Studies on the Pollen Vitality and Storage Capacity in Platanus acerifolia
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摘要: 以30~40年生悬铃木(Platanus acerifolia)的花粉为试材,研究了不同培养基对其萌发的作用,同时探讨了不同贮藏条件和贮藏时间对花粉生活力的影响。结果表明:悬铃木花粉在15%蔗糖+0.01%硼酸的培养基上培养24h后萌发率最高;附加琼脂的固体培养基对花粉的萌发影响不大;50mg/L的赤霉素对悬铃木花粉的萌发没有明显的抑制或促进作用;花粉干燥后在低温4℃下贮藏能保持较长的生活力,比未经干燥25℃和4℃下贮藏分别长35d和20d。Abstract: The effects of different culture media,preservation condition and time on the germination of pollens collected from 30-40 years old Platanus acerifolia tree were studied.The result showed that the pollen germinating percentage reached the highest after incubated for (24 h) on the media supplemented with 15% sucrose and 0.01% Boric acid;solid medium supplemented with agar or 50 mg/L gibberelin(GA3) wielded little influence over pollen germination;the dry pollen preserved under the temperature of 4℃ retained the viability 35 days and 20 days longer than the fresh pollen preserved under the temperature of 25℃ and 4℃,respectively.
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Keywords:
- Platanus acerifolia /
- Pollen /
- Vitality /
- Storage capacity
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