高级检索+
许文杰, 娄千, 陈启桢, 胡开治, 曹敏, 刘燕琴, 韩蓉蓉, 宋经元. 基于全基因组的栀子花香成分合成TPS基因系统分析[J]. 植物科学学报. DOI: 10.11913/PSJ.2095-0837.23017
引用本文: 许文杰, 娄千, 陈启桢, 胡开治, 曹敏, 刘燕琴, 韩蓉蓉, 宋经元. 基于全基因组的栀子花香成分合成TPS基因系统分析[J]. 植物科学学报. DOI: 10.11913/PSJ.2095-0837.23017
Systematic Analysis of TPS Genes for the Floral Scent Biosynthesis in Gardenia jasminoides Based on Whole Genome[J]. Plant Science Journal. DOI: 10.11913/PSJ.2095-0837.23017
Citation: Systematic Analysis of TPS Genes for the Floral Scent Biosynthesis in Gardenia jasminoides Based on Whole Genome[J]. Plant Science Journal. DOI: 10.11913/PSJ.2095-0837.23017

基于全基因组的栀子花香成分合成TPS基因系统分析

Systematic Analysis of TPS Genes for the Floral Scent Biosynthesis in Gardenia jasminoides Based on Whole Genome

  • 摘要: 栀子花花色洁白且香气怡人,不仅具有极高的观赏价值,还是日常生活重要的天然香料来源。萜类是栀子花独特香气的主要组成成分,但合成该类挥发性产物的关键萜类合成酶TPS尚未鉴定。本研究基于栀子高质量基因组,通过转录组数据、系统发育树和保守结构域分析等,全面挖掘栀子萜类花香成分合成TPS基因。结果显示,栀子基因组共注释到44个TPS基因,不均匀分布于11条染色体,共被划分为5个亚家族,其中27个GjTPS基因属于被子植物特有的TPS-a、TPS-b和TPS-g分支。结合栀子5个器官的转录组数据及实时荧光定量PCR分析,筛选出盛开期花器官特异性高表达的5个候选GjTPS基因,均含有TPS执行催化功能所需的DDXXD和NSE/DTE 活性基序。其中,GjTPS1、GjTPS2、GjTPS3和GjTPS27为TPS-b分支酶,推测为芳樟醇或罗勒烯等栀子主要花香成分合成酶,而TPS-a分支的GjTPS18可能是金合欢烯合成酶。研究结果为栀子萜类花香基因TPS的功能表征提供支撑,推动栀子花香成分合成调控研究。

     

    Abstract: Gardenia jasminoides flowers are white in color and smell pleasant, which have high ornamental value and are an important source of natural spices in daily life. Terpenes are the main components of the unique aroma of G. jasminoides, but the key terpene synthase (TPS) for the biosynthesis of such volatile products has not been identified. Here, based on the high-quality genome of G. jasminoides, the analyses of transcriptome data, phylogenetic tree and conserved domain were used to fully explore TPS genes related to the floral scent biosynthesis. The results showed that 44 TPSs were identified in G. jasminoides, which were unevenly distributed on 11 chromosomes. All GjTPSs were clustered into five subfamilies, and 27 GjTPSs belonged to the angiosperm-specific clades, including TPS-a, TPS-b and TPS-g. Combined with the transcriptome data and quantitative real-time PCR (qRT-PCR) analyses of five organs, five candidate GjTPS genes with high expression in blooming flower were screened, and all of them contained the active motif DDXXD and NSE/DTE necessary for TPS to perform catalytic functions. Among them, GjTPS1, GjTPS2, GjTPS3 and GjTPS27 were grouped in TPS-b clade, which were presumed to be TPS for biosynthesizing the main floral scent components in G. jasminoides such as linalool and ocimene, while GjTPS18 from clade TPS-a may be farnesene synthase. The results provide support for the functional characterization of TPS genes catalyzed the terpene products in flowers and promote the research on the regulation of floral scent biosynthesis in G. jasminoides.

     

/

返回文章
返回