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香樟全基因组WRKY基因家族的鉴定与分析

Identification and analysis of the WRKY gene family in whole genome of Cinnamomum camphora(L.) Presl.

  • 摘要: WRKY转录因子基因家族是植物特有的一类基因,在植物次生代谢、生物和非生物胁迫中起着重要的调节作用。本研究通过生物信息学方法,在香樟(Cinnamomum camphora (L.) Presl.)全基因组中鉴定了60个WRKY基因(CcWRKY),并将其分为Group Ⅰ~ Ⅲ,其中,Group Ⅰ和Ⅲ的成员发生了收缩现象;片段复制是CcWRKY基因扩张的主要驱动力;Group Ⅰ有完整的WRKY结构域和锌指基序,但Group Ⅱ、Ⅲ存在结构域和锌指基序的丢失和变异现象;CcWRKY基因的启动子区域具有激素类和胁迫类响应顺式作用元件;基因表达分析结果显示,在贫瘠环境(未施肥)中大多数CcWRKY基因在香樟各个组织中高表达,而环境适宜(施肥)条件下,基因表达量降低。

     

    Abstract: The WRKY transcription factor gene family is a class of plant-specific genes that play important regulatory roles in plant secondary metabolism and biotic and abiotic stresses. Through bioinformatic analysis, 60 WRKY genes were identified in the whole genome of Cinnamomum camphora (L.) Presl., which were divided into Groups Ⅰ,Ⅱ, and Ⅲ. Genes in Group Ⅰ and Group Ⅲ were contracted, while segment duplication was the main cause of CcWRKY gene expansion. The C terminus and N terminus of Group Ⅰ contained a complete WRKY domain and zinc finger motif, however there is loss and variation of the domain and zinc finger motif in members of Group Ⅱ and Group Ⅲ. There were many hormone- and stress-responsive cis-acting elements on the promoter of CcWRKYs, such as ABA, MeJA, SA, drought induction, and anaerobic-related stress response. Expression analysis showed that most CcWRKY genes were highly expressed in various tissues in barren environment (without fertilization), while under suitable environmental (fertilization) conditions, the level of gene expression decreased. This study analyzed the expression patterns of CcWRKY genes in response to abiotic stress and provided a theoretical basis for the subsequent functional verification of the C. camphora WRKY gene.

     

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