Abstract:
MAP70 family members were screened and identified using bioinformatics based on the genomic database
of Brassica napus L., with bioinformatics analysis performed on the sequence characteristics of the identified
BnaMAP70 gene family members. Expression levels of the
BnaMAP70 genes were analyzed using qRT-PCR in seedling varieties with waterlogging sensitivity and tolerance. In total, 19 members of the
BnaMAP70 gene family were identified, distributed on 11 chromosomes and divided into five subfamilies. The upstream elements involved in anaerobic stress and phytohormones of the
BnaMAP70 promoter indicated that they may be involved in plant growth, development, and regulation of waterlogging stress. The transcription levels of genes were species-specific, plant site-specific, and time-specific. The qRT-PCR results showed that
BnaMAP70-1 and
BnaMAP70-4 were differentially expressed under waterlogging stress, indicating that these two genes may be regulated by waterlogging stress.