRP54同时参与了水稻叶色和分蘖的调控。
- 水稻 /
- 叶色变异 /
- 分蘖 /
- 黄叶少分蘖突变体 /
- 叶绿体前体信号识别颗粒54(cpSRP54)
Abstract: The phenotypes and genetic control of rice (Oryza sativa L.) mutant yllt1 (yellow leaf and less tillering 1) were analyzed. Results showed that chlorophyll a and chlorophyll b contents in yllt1 were 77.78% and 60.00% that of wild-type ‘T98B’, and abnormal chloroplasts with a few functional thylakoid lamellar were present at the seedling stage. At the vigorous tillering stage, tiller number in yllt1 was 21.43% that of the wild-type. Genetic analysis revealed that the recombination rate between the yellow leaf trait and less tillering trait was 0.00%, based on a F₂ population from yllt1 crossed with ‘T98B’, indicating that the two traits were both controlled by yllt1. Moreover, yllt1 was recessively inherited via a nuclear gene. We fine mapped yllt1 to Chromosome 11 using linkage analysis, and the LOC_Os11g05552 mutant, as verified by sequencing, was identified as a likely candidate for yllt1. It encoded a signal recognition particle 54 kD protein, chloroplast precursor (cpSRP54). In yllt1, deletion of cds. 29 (C) on exon 1 of cpSRP54 would damage the protein structure from the N-terminus to C-terminus. Based on quantitative real-time polymerase chain reaction (qRT-PCR), genes involved in chlorophyll synthesis, including OsCAO1, OsCAO2, and OsNOL, were significantly down-regulated in yllt1. Furthermore, the positive regulator of tillering (OsTAC1) was decreased by 50%, whereas the negative regulators (OsTB1 and OsDLT) were increased 4.5-fold compared with the wild-type. The results herein suggest that cpSRP54 is involved in the regulation of leaf color and tillering. In addition, our results should help to explore the intrinsic relationship between leaf color and tillering dependent on yllt1.
- Oryza sativa /
- Leaf color variation /
- Tillering /
- Yellow leaf and less tillering mutant /
- Chloroplast precursor signal recognition particle 54 (cpSRP54)

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