Abstract: Using polyacrylamide gel electrophoresis(PAGE)to anlysis seed protein ingredients of 12 cultivars from four cultivated species in genus Cucurbita:C.moschato Duch,C.maxima Duch,C.pepo L.,C.ficifolia.The results showed:Among seed proteins extracted with water,0.5 mol·L^-1 NaCl,0.1 mol·L^-1 Lacetic acid and 70% Ethanol,most of them are salt-soluble proteins in C.ficifolia;In the other three cultivated species,most of them are water-soluble proteins,with little salt-soluble proteins;All of the four species have very little acid-soluble proteins;In the examination,no alcohol-soluble protein bandings were obtained.The maps of water-soluble proteins,salt-soluble proteins,acid-soluble proteins showed great difference among the four species,but there were almost no difference among the cultivars of the same specie.