曲穗草属天然杂种的鉴定及形成研究

侯晨曦; 翟星光; 吴丹丹; 周永红; 张昌兵; 张海琴

doi:10.11913/PSJ.2095-0837.2022.10011

曲穗草属天然杂种的鉴定及形成研究

侯晨曦²,
翟星光²,
吴丹丹^2,4,
周永红^2,4,
张昌兵^3, ,,
张海琴^1,4, ,

1. 四川农业大学草业科技学院, 成都 611130;
2. 四川农业大学小麦研究所, 成都 611130;
3. 四川省草原科学研究院, 成都 610000;
4. 西南作物基因资源发掘与利用国家重点实验室, 成都 611130

基金项目:

国家自然科学基金（31870309、31670331）；阿坝州科技局项目（R21YYJSYJ0014）；四川省科技厅项目（2021NZZJ0010）。

详细信息

作者简介:
侯晨曦(1991-),女,硕士研究生,研究方向为植物系统与进化(E-mail:690133057@qq.com)。

通讯作者:
张昌兵,c.b.zhang@126.com

张海琴,haiqinzhang@163.com

中图分类号: S543⁺.9
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出版历程
- 收稿日期: 2021-08-08
- 修回日期: 2021-12-08
- 网络出版日期: 2022-10-31
- 发布日期: 2022-02-27

Identification and formation of natural hybrids of Campeiostachys in Triticeae

1. Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;
2. Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China;
3. Sichuan Academy of Grassland Science, Chengdu 610000, China;
4. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31870309, 31670331), Aba PrefectureScience and Technology Bureau Project (R21YYJSYJ0014), and Sichuan Province Science and Technology Department Project(2021NZZJ0010).

摘要

摘要: 本研究通过形态学、细胞学、系统发育分析等方法对小麦族曲穗草属（Campeiostachys）的两株疑似天然杂种进行鉴定，探究其可能的亲本及杂种形成机制。结果显示：两株天然杂种在形态上与短芒曲穗草（Campeiostachys breviaristata（Keng） Y.H.Zhou，H.Q.Zhang et C.R.Yang.）相近，长势优于后者，但其花粉育性（13.30%和21.20%）和结实率（10.70%和18.60%）较低；细胞学分析表明两株天然杂种均为六倍体（2n =42），染色体组组成为StStYYHH；系统发育分析结果表明两株杂种的可能亲本为短芒曲穗草、无芒披碱草（Elymus submuticus Keng）、黑紫披碱草（Elymus atratus （Nevski） Hand.-Mazz）。基于杂种具有无芒或短芒的特征，结合形态学特征，推测短芒曲穗草和无芒披碱草为两株天然杂种可能的亲本供体，且短芒曲穗草为母本。
- 曲穗草属 /
- 天然杂种 /
- DMC1 /
- rps16
Abstract: In this study, morphological, cytological, and phylogenetic analyses were used to identify two suspected natural hybrids in the genus Campeiostachys. Results showed that the two natural hybrids were similar to C.breviaristata (Keng) Y. H. Zhou, H. Q. Zhang et C. R. Yang. morphologically, but had low pollen fertility (13.30% and 21.20%) and seed setting (10.70% and 18.60%). Cytological studies showed that the two hybrids were hexaploids (2n=42), with StStYYHH genomes. Phylogenetic analysis based on the nuclear gene DMC1 and chloroplast gene rps16 showed that the possible parents of the natural hybrids were C. breviaristata,Elymus submuticus Keng, and Elymus atratus (Nevski) Hand.-Mazz, with C. breviaristata being the female parent. Considering the hybrids had no or short awns, we inferred that C. breviaristata (female parent) and E. submuticus may be the parental species of these two natural hybrids.
- Campeiostachys /
- Natural hybrids /
- DMC1 /
- rps16

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