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植物生长调节剂对鹿角杜鹃扦插繁殖的影响

Effects of Plant Growth Regulator on Cutting Propagation of Rhododendron latoucheae

  • 摘要: 以腐殖土为基质,研究了5种植物生长调节剂的4个浓度处理、以及萘乙酸(NAA)不同浓度与浸泡时间处理对鹿角杜鹃(Rhododendron latoucheae)当年生枝条扦插繁殖12个育苗指标的影响及各指标间的相关性,并运用隶属函数法对各处理组合的育苗效果进行了综合评价。结果表明:5种生长调节剂中,赤霉素(GA3)、吲哚丁酸(IBA)处理在大部分育苗指标上表现良好,为鹿角杜鹃扦插育苗的理想调节剂,吲哚乙酸(IAA)效果次之,6-苄氨基嘌呤(6-BA)、NAA处理的育苗指标表现较差;4个浓度水平上,愈伤率、腐烂率及老叶留存率总体以低浓度处理最佳,随着浓度升高效果下降;其它9个指标对浓度的响应因生长调节剂种类而异。各指标相关性分析发现,4个根系生长指标,即不定根数、最长不定根长、总根数及根系直径之间呈极显著正相关,但它们与愈伤率、腐烂率、生根率及老叶留存率、新梢率间的相关性较弱。隶属函数综合评价表明,50 mg/L GA3处理为鹿角杜鹃扦插育苗的最佳组合,其次为100 mg/L GA3和400 mg/L IBA处理。本研究为杜鹃花产业化育苗提供了科学依据。

     

    Abstract: Using humus as a substrate, annual branch cuttings of Rhododendron latoucheae were treated with five plant growth regulators under four concentrations. In addition, the cuttings were also treated by naphthalene acetic acid (NAA) under different concentrations and soaking times. The effects of different treatment on cutting propagation were individually evaluated and the correlations of 12 propagation traits were studied. The propagation effects of each treatment combination were evaluated by the subordinate function method. The experiments showed that: gibberellic acid (GA3) and indole-3-butyric acid (IBA) had positive effects on most propagation traits, followed by indole-3-acetic acid (IAA), 6-benzyladenine (6-BA) and NAA, suggesting that GA3 and IBA could be ideal plant growth regulators for cutting propagation of R.latoucheae. Among the four concentrations of growth regulators, the lowest was generally the best treatment for callusing rate, rotting rate and retention rate of old leaves; however, as the concentration increased, it became less effective. The optimal concentrations for the other nine propagation traits varied among the five growth regulators. Correlation analysis of the propagation traits showed that the number of adventitious roots, length of longest adventitious roots, number of total roots and diameter of the root system had significant positive correlations, but little correlation with callusing rate, rotting rate, rooting rate, retention rate of old leaves and new shoot rate. Subordinate function evaluation showed that 50 mg/L GA3 was the best treatment combination for cutting propagation of R.latoucheae, followed by 100 mg/L GA3 and 400 mg/L IBA. This study can provide a scientific basis for the industrialization of rhododendron.

     

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