基于群体转录组解析南荻局域适应的关键因子

乔匿骎; 邢世来; 宋志红; 易自力; 闫娟

doi:10.11913/PSJ.2095-0837.2020.60795

基于群体转录组解析南荻局域适应的关键因子

乔匿骎¹,
邢世来²,
宋志红²,
易自力^1, ,,
闫娟^3, ,

1. 湖南农业大学生物科学技术学院, 长沙 410128;
2. 中国科学院北方资源植物重点实验室, 北京 100049;
3. 中国科学院武汉植物园植物种质改良与特色农业重点实验室, 武汉 430074

基金项目:

国家自然科学基金项目（31971566）；中国科学院青年促进会项目（Y829281G02）。

详细信息

作者简介:
乔匿骎(1994-),女,硕士研究生,研究方向为植物适应性进化(E-mail:823139121@qq.com)。

通讯作者:
易自力,E-mail:yizili@hunan.net

闫娟,E-mail:yanj@wbgcas.cn

中图分类号: Q347
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出版历程
- 收稿日期: 2020-03-22
- 修回日期: 2020-04-07
- 网络出版日期: 2022-10-31
- 发布日期: 2020-12-27

Key factors of Miscanthus lutarioriparius L. Liu ex Renvoize & S. L. Chen local adaptation based on population transcriptomes

1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;
2. Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31971566) and Chinese Academy of Sciences Youth Innovation Promotion Association (Y829281G02).

摘要

摘要: 选取有代表性的10个南荻（Miscanthus lutarioriparius L.Liu ex Renvoize&S.L.Chen）野生居群，以种群转录组数据为基础，对种群局部适应性的关键因子进行研究。首先利用SNP构建推断单倍型，分析环境间和推断单倍型间的差异表达基因，并将这类基因分为4类：仅受环境显著影响的基因（E）、仅受单倍型显著影响的基因（G）、受环境和单倍型相互作用显著影响的基因（G&E）以及非显著影响的基因。分析结果显示：G&E和E基因集在种群局域适应中发挥重要作用，前者的基因表达更容易受其它因素的影响，而后者相对较为稳定；前者中显著富集核糖体途径相关功能基因，后者中显著富集蛋白质折叠相关功能基因。这两类功能基因均参与响应外界压力，推测这些基因的差异表达主要受环境以及基因型和生长环境相互作用的影响。
- 芒属 /
- 转录组 /
- 推断基因型 /
- 局域适应
Abstract: Based on population transcriptomic data of 10 representative populations of Miscanthus lutarioriparius, we studied the key factors impacting local adaptations of the species. We used single nucleotide polymorphisms (SNPs) to phase haplotypes and analyze differentially expressed genes among environments and inferred haplotypes. These genes were then classified into four categories: those significantly affected by the environment (E), significantly affected by haplotypes (G), significantly affected by environmental and haplotype interactions (G & E), and not significantly affected. Based on further analysis, the G & E and E gene sets played importance roles in the local population. The gene expression of G & E was more sensitive to other factors, while the E gene set of was relatively stable. The former was significantly enriched in ribosomal pathway-related functional genes, and the latter was significantly enriched in protein folding-related functional genes. These functional genes were involved in responses to external pressure. Therefore, we inferred that the differential expression of these genes was mainly affected by the environment and the interaction between genotypes and the environment.
- Miscanthus /
- Transcriptome /
- Phased haplotype /
- Local adaptation

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