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东亚砂藓组织培养技术方法研究

张梅娟, 沙伟

张梅娟, 沙伟. 东亚砂藓组织培养技术方法研究[J]. 植物科学学报, 2013, 31(6): 616-622. DOI: 10.3724/SP.J.1142.2013.60616
引用本文: 张梅娟, 沙伟. 东亚砂藓组织培养技术方法研究[J]. 植物科学学报, 2013, 31(6): 616-622. DOI: 10.3724/SP.J.1142.2013.60616
ZHANG Mei-Juan, SHA Wei. Research on Tissue Culture Technology of Racomitrium japonicum[J]. Plant Science Journal, 2013, 31(6): 616-622. DOI: 10.3724/SP.J.1142.2013.60616
Citation: ZHANG Mei-Juan, SHA Wei. Research on Tissue Culture Technology of Racomitrium japonicum[J]. Plant Science Journal, 2013, 31(6): 616-622. DOI: 10.3724/SP.J.1142.2013.60616
张梅娟, 沙伟. 东亚砂藓组织培养技术方法研究[J]. 植物科学学报, 2013, 31(6): 616-622. CSTR: 32231.14.SP.J.1142.2013.60616
引用本文: 张梅娟, 沙伟. 东亚砂藓组织培养技术方法研究[J]. 植物科学学报, 2013, 31(6): 616-622. CSTR: 32231.14.SP.J.1142.2013.60616
ZHANG Mei-Juan, SHA Wei. Research on Tissue Culture Technology of Racomitrium japonicum[J]. Plant Science Journal, 2013, 31(6): 616-622. CSTR: 32231.14.SP.J.1142.2013.60616
Citation: ZHANG Mei-Juan, SHA Wei. Research on Tissue Culture Technology of Racomitrium japonicum[J]. Plant Science Journal, 2013, 31(6): 616-622. CSTR: 32231.14.SP.J.1142.2013.60616

东亚砂藓组织培养技术方法研究

基金项目: 国家自然科学基金资助项目(31070180,31270254)。
详细信息
    作者简介:

    张梅娟(1982- ),女,山东省海阳人,博士研究生,从事苔藓植物分子生物学研究(E-mail:zhangmeijuan_002@163.com);沙伟(1963- ),女,黑龙江省齐齐哈尔人,教授,博士生导师,研究方向为苔藓植物遗传学和分子生物学。

    通讯作者:

    沙伟, E-mail: shw1129@263.net

  • 中图分类号: Q943.1

Research on Tissue Culture Technology of Racomitrium japonicum

  • 摘要: 以东亚砂藓(Racomitrium japonicum)配子体为外植体,比较了不同消毒液对消毒效果的影响,对接种消毒外植体的培养基进行了尝试性筛选,并研究了蔗糖浓度对东亚砂藓配子体生长的影响。结果显示,将东亚砂藓配子体用2%洗洁精溶液浸泡数分钟后,再用0.02%的升汞处理45~60 s的消毒效果最佳;有机培养基是无菌外植体接种的最佳培养基;3%的蔗糖更有利于无菌外植体产生的原丝体团和幼嫩配子体的生长。
    Abstract: The effects of different disinfectants on explants of Racomitrium japonicum were studied here. Suitable inoculation media were screened tentatively, and the effects of sucrose concentrations on growth of R. japonicum gametophytes were investigated. The results showed that a concentration of 0.02% HgCl2 for 45-60 seconds was optimal for initiation cultures from fragments of gametophytes pre-immersed in 2% detergent for a period of time. The suitable inoculation medium for sterile explants was organic culture medium MS. Protonemal colonies and young gametophytes then grew well when 3% sucrose was added.
  • [1] 吴鹏程.苔藓植物生物学[M].北京: 科学出版社, 1998: 1-357.
    [2] 黎兴江.中国苔藓志:第3卷[M].北京:科学出版社,2000:54-57.
    [3] Ohta Y,Hirose Y.Induction and characteristics of cultured callus from some liverworts of Jungermanniales[J].J Hattori Bot Lab,1982,53:239-244.
    [4] Hatcher R E.Sporeing development in the genus Lepidolaena[J].J Hattori Bot Lab,1963,26:10-14.
    [5] Mehra P N,Pahwa M S.Phenotypic variations in Fossombronia himalayensis Kash.In vitro-effect of sugars,auxins,growth substances and growth inhibitors[J].J Hattori Bot Lab,1976,40:317-395.
    [6] Sharon E B B.The sporeing ontogeny of Pellia epiphylla (L.) Cord and Pellia neesiana (Gott.) Limpr.With special reference to the protonema[J].J Hattori Bot Lab,1996,79:115-128.
    [7] Sabovljevic M,Bijelovic A,Dragicevic I.In vitro culture of mosses:Aloina aloides (K.F. Schultz) Kindb.,Brachythecium velutinum (Hedw.) B.S.G.,Ceratodon purpureus (Hedw.) Brid.,Eurhynchium praelongum (Hedw.) B.S.G.and Grimmia pulvinata (Hedw.) Sm.Turkish[J].J Bryol,2003,27:441-446.
    [8] Rowntree J K.Development of novel methods for the initiation of in vitro bryophyte cultures for conservation[J].Plant Cell Tiss Organ Cult,2006,87:191-201.
    [9] Vujicic M,Sabovljevic A,Sabovljevic M.Axenically culturing the bryophytes:A case study of the moss Dicranum scoparium Hedw.(Dicranaceae,Bryophyta) [J].Botanica Serbica,2009,33(2):137-140.
    [10] Duckett J Q,Burch J,Fletcher P W,Matcham H W,Read D J,Russell A J,Pressel S.In vitro cultivation of bryophytes:a review of practicalities,problems,progress and promise[J].J Bryol,2004,26:3-20.
    [11] Ahmed M G U,Shin S L,Lee C H.In vitro culture responses of Cratoneuron decipiens (Brid.) G.Roth gametophytes for micropropagation[J].Hort Environ Biotechnol,2011,52(6):614-620.
    [12] 高永超,沙伟,张晗.不同植物生长物质对牛角藓愈伤组织诱导的影响[J].植物生理学通讯,2003,39(1):29-32.
    [13] 李晓毓,吴翠珍,熊源新,姜业芳,杨志平,何琳.尖叶匍灯藓的组织培养及显微观察[J].山地农业生物学报,2006,25(3):217-222.
    [14] ChenY Y,Low Y X,Guo S L,Cao T.Successful tissue culture of the medicinal moss Rhodobrum giganteum and factors influencing proliferation of its protonemata[J].Ann Bot Fenn,2009,46(6):516-524.
    [15] 肖显华,王顺珍,林荣双,郑秋生.植物材料表面消毒方法的改进[J].生物技术,1999,9(1):43-45.
    [16] Luretta D S,Barbara B L,James A L.Bacteria isolated from moss and their effect on moss development[J].Bot Gaz,1981,142(4):512-518.
    [17] Hornsehuh M,Grotha R,Kutsehera U.Epiphytic bacteria associated with the bryophyte Funaria hygrometrica:Effeet of Methylobacterium strains on protonema development[J].Original Paper,2002,682-687.
    [18] 周甜甜.几种藓类植物配子体再生体系的建立[D].曲阜:曲阜师范大学,2009.
    [19] 郎玉卓,阚世超,刘伟才,周金川,熊源新.大羽藓组培初代培养适宜消毒方法的筛选[J].安徽农业科学,2008,36(34):14892-14893.
    [20] 梁红柱,郭晓莉,赵建成.大叶藓属(Rhodobryum)[JP]植物组织培养研究[J].贵州师范大学学报:自然科学版,2010,28(4):21-25.
    [21] Basile D V.A comparison of some macronutrient media used to culture bryophytes[J].Bryologist,1975,78:403-413.
    [22] Sabovljevic A,Sabovljevic M,Grubisic D,Konjevic R.The effect of sugars on development of two moss species (Bryum argenteum and Atrichum undulatum) during in vitro culture[J].Belg Journ Bot,2005,138(1):79-84.
    [23] 张楠,杜宝明,季梦成.细叶小羽藓(Haplocla-dium microphyllum)配子体组织培养的消毒方法及蔗糖浓度筛选[J].浙江大学学报:农业与生命科学版,2012,38(3):288-292.
    [24] Smeekens S,Rook E.Sugar sensing and sugar-mediated signal transduction in plants[J].Plant Physiol,1997,115:7-13.
    [25] Smeekens S.Sugar induced signal transduction in plants[J].Ann Rev Plant Physiol Mol Bio,2000,51:49-81.
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出版历程
  • 收稿日期:  2013-06-08
  • 修回日期:  2013-10-04
  • 发布日期:  2013-12-29

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