高级检索+

费尔干猪毛菜病程相关蛋白基因(SfPR-1)的克隆及在盐胁迫下的表达分析

王艳, 陈西, 杨中敏, 刘登辉

王艳, 陈西, 杨中敏, 刘登辉. 费尔干猪毛菜病程相关蛋白基因(SfPR-1)的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2013, 31(2): 164-170. DOI: 10.3724/SP.J.1142.2013.20164
引用本文: 王艳, 陈西, 杨中敏, 刘登辉. 费尔干猪毛菜病程相关蛋白基因(SfPR-1)的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2013, 31(2): 164-170. DOI: 10.3724/SP.J.1142.2013.20164
WANG Yan, CHEN Xi, YANG Zhong-Min, LIU Deng-Hui. Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress[J]. Plant Science Journal, 2013, 31(2): 164-170. DOI: 10.3724/SP.J.1142.2013.20164
Citation: WANG Yan, CHEN Xi, YANG Zhong-Min, LIU Deng-Hui. Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress[J]. Plant Science Journal, 2013, 31(2): 164-170. DOI: 10.3724/SP.J.1142.2013.20164
王艳, 陈西, 杨中敏, 刘登辉. 费尔干猪毛菜病程相关蛋白基因(SfPR-1)的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2013, 31(2): 164-170. CSTR: 32231.14.SP.J.1142.2013.20164
引用本文: 王艳, 陈西, 杨中敏, 刘登辉. 费尔干猪毛菜病程相关蛋白基因(SfPR-1)的克隆及在盐胁迫下的表达分析[J]. 植物科学学报, 2013, 31(2): 164-170. CSTR: 32231.14.SP.J.1142.2013.20164
WANG Yan, CHEN Xi, YANG Zhong-Min, LIU Deng-Hui. Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress[J]. Plant Science Journal, 2013, 31(2): 164-170. CSTR: 32231.14.SP.J.1142.2013.20164
Citation: WANG Yan, CHEN Xi, YANG Zhong-Min, LIU Deng-Hui. Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress[J]. Plant Science Journal, 2013, 31(2): 164-170. CSTR: 32231.14.SP.J.1142.2013.20164

费尔干猪毛菜病程相关蛋白基因(SfPR-1)的克隆及在盐胁迫下的表达分析

基金项目: 新疆自治区青年自然科学基金资助项目(2012211B06)。This research work was supported by the Youth Foundation of Xinjiang Natural Science Foundation (2012211B06).
详细信息
    作者简介:

    王艳(1978-),女,理学博士,副教授,研究方向为植物抗逆生理生化与分子机制。

    通讯作者:

    王艳, E-mail: wangyan7899@gmail.com

  • 中图分类号: Q781

Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress

  • 摘要: 利用抑制消减杂交法从藜科猪毛菜属盐生植物费尔干猪毛菜(Salsola ferganica)中分离得到了一个盐胁迫响应的cDNA片段,结合SMARTTM RACE技术获得了费尔干猪毛菜病程相关蛋白基因的cDNA,命名该基因为 SfPR-1 (GenBank登录号: JQ670917)。序列分析表明,SfPR-1长817 bp,含有501 bp的阅读框、 65 bp的 5'-UTR 和251 bp的3'-UTR,编码166个氨基酸,分子质量为18.01 kD,理论等电点为9.37。通过BLAST同源序列比对分析,结果显示该基因编码的蛋白与已知甜菜、拟南芥、烟草及玉米的病程相关蛋白PR-1同源性分别为73.6%、57.8%、55.5%和53.9%,且具有PR-1家族特有的6个半胱氨酸保守结构域。半定量RT-PCR和实时荧光定量RT-qPCR分析表明,该基因在盐胁迫后表达呈明显上调,初步推测病程相关蛋白基因SfPR-1可能与费尔干猪毛菜的耐盐性相关。
    Abstract: A cDNA fragment was isolated from Salsola ferganica by suppression subtractive hybridization and its full-length cDNA with 817 bp was cloned by SMARTTM RACE, and was named SfPR-1 gene (GenBank accession number: JQ670917). The SfPR-1 gene consisted of a 501 bp open reading frame encoding 166 amino acids with molecular weight of 18.01 kDa and an isoelectric point of 9.37, a 65 bp 5'-UTR and 251 bp 3'-UTR. The deduced amino acid sequence of SfPR-1 showed high identity of 73.6%, 57.8%, 55.5% and 53.9% to those of pathogen-related protein 1 from Beta vulgaris, Arabidopsis thaliana, Nicotiana tabacum and Zea mays, respectively, and had a conserved six-cysteine motif. Reverse transcriptase PCR and Real-time PCR methods were used to investigate the expression profile of the SfPR-1 gene under salt stress. SfPR-1 showed up-regulated expression patterns under salt treatment. Based on our results, we concluded that the SfPR-1 gene might be involved in salt response and an important component for the salt tolerant pathway in Salsola ferganica.
  • [1] Durrant W E,Dong X.Systemic acquired resis-tance[J].Annu Rev Phytopathol, 2004, 42: 185-209.
    [2] Sato F,Koiwa H,Sakai Y,Kato N,Yamada Y.Synthesis and secretion of tobacco neutral PR-5 protein by transgenic tobacco and yeast[J].Biochem Biophys Res Commum, 1995, 211: 909-913.
    [3] Van Loon L C,Rep M,Pieterse C M J.Significance of inducible defense-related proteins in infected plants[J].Annu Rev Phytopathol, 2006, 44: 135-162.
    [4] Antoniw J F,Ritter C E,Pierpoint W S,Van Loon L C.Comparison of three pathogenesis-related proteins from plants of two cultivars of tobacco infected with TMV[J].J Gen Virol, 1980, 47: 79-87.
    [5] Ohshima M,Matsuoka M,Yamamoto N,Tanaka Y,Kano-Murakami Y,Ozeki Y,Kato A,Harada N,Ohashi Y.Nucleotide sequence of the PR-1 gene of Nicotiana tabacum[J].FEBS Lett, 1987, 225: 243-246.
    [6] Tornero P,Gadea J,Conejero V,Vera P.Two PR-1 genes from tomato are differentially regulated and reveal a novel mode of expression for a pathogenesis-related gene during the hypersensitive response and development[J].MPMI, 1997, 10: 624-634.
    [7] Maleck K,Levine A,Eulgern T,Morgan A,Schmid J,Lawton K A,Dangl J L,Dietrich R A.The transcriptome of Arabidopsis thaliana during systemic acquired resistance[J].Nat Genet, 2000, 26: 403-410.
    [8] Liu Q,Xue Q.Computational identification of novel PR-1-type genes in Oryza sativa[J].J Genet, 2006, 85: 193-198.
    [9] Mitsuhara I,Iwal T,Seo S,Yanagawa Y,Kawahigasi H,Hirose S,Ohkawa Y,Ohashi Y.Characteristic expression of twelve rice PR-1 family genes in response to pathogen infection,wounding,and defense-related signal compounds(120/180)[J].Mol Genet Genomics, 2008, 279: 415-427.
    [10] Li Z T,Dhekney S A,Gray D J.PR-1 gene family of grapevine: a uniquely duplicated PR-1 gene from a Vitis interspecific hybrid confers high level resistance to bacterial disease in transgenic tobacco[J].Plant Cell Rep, 2011, 30: 1-11.
    [11] Alexander D,Goodman R M,Gut-Rella M,Glascock C,Weymann K,Friedrick L,Maddox D,Ahl-Goy P,Luntz T,Ward E,Ryals J A.Increased tolerance to two Oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a[J].Proc Natl Acad Sci USA, 1993, 90: 7327-7331.
    [12] Lawton K,Uknes S,Friedrich L,Gaffney T,Alexander D,Goodman R,M'etraux J P,Kessmann H,Ahl-Goy P,Gut Rella M,Ward E,Ryals J.The molecular biology of systemic acquired resistance//Fritig B,Legrand M,eds.Mechanisms of Plant Defense Responses.The Netherlands: Klu-wer Academic Publishers, 1993: 422-432.
    [13] Hong J K,Hwang B K.Induction of enhanced disease resistance and oxidative stress tolerance by overexpression of pepper basic PR-1 gene in Arabidopsis[J].Physiol Plant, 2005, 124: 267-277.
    [14] Sarowar S,Kim Y J,Kim E N,Kim K D,Hwang B K,Islam R,Shin J S.Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses[J].Plant Cell Rep, 2005, 24: 216-224.
    [15] Bonasera J M,Kim J F,Beer S V.PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora[J].BMC Plant Biol, 2006, 6: 23.
    [16] Linthorst H J,Meuwissen R L,Kauffmann S,Bol J F.Constitutive expression of pathogenesis-related proteins PR-1,GRP,and PR-S in tobacco has no effect on virus infection[J].Plant Cell, 1989, 1: 285-291.
    [17] Yoshikawa M,Tsuda M,Takeuchi Y.Resistance to fungal diseases in transgenic tobacco plants expressing the phytoalexin elicitor releasing factor,b-1,3-endoglucanase,from soybean[J].Naturwissenschaften, 1993, 80: 417-420.
    [18] Tabei Y,Kitade S,Nishizawa Y,Kikuchi N,Kayano T,Hibi T,Akutsu K.Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold(Botrytis cinerea)[J].Plant Cell Rep, 1998, 17: 159-164.
    [19] Aslanzadeh V,Ghaderian M.Homology Modeling and functional characterization of PR-1a protein of Hordeum vulgare subsp.vulgare[J].Biomed Inform, 2012, 8(17): 807-811.
    [20] Hon W C,Griffith M,Mlynarz A,Kwok Y C,Yang D S C.Antifreeze proteins in winter rye are similar to pathogenesis-related proteins[J].Plant Physiol, 1995, 109: 879-889.
    [21] Broekaert W F,Terras F R G,Cammue B P A.Induced and performed antimicrobial protein//Slusarenko A J,Fraser R S S,van Loon L C,eds.Mechanisms of Resistance to Plant Diseases.Dordrecht: Kluwer Academic Publishers, 2000: 371-477.
    [22] Zeier J,Delledonne M,Mishina T,Severi E,Sonoda M,Lamb C.Genetic elucidation of nitric oxide signaling in incompatible plant-pathogen interactions[J].Plant Physiol, 2004, 136: 2875-2886.
    [23] Hamamouch N,Li C,Seo P L,Park C M,Davis E L.Expression of Arabidopsis pathogenesis-related genes during nematode infection[J].Mol Plant Pathol, 2011, 12(4): 355-364.
    [24] 黄俊华.中国猪毛菜属植物的地理分布特点[J].干旱区地理, 2005, 28(3): 325-329.
    [25] Guan B,Jiang G Q,Wang Y X,Wang Z C,Haxim Y,Bao Q,Hu Y Z,Zhang F C,Wang Y.Identification of differentially expressed transcripts involved in the salt-stress response of Salsola ferganica by suppression subtractive hybridization[J].Plant Cell Tiss Organ Cult, 2010, 103: 343-352.
    [26] Van Loon L C,Van Strien E A.The families of pa-thogenesis-related proteins,their activities,and comparative analysis of PR-1 type proteins[J].Physiol Mol Plant Pathol, 1999, 55: 85-97.
    [27] Ferndndez C,Szyperski T.Bruyere P.NMR solution structure of the pathogenesis-related protein p14a[J].J Mol Biol, 1997, 266(3): 576-593.
    [28] 李佳,郑清川,杜林方.烟草病程相关蛋白PR-1a的三维模型和荧光光谱[J].应用与环境生物学报, 2008, 14(4): 466-468.
    [29] Sharma M,Corges-Crua M,Ahern K R,McMullen M,Brutnell T P,Chopra S.Identification of the Pr1 gene product completes the anthocyanin biosynthesis pathway of maize[J].Genetics, 2011, 188(1): 69-79.
    [30] Van Loon L C,Rep M,Pieterse C M J.Significance of inducible defense-related proteins in infected plants[J].Annu Rev Phytopathol, 2006, 44: 135-162.
    [31] Agrawal G K,Jwa N S,Rakwal R.A novel rice(Oryza sativa L.)acidic PR1 gene highly responsive to cut,phytohormones,and protein phosphatase inhibitors[J].Biochem Biophys Res Commun, 2000, 274(1): 157-165.
  • 期刊类型引用(20)

    1. 陈双双, 贺艳群, 项子金, 刘高平, 黄春辉. 江西省不同产地‘红阳’猕猴桃果实品质比较分析. 浙江柑橘. 2025(02) 百度学术
    2. 李宏祥,陈俐,王春发,欧阳艳,李建仁,陈友平. 不同采收期和贮藏温度对猪血桃果实品质的影响. 湖南生态科学学报. 2025(01): 81-89 . 百度学术
    3. 徐阳,常婧,姜冬,潘丕克. 低温贮藏对软枣猕猴桃主要风味品质指标的影响. 林业科技通讯. 2024(01): 90-93 . 百度学术
    4. 申素云,王周倩,张琦,杨洁,韩飞,钟彩虹,王传华,黄文俊. 36份猕猴桃种质资源的果实品质与感官评价分析. 植物科学学报. 2023(04): 540-551 . 本站查看
    5. 黄文俊,王周倩,张琦,杨洁,申素云,钟彩虹. ‘金圆’猕猴桃在两个地区果实生长发育动态变化研究. 植物科学学报. 2023(04): 531-539 . 本站查看
    6. 李巍,石媛真,郭嘉,黄小玉,林香信,张江周,吴良泉. 不同贮藏时间琯溪蜜柚果肉营养及香气成分研究. 食品安全质量检测学报. 2023(17): 185-195 . 百度学术
    7. 陈佳瑜,吴胜平,黄华毅. 基于正交试验的红肉蜜柚优选施肥方案研究. 林业与环境科学. 2023(04): 120-126 . 百度学术
    8. 张群,舒楠,罗赛男,李绍华,李德金. 不同品种猕猴桃贮藏特性的研究. 湖南农业科学. 2022(01): 73-78+83 . 百度学术
    9. 陈双双,贺艳群,徐小彪,陶俊杰,贾东峰,王祝,黄春辉. 江西省不同产地“金艳”猕猴桃果实品质比较分析. 中国南方果树. 2022(02): 113-116 . 百度学术
    10. 杨蕾,洪林,杨海健,王武,汪卉. 不同采收期及贮藏方式对杂柑爱媛28果实品质的影响. 南方农业学报. 2022(04): 1088-1101 . 百度学术
    11. 李岚欣,孙洁,辛奇,赵泽众,刘帮迪,姜微波. 乡村振兴背景下我国猕猴桃产业技术高质量发展分析. 保鲜与加工. 2022(07): 82-90 . 百度学术
    12. 胡光明,黎纯斌,杨斌,王周倩,申素云,李作洲,钟彩虹. 宜昌市72份野生中华猕猴桃果实性状多样性分析与综合评价. 果树学报. 2022(09): 1540-1552 . 百度学术
    13. 张群,舒楠,罗赛男,李绍华,李德金. 不同品种猕猴桃贮藏特性的研究(英文). Agricultural Science & Technology. 2022(03): 1-9+29 . 百度学术
    14. 张维,黄余年,张群,李高阳,朱向荣. 猕猴桃采后贮藏保鲜技术研究进展. 保鲜与加工. 2021(05): 139-145 . 百度学术
    15. 黄文俊,江昌应,陈美艳,刘小莉,张琦,闫春林,钟彩虹. 三个产地猕猴桃品种‘金梅’在低温贮藏及货架期内的采后生理和品质变化. 植物科学学报. 2020(05): 687-695 . 本站查看
    16. 曹森,雷霁卿,马超,巴良杰,吉宁,崔世江,龙扬,王瑞. 3种生物保鲜剂对贵长猕猴桃货架期品质变化的影响. 中国南方果树. 2020(06): 136-140 . 百度学术
    17. 韩飞,黄宏文,李大卫,张琼,姜正旺,刘小莉,钟彩虹. 优质鲜食黄肉猕猴桃新品种‘金圆’的选育及特性分析. 植物科学学报. 2019(02): 171-180 . 本站查看
    18. 马亚红,蔡锦凡,范小娇,邢卓. 猕猴桃储藏过程中品质变化的模拟预测. 现代食品科技. 2019(05): 253-258+303 . 百度学术
    19. 黄文俊,刘小莉,张琦,陈美艳,钟彩虹. 黄肉红心猕猴桃‘东红’果实在不同贮藏方式下的生理和品质变化研究. 植物科学学报. 2019(03): 382-388 . 本站查看
    20. 曹森,马超,黄亚欣,和岳,巴良杰,吉宁,何贵红,王瑞,顾红艳. 1-MCP对猕猴桃后熟品质的影响. 食品与发酵工业. 2019(14): 184-190 . 百度学术

    其他类型引用(5)

计量
  • 文章访问数:  1516
  • HTML全文浏览量:  0
  • PDF下载量:  2430
  • 被引次数: 25
出版历程
  • 收稿日期:  2012-06-25
  • 修回日期:  2012-11-16
  • 发布日期:  2013-04-29

目录

    /

    返回文章
    返回