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烟草microRNA171c的功能分析

Functional Analysis of MicroRNA171c in Tobacco

  • 摘要: MicroRNA是一类长度为20~24碱基的非编码小RNA,调控植物多种生理代谢途径。MicroRNA171(miR171)在拟南芥、大麦和水稻中通过负调控SCL靶基因使植物表现出分枝结构变化和其他一些发育表型,还可调控拟南芥叶绿素的合成代谢。但其他植物miR171的功能仍然未知。为了探明烟草miR171c的功能,本研究根据烟草miR171c序列设计了靶基因模拟物STTM171,通过病毒表达载体在烟草中进行表达,抑制miR171c的活性后观察植物表型变化。结果表明在病毒表达STTM171烟草中,植株出现顶端优势丧失、茎干增多等表型。荧光定量PCR检测到STTM171过表达植株miR171c表达量下降,两个推测的SCL靶基因TC134811TC127385表达量上升,表明miR171c可能在烟草和拟南芥等植物中的功能比较保守,可以通过调控可能的靶基因SCL来调节植物的生长发育。

     

    Abstract: MicroRNAs, which are types of non-coding RNAs 20-24 nt in length, regulate multiple physiological processes in plants. MicroRNA171 negatively targets the SCL gene family in plants, which causes altered branch architecture and other developmental changes in Arabidopsis, barley, and rice. It also regulates the metabolism of chlorophyll synthesis in Arabidopsis. However, the function of microRNA171 is unknown in other plant species. STTM171, the target mimic of miR171c, was designed based on the tobacco miR171c sequence and was overexpressed by a virus vector to investigate the function of miR171c in tobacco. The STTM171 overexpressed plants lost apical dominance and had several shoots. The expression of miR171c was decreased and the putative targeted genes TC134811 and TC127385, which encode SCL proteins, were upregulated in STTM171 overexpressed plants. These results indicate that the function of miR171c is likely conserved among tobacco and Arabidopsis, and it regulates target SCLs to affect developmental processes.

     

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