不同种源中国马褂木镉响应差异及其耐镉能力评估

孙小艳; 吴照祥; 郭雄昌; 伍祎翌; 李彦强

doi:10.11913/PSJ.2095-0837.2021.60663

不同种源中国马褂木镉响应差异及其耐镉能力评估

1. 江西省科学院微生物研究所, 江西省重金属污染生态修复工程技术研究中心, 南昌 330096;
2. 江西省科学院生物资源研究所, 南昌 330096

基金项目:

国家自然科学基金（31760166）；江西省杰出青年人才资助计划（20192BCB23026）；江西省“双千人才”项目（jxsq2019201086）；江西省重点研发计划项目（20171BBG70047）；江西省科学院重点研发项目（2018-YZD1-04）

详细信息

作者简介:
孙小艳(1983-),女,博士,研究员,研究方向为林木抗逆育种与污染土壤修复(E-mail:xiaoyan_sun05@163.com)

中图分类号: Q945.78
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出版历程
- 收稿日期: 2021-05-05
- 修回日期: 2021-06-23
- 网络出版日期: 2022-10-31
- 发布日期: 2021-12-27

Differences in responses of Liriodendron chinense (Hemsl.)Sarg. seedlings from different provenances to cadmium stress and cadmium tolerance evaluation

1. Jiangxi Engineering and Technology Research Center for Ecological Remediation of Heavy Metal Pollution, Institute of Watershed Ecology, Jiangxi Academy of Sciences, Nanchang 330096, China;
2. Institute of Biology and Resources, Jiangxi Academy of Sciences, Nanchang 330096, China

Funds:

supported by grants from the National Natural Science Foundation of China(31760166)

摘要

摘要: 中国马褂木(Liriodendron chinense (Hemsl.)Sarg.)是优良的绿化树种和用材树种，本文以来自3个省份7个种源的中国马褂木实生幼苗为材料，采取水培法，研究10 mg/kg 镉胁迫0、5、10、15 d 后叶片生理生化指标的变化，以及根、茎、叶对镉的吸收累积特征，并对其耐镉能力进行综合评价。结果显示：随着镉胁迫时间延长，各种源幼苗生长均受到抑制，叶绿素含量显著下降，丙二醛(MDA)累积量持续上升，不同种源的变化幅度差异显著；超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均显著升高，不同种源酶活性随胁迫时间的变化趋势不一致。基于生理生化指标，利用隶属函数法评价不同种源马褂木镉耐受能力的强弱顺序为：贵州剑河 > 贵州松桃 > 浙江遂昌 > 云南河口 > 云南麻栗坡 > 贵州道真 > 云南金平。处理15 d后，中国马褂木植株镉累积量从高到低排序为根 > 茎 > 叶，贵州剑河、贵州松桃种源植株中的镉浓度相对较低，而云南河口和云南金平种源植株中的镉浓度显著高于其他种源，不同种源植株镉浓度与隶属函数分析得出的耐镉能力具有较强的相关性。本研究结果表明：各种源中国马褂木耐镉能力差异较大，耐性种源可能通过老叶脱离、激活抗氧化酶活性、根系限制镉离子的吸收转运等方式缓解镉胁迫的毒害，从而表现出较强的耐镉性。
- 中国马褂木 /
- 种源 /
- 镉胁迫 /
- 生理响应 /
- 镉吸收
Abstract: Liriodendron chinense (Hemsl.)Sarg. is a good timber and landscape tree species. In the current study, we investigated the physiological responses of L. chinense seedlings from three provinces and seven provenances under cadmium stress (concentration of 10 mg/kg) at four different time points (0, 5, 10, and 15 d) in hydroponic experiments. The characteristics of Cd²⁺ absorption and distribution in roots, stems, and leaves were measured. Cadmium tolerance (Cd) of the different provenances were evaluated by the membership function method based on the physiological indices of the leaves. Results showed that the growth of seedlings from different provenances was inhibited to varying degrees with prolonged cadmium stress. Chlorophyll content decreased significantly and malondialdehyde content increased, but the change ranges differed significantly among provenances. Superoxide dismutase (SOD) and peroxidase (POD) activities were increased significantly, but the trend patterns in different provenances varied with prolongation of stress time. Based on physiological and biochemical indices, the order of cadmium tolerance of L.chinense from different provenances was: GZJH > GZST > ZJSC > YNHK > YNMLP > GZDZ > YNJP. After 15 d of Cd stress, the order of cadmium concentration in the plants was root > stem > leaf. Furthermore, cadmium concentrations in the roots, stems, and leaves of the GZJH and GZST provenances from Guizhou were significantly lower than that in the YNHK and YNJP provenances from Yunnan. Cadmium tolerance of the L. chinense provenances differed from each other and showed strong correlation with the concentration of cadmium in the plant organs. Seedlings from resistant provenances may alleviate cadmium-induced damage by separating old leaves, regulating antioxidant enzyme activity, and restricting cadmium absorption of the root, which could be used for the ecological restoration of cadmium-polluted soil.
- Liriodendron chinense /
- Provenance /
- Cadmium stress /
- Physiological response /
- Cadmium absorption

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参考文献(28)

[1]	骆永明,滕应,过园.土壤修复———新兴的土壤科学分支学科[J].土壤,2005,37(3):230-235. Luo YM,Teng Y,Guo Y. Soil remediation-a new branch discipline of soil science[J]. Soil,2005,37(3):230-235.
[2]	Zacchini M,Pietruni F,Mugnozza GS,Lori V,Pietrosanti L,Massacci A. Metal tolerance,accumulation and translocation in poplar and willow clones treated with cadmium in hydroponics[J]. Water Air Soil Poll,2009,197(1-4):23-34.
[3]	马晓华,张旭乐,钱仁卷,郑坚,陈义增,张庆良.镉与铜胁迫下无柄小叶榕的生理响应[J].森林与环境学报,2019,39(2):194-200. Ma XH,Zhang XL,Qian RJ,Zheng J,Chen YZ,Zhang QL. Physiological response of Ficus concinna var. subsessilis under heavy metal cadmium-copper stress[J]. Journal of Forest and Environment,2019,39(2):194-200.
[4]	汪有良,王保松,施士争.灌木型柳树镉吸收积累性状的研究[J].西北林学院学报,2011,26(2):105-110. Wang YL,Wang BS,Shi SZ. Cadmium absorption characters of bush willow[J]. Journal of Northwest Forestry University,2011,26(2):105-110.
[5]	周青,黄晓华,施国新,徐雁,戴玉锦.镉对5种常绿树木若干生理生化特性的影响[J].环境科学研究,2001,14(3):9-11. Zhou Q,Huang XH,Shi GX,Xu Y,Dai YJ. Effect of cadmium on the physiological and biochemical character of evergreen trees[J]. Research of Environmental Science,2001,14(3):9-11.
[6]	Granel T,Robinson B,Mills T,Clothier B,Green S,Fung L. Cadmium accumulation by willow clones used for soil conservation, stock fodder and phytoremediation[J].Aust J Soil Res,2002,40(8):1331-1337.
[7]	Das P,Samantaray S,Rout GR. Studies on cadmium toxicity in plants:a review[J]. Environ Pollut,1997,98(1):29-36.
[8]	张大众,杨海川,菅明阳,隗书伟,赵越,张一阳.Cd胁迫下小麦的形态生理响应及Cd积累分布特征[J].农业环境科学学报,2019,38(9):2031-2040. Zhang DZ,Yang HC,Jian MY,Huai SW,Zhao Y,Zhang YY. Physiological response and Cd accumulation and distribution characteristics of wheat under Cd stress[J].Journal of Agro-Environment Science, 2019, 38(9):2031-2040.
[9]	He JY,Ren YF,Zhu C,Yan YP,Jiang DA. Effect of Cd on growth,photosynthetic gas exchange,and chlorophyll fluorescence of wild and Cd-sensitive mutant rice[J].Photosynthetica,2008,46(3):466-470.
[10]	Benvides MP,Gallego SM,Tomaro ML. Cadmium toxicity in plants[J]. Brzailian J Plant Physiol,2005,17(1):21-34.
[11]	徐爱春,陈益泰,王树凤,吴天林.镉胁迫下柳树5个无性系生理特性的变化[J].生态环境,2007,16(2):410-415. Xu AC,Chen YT,Wang SF,Wu TL. Changes of physiological characterstic of five salix clones under cadmium stress[J]. Ecological Environment,2007,16(2):410-415.
[12]	Laureysems I,Blust R,Temmerman LD,Lemmens C,Ceulemans R. Clonal variation in heavy metal accumulation and biomass production in a poplar coppice culture:Ⅰ.Seasonal variation in leaf,wood and bark concentrations[J]. Environ Pollut,2004,131(3):485-494.
[13]	Pietrini F,Zacchini M,Iori V,Pietrosanti L,Bianconi D,Massacci A. Screening of poplar clones for cad-mium phytoremediation using photosynthesis, biomass and cadmium content analyses[J]. Int Journal Phytoremed,2009,12(1):105-120.
[14]	赵志新,乔瑞芳,季孔庶.杂交鹅掌楸不同无性系对Pb胁迫的生理响应及抗性比较[J].植物资源与环境学报,2007,16(4):7-12. Zhao ZX,Qiao RF,Ji KS. Comparison of resistance and physiological response of different clones of hybrid tulip tree(Liriodendron Chinese×L. tulipofera)to Pb stress[J]. Journal of Plant Resources and Environment,2007,16(4):7-12.
[15]	赵志新,乔瑞芳,季孔庶.镉胁迫对不同家系杂交鹅掌楸生长及抗性的影响[J].浙江农林大学学报,2009,26(5):667-673. Zhao ZX,Qiao RF,Ji KS. Cadmium stress on growth and resistance of hybrid tulip trees:Liriodendron Chinese×Liriodendron tulipofera[J]. Journal of Zhejiang Forestry College,2009,26(5):667-673.
[16]	Zhivotovsky OP,Kuzovkina JA,Schulthess CP,Morris T,Ge M. Hydroponic screening of willows(Salix L.)for lead tolerance and accumulation[J]. Int J Phytoremediat,2011,13(1):75-94.
[17]	Sun XY,Hu LX,Xie Y,Fu JM. Evaluation of genotypic variation in heat tolerance of tall fescue by functional traits[J]. Euphytica,2014,199(3):247-260.
[18]	Lomaglio T,Rocco M,Trupiano D,De Zio E,Grosso A,Marra M,et al. Effect of short-term cadmium stress on Populus nigra L. detached leaves[J]. J Plant Physiol,2015,182:40-48.
[19]	Marijke J,Els K,Henk S,Mattijs B,Luis EH,Robert C,et al. Differential response of Arabidopsis leaves and roots to cadmium:glutathione-related chelating capacity vs antioxidant capacity[J]. Plant Physiol Bioch,2014,83:1-9.
[20]	李亚藏,王庆成.镉污染胁迫对4种北方阔叶树苗木膜质过氧化和保护酶活性的影响[J].东北林业大学学报,2007,35(5):24-26. Li YZ,Wang QC. Effects of cadmium stress in soil on activity of membrane lipid peroxidation and protective enzymes in seedlings of four northern broadleaves tree species[J]. Journal of Northeast Forest University,2007,35(5):24-26.
[21]	SoltiÁ,SárváriÉ,Sz9ll'o si E,Tóth B,Mészáros I,et al.Stress hardening under long-term cadmium treatment is correlated with the activation of antioxidative defence and iron acquisition of chloroplasts in Populus[J]. Z Naturforsch C,2016,71(9-10):323-334.
[22]	Ge W,Jiao YQ,Sun BL,Qin R,Jiang WS,Liu DH. Cadmium-mediated oxidative stress and ultrastructural changes in root cells of poplar cultivars[J]. S Afr J Bot,2012,83:98-108.
[23]	Wang J,Yu N,Mu G,Shinwari KI,Shen Z,Zheng L.Screening for Cd-safe cultivars of Chinese cabbage and a preliminary study on the mechanisms of Cd accumulation[J]. Int J Env Res Pub He,2017,14(4):395.
[24]	金雅琴,李冬林,陈小霞,张丽娟.不同种源乌桕幼苗对干旱胁迫的生理响应[J].西北植物学报,2012,32(7):1395-1402. Jin YQ, Li DL, Chen XX, Zhang LJ. Physiological response of Sapium sebiferum seedings from different provenances to drought stress[J]. Acta Botanica BorealiOccidentalia Sinica,2012,32(7):1395-1402.
[25]	王玮琳,刘清泉,张永侠,王银杰,原海燕,黄苏珍.不同种源马蔺幼苗对Cd耐性及积累特征的差异分析[J].植物资源与环境学报,2019,28(2):57-63. Wang WL, Liu QQ, Zhang YX, Wang YJ, Yuan HY,Huang SZ. Analysis on differences in tolerance and accumulation characteristics of Cd of Iris lactea var. Chinensis seedlings from different provenances[J]. Journal of Plant Resources and Environment,2019,28(2):57-63.
[26]	万雪琴,张帆,夏新莉,尹伟伦.镉胁迫对杨树矿质营养吸收和分配的影响[J].林业科学,2009,45(7):45-51. Wan XQ,Zhang F,Xia XL,Yi WL. Effects of cadmium stress on absorption and distribution of mineral nutrients in poplar plant[J]. Science Silvae Sinicae,2009,45(7):45-51.
[27]	Li KQ,Chen L,Feng YH,Yao J,Li B,et al. High genetic diversity but limited gene flow among remnant and fragmented natural populations of Liriodendron chinense Sarg[J]. Bioch Syst Ecol,2014,54:230-236.
[28]	Yang A,Dick CW,Yao X,Huang H. Impacts of biogeographic history and marginal population genetics on species range limits:a case study of Liriodendron chinense[J]. Sci Rep-UK,2016,6(1):25632.