朱红大杜鹃MADS-box基因家族的全基因组鉴定与特征分析

柳文; 马永鹏; 应支萍; 杨琳祥; 谢孟; 马宏

doi:10.11913/PSJ.2095-0837.23363

朱红大杜鹃MADS-box基因家族的全基因组鉴定与特征分析

柳文^{1, 2,},
马永鹏³,
应支萍⁴,
杨琳祥^{1, 2},
谢孟^{1, 2},
马宏^{1, 5, ,}

1.
中国林业科学研究院高原林业研究所，昆明 650233
2.
南京林业大学风景园林学院，南京 210037
3.
云南省极小种群野生植物综合保护重点实验室，中国科学院昆明植物研究所，昆明 650201
4.
西南林业大学园林园艺学院，昆明 650224
5.
国家林业和草原局资源昆虫培育与利用重点实验室，昆明 650233

基金项目: 云南省重大科技计划专项（202302AE090018，202202AE090012）；云南省乡村振兴科技专项（202404BI090014）；兴滇人才支持计划项目（YNWRQNBJ-2019-010）；极小种群野生植物保护项目（2022SJ07X-03）。

详细信息

作者简介:
柳文（1996−），男，硕士研究生，研究方向为杜鹃属植物保护生物学（E-mail：1585957360@qq.com）

通讯作者:
马宏: E-mail：hortscience@163.com

中图分类号: Q943.2
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出版历程
- 收稿日期: 2023-12-03
- 录用日期: 2024-03-11
- 刊出日期: 2024-10-30

Genome-wide identification and characterization of MADS-box gene family of Rhododendron griersonianum Balf. f. et Forrest

Liu Wen^{1, 2,},
Ma Yongpeng³,
Ying Zhiping⁴,
Yang Linxiang^{1, 2},
Xie Meng^{1, 2},
Ma Hong^{1, 5, ,}

1.
Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650233, China
2.
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
3.
Key Laboratory of Comprehensive Conservation for Extremely Small Populations of Wild Plants in Yunnan Province, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
4.
College of Landscape Architecture and Horticulture, Southwest Forestry University, Kunming 650224, China
5.
Key Laboratory of Resource Insect Cultivation and Utilization, State Forestry and Grassland Administration, Kunming 650233, China

摘要

摘要:
MADS-box是在植物生长发育过程中扮演重要角色的一类转录因子，特别是花器官形成、开花时间控制及果实发育与成熟等过程。本研究基于朱红大杜鹃（Rhododendron griersonianum Balf. f. et Forrest）全基因组测序数据，利用生物信息学方法从中鉴定出 81 个 MADS-box 基因，并进行了分析。根据系统发育关系和蛋白结构将 MADS-box分为两类：Type-Ⅰ型包含 24 个基因，Type-Ⅱ型包含57个基因，这些基因不均匀地分布于12条染色体上；81个MADS-box 基因中，存在 6 对片段复制和 1 对串联复制基因，且它们经历了纯化选择作用；同时，基因启动子区域含有光响应、植物生长、激素反应和胁迫响应等顺式作用元件。综上，本研究鉴定了朱红大杜鹃的MADS-box家族转录因子，为深入研究其 MADS-box 蛋白的生物学功能提供了基础。
- 朱红大杜鹃 /
- MADS-box 基因家族 /
- 生物信息学 /
- 基因结构
Abstract:
MADS-box genes play an important role in plant growth and development, especially in processes such as floral organ formation, flowering time regulation, and fruit development and ripening. Based on whole-genome sequencing data of Rhododendron griersonianum Balf. f. et Forrest, 81 MADS-box genes were identified and analyzed using bioinformatics methods. Phylogenetic analysis and protein structure classification divided these genes into two classes, including 24 genes in Type-Ⅰand 57 genes in Type-Ⅱ. The MADS-box genes were unevenly distributed across 12 chromosomes, with no genes located on chromosome 2. Among the 81 MADS-box genes, six pairs showed segmental duplications and one pair showed tandem duplication, all of which have undergone purifying selection. The promoter regions of the MADS-box genes contained elements involved in light response, plant growth, hormone response, and stress response. Overall, the identification of MADS-box gene family members provides a reliable reference for further studies on the biological functions of MADS-box proteins in R. griersonianum
- Rhododendron griersonianum /
- MADS-box gene family /
- Bioinformatics /
- Gene structure
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图 1 拟南芥与朱红大杜鹃 MADS-box 基因家族的系统进化分析

Figure 1. Phylogenetic analysis of MADS-box family in Arabidopsis thaliana and Rhododendron griersonianum

下载: 全尺寸图片幻灯片

图 2 朱红大杜鹃MADS-box基因种内共线性分析

Figure 2. Collinearity analysis of Rhododendron griersonianum MADS-box gene

下载: 全尺寸图片幻灯片

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