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不同方法提取组培百里香精油质量及成分的比较分析

徐世千, 李晓东, 张建国

徐世千, 李晓东, 张建国. 不同方法提取组培百里香精油质量及成分的比较分析[J]. 植物科学学报, 2013, 31(6): 609-615. DOI: 10.3724/SP.J.1142.2013.60609
引用本文: 徐世千, 李晓东, 张建国. 不同方法提取组培百里香精油质量及成分的比较分析[J]. 植物科学学报, 2013, 31(6): 609-615. DOI: 10.3724/SP.J.1142.2013.60609
shu
XU Shi-Qian, LI Xiao-Dong, ZHANG Jian-Guo. Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods[J]. Plant Science Journal, 2013, 31(6): 609-615. DOI: 10.3724/SP.J.1142.2013.60609
Citation: XU Shi-Qian, LI Xiao-Dong, ZHANG Jian-Guo. Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods[J]. Plant Science Journal, 2013, 31(6): 609-615. DOI: 10.3724/SP.J.1142.2013.60609
shu
徐世千, 李晓东, 张建国. 不同方法提取组培百里香精油质量及成分的比较分析[J]. 植物科学学报, 2013, 31(6): 609-615. CSTR: 32231.14.SP.J.1142.2013.60609
引用本文: 徐世千, 李晓东, 张建国. 不同方法提取组培百里香精油质量及成分的比较分析[J]. 植物科学学报, 2013, 31(6): 609-615. CSTR: 32231.14.SP.J.1142.2013.60609
shu
XU Shi-Qian, LI Xiao-Dong, ZHANG Jian-Guo. Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods[J]. Plant Science Journal, 2013, 31(6): 609-615. CSTR: 32231.14.SP.J.1142.2013.60609
Citation: XU Shi-Qian, LI Xiao-Dong, ZHANG Jian-Guo. Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods[J]. Plant Science Journal, 2013, 31(6): 609-615. CSTR: 32231.14.SP.J.1142.2013.60609
shu

不同方法提取组培百里香精油质量及成分的比较分析

  • 1.

    华南农业大学农学院, 广州 510642

  • 2. 深圳职业技术学院化生学院, 深圳 518055

基金项目: 十二五国家科技支撑计划项目（2011BAD18B02）资助；深圳市重点科技项目（2109k3070009）资助。
详细信息
    作者简介:

    徐世千（1986- ），女，重庆人，硕士研究生，研究方向为组织培养及次生代谢产物研究（E-mail：xsq308@163.com）。

    通讯作者:

    张建国, E-mail: zhangjg@scau.edu.cn

  • 中图分类号: Q946.85

Comparative Analysis of Essential Oil Quality and Composition from Tissue Culture Seedlings of Thymus vulgaris L. Using Different Extraction Methods

  • 1.

    College of Agriculture, South China Agriculture University, Guangzhou 510642, China

  • 2. School of Applied Biological Engineering, Shenzhen Polytechnic, Shenzhen 518055, China

摘要

    摘要
  • 摘要: 采用水蒸气蒸馏法、有机溶剂萃取法、CO2超临界萃取法3种提取方法提取组培百里香精油，比较分析精油得率、精油化学成分以及相对含量，以期得出最佳提取方法。结果表明：水蒸气蒸馏法提取的百里香精油得率为0.21%，主要化学成分为：百里酚（36.53%）、间伞花烃（14.13%）、松油烯（8.09%）和石竹烯（4.14%）；有机溶剂萃取法提取的精油得率为0.19%，主要化学成分为：1，2-苯二甲酸-单-2-乙基己基酯（55.23%）、百里酚（8.73%）、松油烯（5.23%）；CO2超临界萃取法提取的精油得率为0.27%，主要化学成分为：百里酚（26.68%）、3-苯基-2-丙烯酸-甲酯（21.55%）、间伞花烃（9.69%）。从精油得率、精油质量以及精油主要化学成分综合比较3种方法，水蒸气蒸馏法是提取百里香精油的最佳方法。
    Abstract: The essential oils of Thymus vulgaris were extracted by steam distillation, organic solvent extraction and supercritical CO2 extraction.The amounts,compositions and relative contents of the essential oils extracted from the three methods were compared.The results showed that the amount of essential oils extracted by steam distillation was 0.21%,the main chemical components were thymol(36.53%), benzene,1-methyl-3-(1-methylethyl)-(14.13%),1,4-cyclohexadiene,1-methyl-4-(1-methylethyl)(8.09%), and caryophyllene(4.14%);the amount of essential oils extracted by organic solvent extraction was 0.19%,the main components were 1,2-benzenedicarboxylic acid,mono(2-ethylhexyl)ester(55.23%),thymol(8.73%),and 1,4-cyclohexadiene,1-methyl-4-(1-methylethyl)(5.23%);and the amount of essential oils extracted by supercritical CO2 extraction was 0.27%,the main components were thymol(26.68%),2-propenoic acid,3-phenyl-,methyl ester(21.55%), and benzene,1-methyl-3-(1-methylethyl)(9.69%).Based on the quality,major chemical compositions and extraction amount of essential oils,the steam distillation was the best method to extract essential oils from Thymus vulgaris.
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出版历程
  • 收稿日期:  2013-01-06
  • 修回日期:  2013-04-19
  • 发布日期:  2013-12-29

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