Abstract: Heterotrimeric GTP-binding proteins, which are comprised of Gα, Gβ, and Gγ subunits, are evolutionarily conserved signal transduction molecules in eukaryotes. In plants, heterotrimeric G not only plays an important regulatory role in growth and development, but also participates in the regulation of multiple abiotic stress responses. This review focuses on the roles and possible regulation mechanisms of heterotrimeric G in the abiotic stress responses of plants, and puts forward prospects for future research. This paper will provide reference for further studies on the detailed regulation mechanism of heterotrimeric G proteins in the abiotic stress responses of plants.