RP-HPLC法测定美国不同产地野葛根和叶片中葛根素、大豆苷和大豆苷元含量的研究

杨雪芳; 黄伟; 丁建清

doi:10.3724/SP.J.1142.2013.40391

RP-HPLC法测定美国不同产地野葛根和叶片中葛根素、大豆苷和大豆苷元含量的研究

杨雪芳^1,2,
黄伟^1, ,,
丁建清¹

1.
中国科学院水生植物与流域生态重点实验室, 中国科学院武汉植物园, 武汉 430074
2. 中国科学院大学, 北京 100049

基金项目: 中国科学院知识创新工程重要方向项目(KSCX2-EW-J-20)。

详细信息

作者简介:
杨雪芳(1986-),女,硕士研究生,从事野葛入侵机制及生物防治的研究(E-mail:yang_xue_fang1@126.com)。

通讯作者:
黄伟,E-mail:huangwei0519@hotmail.com

中图分类号: R282;R284.1
计量
- 文章访问数: 1436
- HTML全文浏览量: 0
- PDF下载量: 2168
出版历程
- 收稿日期: 2012-11-29
- 修回日期: 2013-04-02
- 发布日期: 2013-08-29

Determination of Puerarin, Daidzin and Daidzein in Roots and Leaves of Pueraria lobata (Wild) Ohwi from United States by RP-HPLC

1.
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
2. University of Chinese Academy of Sciences, Beijing 100049, China

摘要

摘要: 对采自美国的8个野葛种群的异黄酮类物质进行了定量分析。结果表明,叶片中葛根素、大豆苷和大豆苷元含量分别为0.0950、0.698、0.110 mg/g,但不同产地大豆苷和大豆苷元含量存在显著差异。在整个生长季节中,3种异黄酮类物质的含量均在8月份最高。根中3种异黄酮类物质的含量分别为3.664、1.0302、0.3689 mg/g,但不同产地之间无显著差异。此外,美国种群中异黄酮类物质的含量均显著高于已报道的中国种群。本研究为充分利用国内外野葛资源,确定合理采收时间,挖掘野葛叶片的利用潜力奠定了一定的理论基础,为葛藤新药源的开发利用提供了科学依据。
- 野葛 /
- 异黄酮类化合物 /
- 资源收集和评价
Abstract: In this study,we measured three isoflavonoids in kudzu from eight populations in south-eastern United States.The concentrations of puerarin,daidzin and daidzein in the leaves were 0.0950 mg/g,0.698 mg/g and 0.110 mg/g,respectively.The concentration of daidzin and daidzein was significantly different among populations.During the growing season,three isoflavonoids in the leaves were higher in August than in July and September.The concentrations of the three isoflavonoids in the roots were 3.664 mg/g,1.0302 mg/g and 0.3689 mg/g,respectively.There were no significant differences in the three isoflavonoids among populations.Furthermore,the concentrations of the three isoflavonoids in kudzu from US populations were higher than those of Chinese populations,as reported in previous research.This study aimed to provide a theoretic and scientific basis for making full use of kudzu leaves,determining appropriate harvest times and the development and utilization of the puerarin leaves resource.
- Kudzu /
- Isoflavonoids /
- Collection and evaluation

HTML全文

参考文献(34)

[1]	张鹤山,刘洋.葛资源利用现状及发展前景[J].安徽农学通报,2008,14(5):69-70.
[2]	裴莉昕,陈随清,纪宝玉,董诚明,冯卫生.不同产地葛根药材的质量分析[J].中国实验方剂学杂志,2009,15(10):24-26.
[3]	Lin T.Traditional Chinese medicine,e.g.used for relieving gout and rheumatism,includes Chinese yam,kudzu vine root,long pepper,Houttuynia cordata,Torreya grandis,pine nut,desert fruit,Ginkgo,Polygonatum,corncob,and Rhodiola rosea.CN102488842-A[P].13-Jun-2012.
[4]	Rong H J,Stevens J F,Deinzer M L,De Cooman L,De Keukeleire D.Identification of isoflavones in the roots of Pueraria lobata[J].Planta Med,1998,64(7):620-627.
[5]	Zhang D W,Dai S J,Liu W H,Li G H.Chemical constituents from the vines of Pueraria lobata[J].CJNM,2010,8(3):196-198.
[6]	He X,Blount J W,Ge S,Tang Y,Dixon R A.A genomic approach to isoflavone biosynthesis in kudzu (Pueraria lobata)[J].Planta,2011,233(4):843-55.
[7]	Chen C,Chen C,Wang Z,Wang L,Yang L,Ding M,Ding C,Sun Y,Lin Q,Huang X,Du X,Zhao X,Wang C.Puerarin induces mitochondria-dependent apoptosis in hypoxic human pulmonary arterial smooth muscle cells[J].Plos One,2012,7(3):1-9.
[8]	赵瑛,周游,殷惠军,张颖.葛根素对脂肪细胞糖脂代谢作用研究[J].哈尔滨商业大学学报:自然科学版,2006,22(4):5-7.
[9]	Harborne J B,Williams C A.Advances in flavonoid research since 1992[J].Phytoche-mistry,2000,55(6):481-504.
[10]	Waldron G E,Larson B M H.Kudzu vine,Pueraria montana,adventive in southern Ontario[J].Can Field Nat,2012,126(1):31-33.
[11]	Hu Q.Method of processing root of kudzu vine instant vermicelli,comprises e.g.preparing pulp by detecting qualified kudzu root,and self-ripening by modulating uniform slurry is sent to self forming machine.CN102511803-A[P].27-Jun-2012.
[12]	陈雁,肖杨.外来物种葛藤的入侵与防控[J].现代农业科技,2009(22):307.
[13]	Frye M J,Hough-Goldstein J,Kidd K A.Response of kudzu (Pueraria montana var.lobata) to different types and levels of simulated insect herbivore damage[J].Biol Control,2012,61(1):71-77.
[14]	阎秀峰,王洋,李一蒙.植物次生代谢及其与环境的关系[J].生态学报,2007,27(6):2554-2562.
[15]	Schijlen E G,de Vos C H,van Tunen A J,Bovy A G.Modification of flavonoid biosynthesis in crop plants[J].Phytochemistry,2004,65(19):2631-2648.
[16]	周艳,邹学敏,王维芬,王友良,杨丽萍.不同产地大豆与绿豆中异黄酮及矿物质含量的分析[J].微量元素与健康研究,2011,28(3):37-39.
[17]	段琦梅,梁宗锁,杨东风,聂小妮,刘婷.不同产地黄芪药材质量的差异性分析[J].西北农林科技大学学报:自然科学版,2010,38(10):187-191.
[18]	黄丽莉,金小春,朱培林.不同采收期及不同部位的广东紫珠总黄酮含量分析[J].中国林副特产,2009(3):10-12.
[19]	刘逢芹,李宏建,周扬星,李贵海.不同采集时间野葛不同部位总黄酮和葛根素含量比较[J].植物资源与环境学报,2006,15(2):79-80.
[20]	任亚东,朱艳林.不同产地葛根中总黄酮和葛根素的含量测定[J].辽宁中医药大学学报,2008,10(5):160-161.
[21]	鲁守平,隋新霞,孙群,孙宝启.药用植物次生代谢的生物学作用及生态环境因子的影响[J].天然产物研究与开发,2006,18(6):1027-1032.
[22]	张蕾,朱蓉贞,潘扬,马国祥.不同品种及产地的葛根中葛根素含量的比较[J].中国中药杂志,1995,20(7):399-400.
[23]	Wu Y,Wang X,Fan E.Optimisation of ultrasound-assisted extraction of puerarin and total isoflavones from Puerariae lobatae radix (Pueraria lobata (Wild.) Ohwi) with response surface methodology[J].Phytochem Anal,2012,23(5):513-519.
[24]	吴向阳,仰玲玲,仰榴青,邹艳敏,陆继明.RP-HPLC法同时测定野葛的根、茎和叶中葛根素、大豆苷和大豆苷元的含量[J].食品科学,2009,13(14):248-252.
[25]	黄璐琦,陈美兰,肖培根.中药材道地性研究的现代生物学基础及模式假说[J].中国中药杂志,2004,29(6):5-7.
[26]	朱林.影响中药质量的因素[J].才智,2012(21):358.
[27]	蒋妮,覃柳燕,李力,缪剑华.环境胁迫对药用植物次生代谢产物的影响[J].湖北农业科学,2012,51(8):1528-1532.
[28]	刘芬,崔浪军,何刚,杨章民.不同生长季节丹参营养器官中有效成分的动态变化[J].植物科学学报,2011,29(1):93-98.
[29]	黄瑞松,覃冬杰,张鹏,黄必奎,陶宨平,刘婧.广西不同产地和不同采收期大叶钩藤中钩藤碱定量分析[J].中草药,2012,43(1):178-181.
[30]	潘玲玲,任江剑,江建铭.野葛葛根素积累动态的研究[J].中国现代中药,2011,13(9):15-17.
[31]	Frischknecht P M,Schuhmacher K,Muller-Scharer H,Baumann T W.Phenotypic plasticity of Senecio vulgaris from contrasting habitat types:growth and pyrrolizidine alkaloid formation[J].J Chem Ecol,2001,27(2):343-358.
[32]	Kayano S,Matsumura Y,Kitagawa Y,Kobayashi M,Nagayama A,Kawabata N,Kikuzaki H,Kitada Y.Isoflavone C-glycosides isolated from the root of kudzu (Pueraria lobata) and their estrogenic activities[J].Food Chem,2012,134(1):282-287.
[33]	张德武,戴胜军,李贵海,戴军贵.野葛藤茎中的化学成分研究[J].中草药,2011,42(4):1389-1393.
[34]	肖学凤,高岚.HPLC法测定不同产地葛根中葛根素的含量[J].中草药,2011,32(3):220.

施引文献

资源附件(0)

计量

文章访问数: 1436
HTML全文浏览量: 0
PDF下载量: 2168
被引次数: 0

RP-HPLC法测定美国不同产地野葛根和叶片中葛根素、大豆苷和大豆苷元含量的研究

作者简介: 杨雪芳(1986-),女,硕士研究生,从事野葛入侵机制及生物防治的研究(E-mail:yang_xue_fang1@126.com)。

通讯作者: 黄伟,E-mail:huangwei0519@hotmail.com

计量

出版历程

Determination of Puerarin, Daidzin and Daidzein in Roots and Leaves of Pueraria lobata (Wild) Ohwi from United States by RP-HPLC

计量

出版历程

目录

作者简介:
杨雪芳(1986-),女,硕士研究生,从事野葛入侵机制及生物防治的研究(E-mail:yang_xue_fang1@126.com)。

通讯作者:
黄伟,E-mail:huangwei0519@hotmail.com