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软枣猕猴桃种质叶和花表型性状多样性研究

Phenotypic diversity analysis of leaf and floral traits in Actinidia arguta (Sieb. & Zucc) Planch. ex Miq. germplasm

  • 摘要: 本研究以国家猕猴桃种质资源圃(中国科学院武汉植物园)内保存的65份软枣猕猴桃(Actinidia arguta (Sieb. & Zucc) Planch. ex Miq.)及14份近缘种的种质为材料,对15个数量性状及20个质量性状进行调查,并结合多样性指数、变异系数、相关性分析和主成分分析等方法开展表型多样性研究。结果显示:质量性状的辛普森指数和香农-维纳指数分别为0.05~0.77和0.14~1.55,两者变化趋势相似。大多数质量性状多样性较低,优势表型集中,叶片多呈卵圆形、基部圆形、叶尖尾状、叶背无茸毛且无白粉,花朵多为单生、花瓣基部重叠、花药黑色、花柱及花丝白色。供试猕猴桃种质数量性状变异丰富,除萼片数量(5.03%)和花瓣数量(6.35%)外,其余性状的变异系数均高于10%,其中叶片面积(25.53%)变异系数最大。相关性分析结果显示,15个数量性状间呈极显著相关的有42对,呈显著相关的有12对。聚类分析将79份种质划分为4个表型特征差别明显的类群,多数黑蕊猕猴桃(A. melanandra Franch.)聚集于第Ⅲ类群。主成分分析将15个叶、花数量性状降维提取为5个主成分,累计贡献率为82.043%,主要反映叶片大小、花器大小、叶柄长等变异信息。研究结果表明,叶片面积、花冠直径、子房横径、叶柄长/叶片长共4个性状指标可作为软枣猕猴桃叶片、花朵的综合评价指标。

     

    Abstract: This study investigated phenotypic diversity in 65 Actinidia arguta (Sieb. & Zucc) Planch. ex Miq. accessions and 14 accessions of closely related taxa maintained at the National Kiwifruit Germplasm Repository, Wuhan Botanical Garden, Chinese Academy of Sciences. A total of 15 quantitative and 20 qualitative traits were evaluated using diversity indices, coefficients of variation (CVs), correlation analysis, cluster analysis, principal component analysis (PCA), and complementary statistical approaches. Results showed that the Simpson and Shannon-Wiener indices for qualitative traits ranged from 0.05 to 0.77 and from 0.14 to 1.55, respectively, and exhibited broadly concordant variation. Diversity was low for most qualitative traits, with several dominant phenotypes. Leaves were generally ovate, with rounded bases and caudate apices, and the abaxial surface was typically glabrous and devoid of bloom. Flowers were predominantly solitary, with overlapping petal bases, black anthers, and white styles and filaments. Among quantitative traits, CVs exceeded 10% for all traits except sepal number (5.03%) and petal number (6.35%), and leaf area showed the greatest variation (25.53%). Correlation analysis identified 42 highly significant and 12 significant trait associations. Cluster analysis separated the 79 accessions into four phenotypically distinct groups, with most A. melanandra Franch. accessions assigned to Group Ⅲ. PCA resolved five principal components that together explained 82.043% of the total variance, with major contributions from leaf size, floral organ dimensions, and petiole length. Leaf area, corolla diameter, ovary transverse diameter, and the ratio of petiole length to leaf length were further identified as informative indicators for integrated evaluation of leaf and floral phenotypes in A. arguta. These findings define major dimensions of phenotypic variation within the evaluated germplasm and provide a quantitative basis for standardized identification, characterization, and description of A. arguta genetic resources.

     

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