Abstract: Tissue culture and regeneration of the medicinal plant, Perilla frutescens, were investigated from different explants with different concentrations and combinations of hormones, and an efficient regeneration system was established. Explants showed high to low regeneration in the order terminal bud, lamina, cotyledon and hypocotyl. The regeneration rate of terminal bud was above 85% with or without hormone, and the addition of appropriate hormone promoted the proliferation rate. The regeneration rate of lamina was 70% on average with 0.05-0.1 mg/L TDZ. Optimal hormone combinations of cotyledon and hypocotyl were MS+KT 1.0 mg/L + IAA 1.0 mg/L and MS + 6-BA 1.0 mg/L + IAA 1.0 mg/L, with regeneration rates of 22.22% and 14.61%, respectively. The best rooting medium was 1/2 MS medium without hormone, leading to rooting rate of 86.67% and root length of 7.36 cm, on average. Explants from lamina could form leaf clusters, which induced adventitious buds repeatedly and proliferated quickly.