Phenotypic diversity analysis of leaf traits in cultivated Houttuynia cordata Thunb. populations from Hunan Province
-
摘要: 将采自湖南的20个鱼腥草（Houttuynia cordata Thunb.）居群栽培于同质园内，通过测定9个叶片表型性状和6个叶片相对性状，采用方差分析、变异系数、相关性分析、主成分分析以及聚类分析等方法，对鱼腥草居群叶片形态的变异特征进行研究。结果表明：（1）同质园栽培的20个鱼腥草居群叶片15个表型性状均存在显著差异，居群间差异大于居群内差异；（2）居群内叶性状的平均变异系数变化幅度为5.63%~9.08%，说明居群内多样性程度较低，其中株洲攸县居群（P16）变异系数最高（9.08%），怀化溆浦居群（P3）变异系数最小（5.63%）；（3）15个叶性状的平均变异系数变化幅度为3.71%~10.28%，说明各性状的多样性程度也较低，其中叶面积变异系数最大（10.28%），叶宽与叶中部宽之比（W2/W4）的变异系数最小（3.71%）；（4）相关性分析显示，叶面积与叶宽、叶中部宽、叶长、叶基至叶宽处的距离、叶柄长都呈现出显著正相关，与叶尖夹角呈显著负相关，而与叶基夹角关系不大；（5）叶片15个性状中前3个主成分的贡献率为83.66%，聚类分析可将20个居群划分为心形、小心形、狭心形和大心形4类，呈现出间断分布的地理格局；（6）同质园实验说明叶表型性状多样性主要是由遗传物质决定的，对其多样性分析能为优良种质资源的选育提供理论依据。Abstract: We studied the phenotypic diversity of leaf traits in cultivated Houttuynia cordata Thunb. populations from Hunan Province, China. Nine phenotypic traits and six relative traits of leaves were determined. Variance analysis, coefficient of variation, correlation analysis, principal component analysis (PCA), and cluster analysis were used to study the variation of leaf morphology. Results showed that:(1) There were significant differences in leaf phenotypic characters of the 20 H.cordata populations, and the differences among populations were greater than those within populations; (2) The average coefficients of variation for the 20 populations ranged from 5.63% to 9.08%, indicating low diversity among populations. The Youxian population (P16) had the highest coefficient of variation (9.08%), whereas the Xupu population (P3) had the lowest coefficient of variation (5.63%); (3) The average coefficients of variation for the 15 traits ranged from 3.71% to 10.28%, indicating that leaf trait diversity was low. The coefficient of variation for leaf area was the largest (10.28%) among the leaf characters, whereas the leaf width to leaf middle width ratio (W2/W4) was the smallest (3.71%); (4) Correlation analysis showed that leaf area had a significant positive correlation with leaf width, leaf middle width, leaf length, distance from leaf base to leaf width, and petiole length, a significant negative correlation with leaf tip angle, and no particular relationship with leaf base angle; (5) The cumulative contribution rate of the first three principal components for the 15 characters was 83.66%. By cluster analysis, the 20 populations could be clustered into four groups:heart-shaped, small heart-shaped, narrow heart-shaped, and large heart-shaped. These cluster results showed a discontinuous distribution pattern; (6) The common garden experiment showed that the diversity of leaf phenotypic traits was mainly determined by genetic material, and the diversity analyses could provide a theoretical basis for the breeding of excellent germplasm resources.
-
Keywords:
- Houttuynia cordata /
- Common garden experiment /
- Leaf /
- Phenotypic diversity /
- Cluster analysis
-
-
[1] 尹明宇, 姜仲茂, 朱绪春, 包文泉, 赵罕, 乌云塔娜. 内蒙古山杏种群表型变异[J]. 植物生态学报, 2016, 40(10):1090-1099. Yin MY, Jiang ZM, Zhu XC, Bao WQ, Zhao H, Wuyun TN. High-level phenotypic variations in populations of Armeniaca sibirica in Nei Mongol, China[J]. Chinese Journal of Plant Ecology, 2016, 40(10):1090-1099.
[2] 文珊娜, 仲崇禄, 姜清彬,陈羽, 张勇, 李清莹. 灰木莲种源幼苗叶片性状表型多样性分析[J]. 植物研究, 2017, 37(2):288-297. Wen SN, Zhong CL, Jiang QB, Chen Y, Zhang Y, Li QY.Phenotypic diversity analysis of seedling leaf traits of Manglietia conifea Dandy[J]. Bulletin of Botanical Research, 2017, 37(2):288-297.
[3] 宋杰, 李世峰, 刘丽娜, 李树发, 解玮佳, 关文灵. 云南含笑天然居群的表型多样性分析[J]. 西北植物学报, 2013, 33(2):272-279. Song J,Li SF, Liu LN, Li SF, Xie WJ, Guan WL. Phenotypic diversity of natural populations of Michelia yunnanensis[J]. Acta Botanica Boreali-Occidentalia Sinica, 2013, 33(2):272-279.
[4] 李因刚, 柳新红, 马俊伟, 石从广, 朱光权. 浙江楠种群表型变异[J]. 植物生态学报, 2014, 38(12):1315-1324. Li YG, Liu XH, Ma JW, Shi CG, Zhu GQ. Phenotypic variations in populations of Phoebe chekiangensis[J]. Chinese Journal of Plant Ecology, 2014, 38(12):1315-1324.
[5] Pengelly JJL, Sirault XRR, Tazoe Y, Evans JR, Furbank RT, von Caemmerer S. Growth of the C4 dicot Flaveria bidentis:photosynthetic acclimation to low light through shifts in leaf anatomy and biochemistry[J]. J Exp Bot, 2010, 61(14):4109-4122.
[6] Jiang CD, Wang X, Gao HY, Shi L, Chow WS. Systemic regulation of leaf anatomical structure, photosynthetic performance, and high-light tolerance in Sorghum[J]. Plant Physiol, 2011, 155(3):1416-1424.
[7] 王常顺, 汪诗平. 植物叶片性状对气候变化的响应研究进展[J]. 植物生态学报, 2015, 39(2):206-216. Wang CS, Wang SP. A review of research on responses of leaf traits to climate change[J]. Chinese Journal of Plant Ecology, 2015, 39(2):206-216.
[8] 郭卫红, 王华, 虞木奎, 吴统贵, 韩有志. 沿海地区水杉叶片性状的纬度变化机制[J]. 应用生态学报, 2017, 28(3):54-60. Guo WH, Wang H, Yu MK, Wu TG, Han YZ.Latitude variation mechanism of leaf traits of Metasequoia glyptostroboides in eastern coastal China[J]. Chinese Journal of Applied Ecology, 2017, 28(3):54-60.
[9] 蒋艾平, 姜景民, 刘军. 檫木叶片性状沿海拔梯度的响应特征[J]. 生态学杂志, 2016, 35(6):1467-1474. Jiang AP, Jiang JM, Liu J. Responses of leaf traits of Sassafras tsumu (Hemsl.) Hemsl. along an altitudinal gradient[J]. Chinese Journal of Ecology, 2016, 35(6):1467-1474.
[10] 武瑞鑫, 邵新庆, 钟梦莹, 潘多, 李超群, 张德罡. 青藏高原东缘披针叶黄华叶片形态特征对海拔变化的响应[J]. 草原与草坪, 2016, 36(2):1-7. Wu RX, Shao XQ, Zhong MY, Pan D, Li CQ, Zhang DG. Response of leaf morphology traits of Thermopsis lanceolata to elevation gradient in the Qinghai-Tibet Plateau[J]. Grassland and Turf, 2016, 36(2):1-7.
[11] 侯珺, 肖菲菲, 张延龙, 牛立新, 闫奕心. 中国野生百合叶表皮特征及系统学意义[J]. 西北植物学报, 2015, 35(8):1560-1573. Hou J, Xiao FF, Zhang YL, Niu LX, Yan YX. Structural characters of leaf epidermis and their systematic significance in the 20 wild Lilium species of China[J]. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(8):1560-1573.
[12] 樊香绒, 力志, 楚海家, 李伟, 刘艳玲, 陈媛媛. 菱属野生植物形态学性状的可塑性变异及其分类学价值[J]. 植物科学学报, 2016, 34(3):340-351. Fan XR, Li Z, Chu HJ, Li W, Liu YL, Chen YY. Analysis of morphological plasticity of Trapa L. from China and their taxonomic significance[J]. Plant Science Journal, 2016, 34(3):340-351.
[13] 廖保生, 宋经元, 谢彩香,韩建萍, 陈士林. 道地药材产地溯源研究[J]. 中国中药杂志, 2014, 39(20):3881-3888. Liao BS, Song JY, Xie CX, Han JP, Chen SL. Study on traceability system of genuine medicinal materials[J].China Journal of Chinese Materia Medica, 2014, 39(20):3881-3888.
[14] 徐艳琴, 蔡婉珍, 胡生福, 黄小虎, 葛菲, 王瑛. 箭叶淫羊藿同质园栽培居群非腺毛多样性及其分类学启示[J].生物多样性, 2013, 21(2):185-196. Xu YQ, Cai WZ, Hu SF, Huang XH, Ge F, Wang Y. Morphological variation of non-glandular hairs in cultivated Epimedium sagittatum (Berberidaceae) populations and implications for taxonomy[J]. Biodiversity Science, 2013, 21(2):185-196.
[15] 李超, 赵广东, 王兵, 史作民, 邓宗富, 夏晨, 陈和东. 中亚热带樟科3种植物幼苗叶结构型性状的种间差异及其相关性[J]. 植物科学学报, 2016, 34(1):27-37. Li C, Zhao GD, Wang B, Shi ZM, Deng ZF, Xia C, Chen HD. Interspecific differences and correlation among leaf structural traits of three plant seedings of Lauraceae in the mid-subtropical zone of China[J]. Plant Science Journal, 2016, 34(1):27-37.
[16] 陈婧, 方建国, 吴方建, 施春阳, 熊苗苗, 王文清. 鱼腥草抗炎药理作用机制的研究进展[J]. 中草药, 2014, 45(2):284-289. Chen J, Fang JG, Wu FJ, Shi CY, Xiong MM, Wang WQ. Research progress on anti-inflammatory mechanism of Houttuynia cordata[J]. Chinese Traditional and Herbal Drugs, 2014, 45(2):284-289.
[17] Lin TY, Liu YC, Jheng JR, Tsai HP, Jan JT, Wong WR, Horng JT. Anti-enterovirus 71 activity screening of Chinese herbs with anti-infection and inflammation activities[J]. Am J Chin Med, 2009, 37(1):143-158.
[18] Tian LM, Zhao Y, Guo C, Yang XB. A comparative study on the antioxidant activities of an acidic polysaccharide and various solvent extracts derived from herbal Houttuynia cordata[J]. Carbohyd Polym, 2011, 83(2):537-544.
[19] Lai KC, Chiu YJ, Tang YJ, Lin KL, Chiang JH, Jiang YL, et al. Houttuynia cordata Thunb. extract inhibits cell growth and induces apoptosis in human primary colorectal cancer cells[J]. Anticancer Res, 2010, 30(9):3549-3556.
[20] Lau KM, Lee KM, Koon CM, Cheung CS, Lau CP, Ho HM, et al. Immunomodulatory and anti-SARS activities of Houttuynia cordata[J]. J Ethnopharmacol, 2008, 118(1):79-85.
[21] Lee JS, Kim IS, Kim JH, Kim JS, Kim DH, Yun CY. Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response[J]. J Ethnopharmacol, 2008, 117(1):34-40.
[22] 李爱民, 徐刚红, 沈美珍. 鱼腥草的花粉活力及雌雄配子体的发育[J]. 植物科学学报, 2014, 32(6):561-566. Li AM, Xu GH, Shen MZ.Pollen viability and male and female gametophyte development in Houttuynia cordata Thunb.[J]. Plant Science Journal, 2014, 32(6):561-566.
[23] 孙贵佳, 权秋梅, 廖咏梅, 陈劲松, 黎云祥. 不同生境鱼腥草形态特征及主要有效成分含量差异分析[J]. 广西植物, 2014, 34(3):408-413. Sun GJ, Quan QM, Liao YM, Chen JS, Li YX. Analysis on morphological characteristics and effective composition contents of Houttuynia cordata in different habitats[J]. Guihaia, 2014, 34(3):408-413.
[24] 杭悦宇, 黄春洪, 穆森, 徐珞珊. 盾叶薯蓣叶片形态多样性研究[J]. 云南植物研究, 2004, 26(4):398-404. Hang YY, Huang CH, Mu S, Xu LS.Studies on diversity of morphological characteristics of leaves from Dioscorea zingiberensis[J]. Acta Botanica Yunnanica, 2004, 26(4):398-404.
[25] 冯梅, 黄文娟, 李志军. 胡杨叶形变化与叶片养分间的关系[J]. 生态学杂志, 2014, 33(6):1467-1473. Feng M, Huang WJ, Li ZJ.Relationship of leaf shape change and leaf nutrient contents in Populus euphratica[J]. Chinese Journal of Ecology, 2014, 33(6):1467-1473.
[26] 李任辉, 赵仲辉, 李家湘. 基于Photoshop软件测量植物叶面积的白纸背景法[J]. 湖南林业科技, 2016, 43(1):138-142,148. Li RH, Zhao ZH, Li JX.An Photoshop software based and white paper as background method for measuring leaf area[J]. Hunan Forestry Science & Technology, 2016, 43(1):138-142, 148. [27] 周妍慧, 贾瑞冬, 杨树华,赵鑫, 葛红, 罗文美, 赵新梅, 桑益恒. 杏黄兜兰居群表型多样性分析[J]. 园艺学报, 2016, 43(7):118-128. Zhou YH, Jia RD, Yang SH, Zhao X, Ge H, Luo WM, Zhao XM, Sang YH. Phenotypic diversity of Paphiopedilum armeniacum populations[J].Acta Horticulturae Sinica, 2016, 43(7):118-128.
[28] 苏应雄, 张雪, 王文礼,赵云勇, 王跃华, 申仕康. 红棕杜鹃不同海拔种群的表型多样性研究[J]. 西北植物学报, 2017, 37(2):356-362. Su YX, Zhang X, Wang WL, Zhao YY, Wang YH, Shen SK. Phenotypic diversity of Rhododendron rubiginosum populations at different altitudes[J]. Acta Botanica Boreali-Occidentalia Sinica, 2017, 37(2):356-362.
[29] 杨贺雨, 卫海燕, 桑满杰,尚忠慧, 毛亚娟, 王小蕊, 刘芳, 顾蔚. 华中五味子叶表型可塑性及环境因子对叶表型的影响[J]. 植物学报, 2016, 51(3):43-55. Yang HY, Wei HY, Sang MJ, Shang ZH, Mao YJ, Wang XR, Liu F, Gu W. Phenotypic plasticity of Schisandra sphenanthera leaf and the effect of environmental factors on leaf phenotype[J]. Chinese Bulletin of Botany, 2016, 51(3):43-55.
[30] 刘晓, 岳明, 任毅. 独叶草叶片性状表型多样性研究[J]. 西北植物学报, 2011, 31(5):861-867. Liu X, Yue M, Ren Y.Morphologic variation of Kingdonia uniflora leaves[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(5):861-867.
[31] 黄春燕, 吴卫, 郑有良,黄曜. 鱼腥草光合蒸腾特性及影响因素的分析[J]. 西北植物学报, 2006, 26(5):989-994. Huang CY, Wu W, Zheng YL, Huang Y.Photosynthetic and transpiration characteristics and their affecting factors of Houttuynia cordata[J]. Acta Botanica Boreali-Occidentalia Sinica, 2006, 26(5):989-994.
[32] 吴卫, 郑有良, 陈黎,魏育明, 颜泽洪, 杨瑞武. 鱼腥草种质资源的RAPD分析[J]. 药学学报, 2002, 37(12):986-992. Wu W, Zheng YL, Chen L, Wei YM, Yan ZH, Yang RW. RAPD analysis on the germplasm resources of herba Houttyniae[J]. Acta Pharmaceutica Sinica, 2002, 37(12):986-992.
[33] 黎晓英, 魏麟, 伍贤进, 饶力群. 中国不同地理居群鱼腥草遗传多样性分析[J]. 中草药, 2010, 41(2):285-288. Li XY, Wei L, Wu XJ, Rao LQ.Analysis on genetic diversity of different geographical populations of Houttuynia cordata[J]. Chinese Traditional and Herbal Drugs, 2010, 41(2):285-288.
[34] 钟军, 王坤, 仇萍,曾维军, 熊兴耀. 鱼腥草不同地理群体遗传结构与变异的研究[J]. 草业学报, 2011, 20(2):227-233. Zhong J, Wang K, Qiu P, Zen WJ, Xiong XY. Genetic structure and variation in different geographical populations of Houttuynia cordata[J]. Acta Prataculturae Sinica, 2011, 20(2):227-233.
[35] 林美珍, 蔡扬帆, 马燕, 田惠桥. 鱼腥草花药发育的组织化学[J]. 中国药学杂志, 2011, 46(22):1711-1714. Lin MZ, Cai YF, Ma Y, Tian HQ. Phytohistochemistry on developing anthers of Houttuynia cordata[J].Chinese Pharmaceutical Journal, 2011, 46(22):1711-1714.
[36] 胡龙兴, 王兆龙. 植物无融合生殖相关基因研究进展[J]. 遗传, 2008, 30(2):155-163. Hu LX, Wang ZL.Progress on the research of apomixis related genes in plant[J]. Hereditas, 2008, 30(2):155-163.
计量
- 文章访问数: 1017
- HTML全文浏览量: 0
- PDF下载量: 1296
下载:
