Abstract: Sequence-related amplified polymorphism (SRAP) was employed to analyze gene-tic relationships of 5 species of Pinellia in China. With 38 SRAP primer combinations,752 loci were identified, of which 628 were polymorphic and accounted for 83.51% of total amplified loci, showing a high polymorphism among Pinellia in China. The genetic similarity among the 5 species ranged from 0.6513 to 0.7312. Based on the results of cluster and principal coordinate analysis, the 5 species were divided into two groups. P.ternata, P.cordata, P.integrifolia, and P.peltata were clustered into group Ⅰ, while P.pedatisecta was in group Ⅱ. Group Ⅰ comprised two subgroups, namely subgroup A and B.Both P.ternata and P.integrifolia were clustered into subgroup A, while P.cordata and P.peltata were clustered into subgroup B. These results suggest that P.cordata had the closest genetic relationship with P.peltata, and P.ternata was closely related to P.integrifolia. Pinellia pedatisecta was a sister group to the other four species. The results of this research might be useful for guiding exploitation and preservation of Pinellia in China.