脂肪族芥子油苷侧链修饰酶基因<em>FMO<sub>GS-OX4</sub></em>表达模式分析

赵宇; 孔稳稳; 沙伟; 李晶

doi:10.3724/SP.J.1142.2013.40406

脂肪族芥子油苷侧链修饰酶基因FMO_GS-OX4表达模式分析

赵宇¹,
孔稳稳²,
沙伟^1, ,,
李晶^2, ,

1.
齐齐哈尔大学生命科学与农林学院分子遗传学研究室, 黑龙江齐齐哈尔 161006
2. 东北农业大学农业生物功能基因重点实验室, 哈尔滨 150030

基金项目: 黑龙江省高等学校科技创新团队建设计划项目(2011TD005);黑龙江省留学归国科学基金项目(LC2009C31)。

详细信息

作者简介:
赵宇(1986-),女,硕士,研究方向为植物遗传学(E-mail:zhaoyu860125@126.om)。

通讯作者:
沙伟,教授,博士生导师,E-mail:shw1129@267.net

李晶,教授,博士生导师,E-mail:lijing@neau.edu.cn

中图分类号: Q946.83⁺⁹
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出版历程
- 收稿日期: 2012-12-09
- 修回日期: 2013-03-31
- 发布日期: 2013-08-29

Expression Pattern of FMO_GS-OX4, a Biosynthetic Gene Involved in Aliphatic Glucosinolate Side-chain Modification

ZHAO Yu¹,
KONG Wen-Wen²,
SHA Wei^1, ,,
LI Jing^2, ,

1.
Molecular Genetics Laboratory, College of Life Sciences and Forestry, Qiqihar University, Qiqihar, Heilongjiang 161006, China
2. Key Laboratory of Agricultural Functional Genes, College of Life Sciences, Northeast Agriculture University, Harbin 150030, China

摘要

摘要: 芥子油苷是一类由氨基酸衍生而来的、在植物抗生物胁迫防御性反应中起重要作用的次生代谢产物,其生物活性与侧链结构密切相关。拟南芥中有5个黄素单氧化酶FMO_GS-OX1-5具有催化芥子油苷侧链上硫原子氧化的活性,使甲基硫烷芥子油苷转变为甲基亚磺酰烷芥子油苷。前期研究工作表明,在5个FMO_GS-OX基因缺失突变体中,除了fmo_gs-ox4外均表现出芥子油苷侧链结构变化的表型。为了深入揭示FMO_GS-OX4的表达特性和它对芥子油苷侧链的修饰作用,利用GFP和GUS为报告基因,系统地分析了FMO_GS-OX4在不同组织中的表达情况。结果表明FMO_GS-OX4主要在花梗、叶片及角果的维管组织中表达,在正常生长条件下,FMO_GS-OX4表达的空间位置与芥子油苷的分布不重叠,因而,酶与底物的分离可能是fmo_gs-ox4没有明显表型的主要原因。
- 拟南芥 /
- 脂肪族芥子油苷 /
- 黄素单氧化酶 /
- 硫氧化 /
- 组织定位
Abstract: Glucosinolates(GSLs)are amino acid-derived secondary metabolites,which play an important role in biotic defense reactions in plants.The bioactivity of GSLs is closely related with its chain structure.There are five Flavin-containing Monooxygenase(FMO)enzymes,FMO_GS-OX1-5,involved in GSL biosynthesis catalyzing the S-oxygenation from Methylthioalkyl GSLs to Methylsulfinylalkyl GSLs.Our previous work showed that all fmo_gs-ox mutants,except fmo_gs-ox4,presented altered GSLs phenotypes.To reveal the expression characteristics and functions in GSLs biosynthesis and chain structure modification,GFP and GUS were used as reporter genes,expression pattern of FMO_GS-OX4 in different tissue was analyzed and results showed that FMO_GS-OX4 was expressed mainly in the vascular tissue of the leaf,stem and silique.Under normal conditions,spatial distribution of FMO_GS-OX4 did not overlap with the distribution of its substrates,which was considered as the reason why fmo_gs-ox4 lacked altered GSLs phenotype.
- Arabidopsis thaliana /
- Aliphatic glucosinolates /
- FMO_GS-OX4 /
- S-oxygenation /
- Tissue localization

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