Abstract:
Salicylic acid (SA) treatment can induce significant H
2O
2 burst in
Salvia miltiorrhiza suspension culture cell and its medium alkalinization. In this study, the correlation between SA-induced H
2O
2 burst and medium alkalinization was investigated through NADPH oxidase inhibitor imidazole (IMD), H
2O
2 quenching agent dimethylthiourea (DMTU), plasma membrane H
+-ATPase inhibitor Na
3VO
4 and plasma membrane H
+-ATPase activator fusicoccin (FC). Results showed that (1) H
2O
2 treatment also induced significant medium alkalinization, and IMD and DMTU suppressed the process of SA-induced medium alkalinization, which suggested that H
2O
2 was involved in the process of SA-induced medium alkalinization; (2) SA suppressed the activity of plasma membrane H
+-ATPase, Na
3VO
4 treatment caused rapid medium alkalinization and led to H
2O
2 burst ahead of time, and FC reversed SA-induced medium alkalinization and H
2O
2 accumulation, which suggested that plasma membrane H
+-ATPase regulated the change in medium pH value and medium alkalinization promoted the generation of H
2O
2. Overall, the H
2O
2 level in the
S. miltiorrhiza suspension culture cell and its medium alkalinization level functioned together to respond to induction of salicylic acid.