Abstract: VQ (VQ Motif-Containing Proteins) is a kind of plant-specific protein, which plays an important role in plant growth and development and resistance to biotic and abiotic stress, but there are few reports on VQ gene in perennial ryegrass (Lolium perenne L.). In this study, 52 LpVQ genes were identified in the genome of perennial ryegrass by bioinformatics methods, which were unevenly distributed on 7 chromosomes of ryegrass. Cell localization prediction showed that LpVQ protein was mainly located in the nucleus, only LpVQ45 was located in the cell membrane. Further phylogenetic analysis showed that LpVQ was divided into 7 subfamilies and there were only 4 pairs of lineal homologous genes with Arabidopsis thaliana. Based on the gene structure analysis of the LpVQ family, it was found that 88.46% of the LpVQ gene had no intron. Finally, the quantitative analysis of LpVQ under dark, high temperature and salt stress showed that the randomly selected LpVQ genes from each subfamily responded to dark, high temperature and salt stress in varying degrees, and responded quickly in the early stage of stress. This study laid a theoretical foundation for further study on the function of VQ family genes of perennial ryegrass under abiotic stress.