MeJA对薰衣草细胞中抗氧化物质合成影响的代谢组学分析

周笑如; 周昱成; 刘学; 刘春环; 杨成

doi:10.11913/PSJ.2095-0837.24104

MeJA对薰衣草细胞中抗氧化物质合成影响的代谢组学分析

江南大学化学与材料工程学院，江苏无锡 214122

基金项目:

江苏省自然科学基金项目（BK20221069）。

详细信息

作者简介:
周笑如（1999−），女，硕士研究生，研究方向为植物组培活性成分（E-mail：xiaoru0901@163.com）

通讯作者:
刘学: E-mail：xueliu@jiangnan.edu.cn

中图分类号: Q942.6
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- 文章访问数: 125
- HTML全文浏览量: 30
- PDF下载量: 10
出版历程
- 收稿日期: 2024-04-18
- 录用日期: 2024-06-21
- 刊出日期: 2025-04-29

Metabolomic analysis of the effects of MeJA on antioxidant compound synthesis in Lavandula angustifolia Mill. cells

School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China

摘要

摘要:
为了探究茉莉酸甲酯（MeJA）对薰衣草（Lavandula angustifolia Mill.）叶片悬浮细胞中抗氧化活性物质合成的影响，采用不同浓度的MeJA处理悬浮细胞，筛选出能够促进抗氧化物质合成的最佳条件，并通过非靶向代谢组学技术分析了MeJA对细胞代谢物合成的作用。结果显示：8 mg/L的MeJA显著抑制了薰衣草叶片悬浮细胞的生长和抗氧化活性；而2 mg/L的MeJA显著促进了抗氧化活性物的合成；与对照组相比，ABTS自由基清除率和FRAP值分别提高了1.30和2.56倍，总酚、总黄酮和总三萜含量分别增加了1.45、1.59和1.24倍。利用超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOF-MS）技术，对未处理组（CK）和2 mg/L MeJA处理组（MJ）进行代谢组学分析，共鉴定出1 403种代谢物，其中151种在两组间有显著差异。差异代谢物涉及177条代谢通路，主要富集于代谢信号转导通路和ABC转运蛋白通路。因此，MeJA可能主要通过影响代谢信号转导和调节ABC转运蛋白的功能影响薰衣草悬浮细胞中抗氧化活性物质的合成。
- 薰衣草 /
- 悬浮培养 /
- 茉莉酸甲酯 /
- 抗氧化 /
- 代谢组学
Abstract:
The effects of methyl jasmonate (MeJA) on the synthesis of antioxidant compounds in Lavandula angustifolia Mill. leaf suspension cells were investigated by introducing varying concentrations of MeJA into the culture medium. The optimal conditions for enhancing antioxidant synthesis were determined, and non-targeted metabolomics was employed to analyze the impact of MeJA on metabolite production. Results demonstrated that 8 mg/L MeJA inhibited both cell growth and antioxidant activity, whereas 2 mg/L MeJA significantly promoted the biosynthesis of antioxidant compounds. Compared with the untreated group, cells treated with 2 mg/L MeJA exhibited a 1.30-fold increase in ABTS radical scavenging activity and a 2.56-fold increase in ferric reducing antioxidant power (FRAP). Additionally, total phenol, flavonoid, and triterpene contents were elevated by 1.45-fold, 1.59-fold, and 1.24-fold, respectively. Metabolomic profiling using UHPLC-Q-TOF-MS identified 1 403 metabolites, with 151 showing significant differences between untreated and MeJA-treated cells. These differential metabolites were associated with 177 metabolic pathways, predominantly enriched in metabolic pathways and ABC transporter pathways. These findings suggest that MeJA modulates antioxidant biosynthesis in L. angustifolia suspension cells primarily by influencing metabolic signal transduction and regulating ABC transporter activity.
- Lavandula angustifolia /
- Suspension culture /
- MeJA /
- Antioxidant /
- Metabolomics

HTML全文

图 1 MeJA对于叶片悬浮细胞生长状态的影响

Figure 1. Effects of MeJA on growth status of leaf suspension cells

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图 2 MeJA对叶片悬浮细胞鲜质量（A）和干质量（B）的影响

Figure 2. Effects of MeJA on fresh (A) and dry weight (B) of leaf suspension cells

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图 3 MeJA对叶片悬浮细胞抗氧化活性的影响

Figure 3. Effects of MeJA on antioxidant activity of leaf suspension cells

*: P<0.05; **: P<0.01; ***: P<0.001.

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图 4 CK和MJ的PCA和OPLS-DA分析

A：CK和MJ的PCA得分散点图；B：CK和MJ的OPLS-DA得分散点图；C：CK和MJ的OPLS-DA模型的置换检验结果。

Figure 4. PCA and OPLS-DA results for CK and MJ

A: Scatterplot of PCA scores of CK and MJ; B: Scatterplot of OPLS-DA scores of CK and MJ; C: Permutation test results of OPLS-DA models for CK and MJ.

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图 5 典型差异代谢物的化学结构

A：倒捻子素；B：槲皮素-3-甲基醚；C：紫丁香苷；D：紫罗兰酮。

Figure 5. Chemical structure of typical differential metabolites

A: Mangostin; B: Quercetin-3-methyl ether; C: Syringin; D: α-Ionone.

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图 6 CK和MJ差异代谢物的含量变化（前20）

红色表示含量上调，蓝色表示含量下降，柱形长度越大，表示差异倍数越大。

Figure 6. Differential metabolite content changes in CK and MJ (top 20)

Red indicates upregulation, blue indicates downregulation, and greater bar length indicates greater multiplicity of variance.

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图 7 CK和MJ的差异代谢物KEGG通路富集

气泡越大，表示该通路中富集到的差异代谢物数量越多；颜色越红，表示差异代谢物在该通路上的富集越显著。

Figure 7. KEGG pathway enrichment analysis of differential metabolites in CK and MJ

Larger bubbles indicate greater amount of differential metabolite enriched in the pathway; redder color indicates more significant enrichment of differential metabolite in the pathway.

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表 1 MeJA对叶片悬浮细胞总酚、总黄酮、总三萜含量的影响

Table 1 Effects of MeJA on total phenol, flavonoid, and triterpene contents in leaf suspension cells

MeJA浓度 Concentration of MeJA / mg/L	总酚含量 Phenol content / mg/g	总黄酮含量 Flavonoid content / mg/g	总三萜含量 Terpenoid content / mg/g
0	54.60±1.08	240.07±32.53	133.45±12.29
0.125	53.05±1.69	284.23±29.26	161.11±15.42
0.25	63.10±1.36**	324.46±16.54*	166.90±4.57*
0.5	59.35±2.48*	277.01±12.29	147.61±1.71
1	63.31±1.59***	330.00±8.41**	188.54±7.08**
2	79.41±1.48***	380.77±0.65**	165.63±10.92*
4	69.59±1.43***	415.46±4.03**	151.95±17.95
8	57.11±0.58*	245.64±19.39	110.19±9.39
Notes: , P<0.05; , P<0.01; **, P<0.001.

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表 2 CK和MJ的生物活性差异代谢物

Table 2 Bioactive differential metabolites of CK and MJ

类别 Category	化合物名称 Compound name	化学式 Chemical formula	加合离子 Adduct	P值 P value	VIP值 VIP value	差异倍数 Fold change
黄酮类	表儿茶素	C₁₅H₁₄O₆	[M+H]⁺	7.10×10⁻⁴	2.41	2.04
	3,4,6-三甲氧基异黄酮-7-O-β-D-吡喃葡萄糖苷	C₂₄H₂₆O₁₁	[M+Na]⁺	9.54×10⁻⁴	1.11	1.74
	丁香亭	C₁₇H₁₄O₈	[M+H]⁺	1.58×10⁻³	1.16	1.80
	栀子黄素B	C₁₉H₁₈O₇	[M+H]⁺	1.04×10⁻²	1.01	0.93
	次野鸢尾黄素	C₂₀H₁₈O₈	[M+Na]⁺	1.06×10⁻⁵	1.65	2.47
	根皮苷	C₂₁H₂₄O₁₀	[M+H]⁺	3.08×10⁻⁵	1.25	2.84
	补骨脂定	C₂₀H₁₆O₅	[M+H]⁺	7.56×10⁻⁵	1.03	1.84
	槲皮素-3-甲基醚	C₁₇H₁₄O₇	[M+H]⁺	1.31×10⁻⁴	1.69	2.97
	大豆苷	C₂₁H₂₀O₉	[M+Cl]⁻	6.64×10⁻³	1.28	0.16
酚酸类	倒捻子素	C₂₄H₂₆O₆	[M+H]⁺	4.62×10⁻⁷	4.01	359.35
	蛇床子素	C₁₅H₁₆O₃	[M+H]⁺	2.49×10⁻³	1.19	2.52
	胡黄连苷II	C₂₃H₂₈O₁₃	[M+H-H₂O]⁺	4.02×10⁻⁵	1.30	0.07
	佛手酚	C₁₁H₆O₄	[M+H]⁺	7.26×10⁻⁴	5.38	1.50
	紫丁香苷	C₁₇H₂₄O₉	[M+Na]⁺	1.48×10⁻³	1.28	4.85
	依托泊苷	C₂₉H₃₂O₁₃	[M+H]⁺	4.96×10⁻³	1.14	3.57
	阿魏酸	C₁₀H₁₀O₄	[M-H]⁻	2.89×10⁻²	3.03	2.53
	凯林	C₁₄H₁₂O₅	[M+H]⁺	6.88×10⁻⁵	1.09	2.09
	状芸香素	C₁₆H₁₄O₃	[M-H]⁻	5.07×10⁻⁵	1.25	1.56
	对香豆酸	C₉H₈O₃	[M-H-H₂O]⁻	4.34×10⁻²	1.03	0.43
	奎尼酸	C₁₁H₉NO₃	[M-H]⁻	2.52×10⁻⁴	1.49	1.34
萜类	紫罗兰酮	C₁₃H₂₀O	[M+H-H₂O]⁺	1.87×10⁻²	1.08	1.69
	儿茶酚	C₆H₆O₂	[M+H-3H₂O]⁺	6.89×10⁻⁴	1.47	2.18
	球姜酮	C₁₅H₂₂O	[M+H-H₂O]⁺	1.88×10⁻²	2.83	1.86
	18β-甘草次酸	C₃₀H₄₆O₄	[M-H]⁻	1.89×10⁻⁴	4.85	2.15
	刺囊酸-3-O-葡萄糖苷	C₃₆H₅₈O₉	[M-H]⁻	4.75×10⁻⁵	2.24	1.93
	人参皂苷F3	C₄₁H₇₀O₁₃	[M+FA-H]⁻	1.72×10⁻²	5.13	0.68
	苜蓿酸+o-hex	C₃₅H₅₄O₁₁	[M-H]⁻	9.41×10⁻⁴	1.82	3.35
	罗莎白素	C₃₆H₅₈O₁₀	[M+Cl-]⁻	4.35×10⁻³	2.21	1.99
生物碱类	14-氨基喜树碱	C₂₀H₁₇N₃O₄	[M+H]⁺	8.18×10⁻⁷	4.40	3.45
	托品酮	C₈H₁₃NO	[M+H]⁺	1.43×10⁻²	2.24	2.46
	长春胺	C₂₁H₂₆N₂O₃	[M+H]⁺	4.03×10⁻⁶	1.29	8.23
醌类	恩贝灵	C₁₇H₂₆O₄	[M-H]⁻	3.60×10⁻³	3.40	1.40
芪类	紫檀芪	C₁₆H₁₆O₃	[M+H]⁺	7.51×10⁻⁴	1.37	1.55

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MeJA对薰衣草细胞中抗氧化物质合成影响的代谢组学分析

作者简介: 周笑如（1999−），女，硕士研究生，研究方向为植物组培活性成分（E-mail：xiaoru0901@163.com）

通讯作者: 刘学: E-mail：xueliu@jiangnan.edu.cn

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Metabolomic analysis of the effects of MeJA on antioxidant compound synthesis in Lavandula angustifolia Mill. cells

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作者简介:
周笑如（1999−），女，硕士研究生，研究方向为植物组培活性成分（E-mail：xiaoru0901@163.com）

通讯作者:
刘学: E-mail：xueliu@jiangnan.edu.cn