Isolation of a New Low-molecular-weight Glutenin Subunit Gene in Wheat
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摘要: 以新疆小麦日喀则基因组DNA为模板,采用Glu-D3位点特异引物进行PCR扩增。PCR产物插入pUCm-T载体,转化感受态大肠杆菌E.coliDH5α,获得阳性克隆。经测序发现该插入片段长度1287bp,包括了部分启动子序列和完整的编码序列。编码序列推导的蛋白质含有8个半胱氨酸残基,属于6类LMW-GS中的TypeⅠ类,为以后这类基因的功能研究提供了基因材料。Abstract: PCR was used as a rapid alternative method to isolate allelic of wheat low molecular weight subunit(LMW-GS) genes.The primers specific to Glu-D3 locus were designed based on published LMW-GS gene sequence data.The PCR products were inserted into pUCm-T vector and transferred into E.coli DH5α.The size of PCR product was about 1287 bp,including partial promoter and whole coding sequences.The LMW-GS encoded by cloned gene in Xinjiang wheat,Rikeze,contained 8 Cys residuces,being grouped into TypeⅠ.This gene clone was applied in the gene function research by the method of gene engineering.
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Keywords:
- Wheat /
- Low molecular weight subunit /
- Gene isolation
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期刊类型引用(1)
1. 王海霞,解婷婷,米雪,肖路臣,单立山. 干旱荒漠区三种典型灌丛植物凋落物混合分解效应研究. 草地学报. 2024(03): 869-878 . 百度学术
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