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汤华, 向福英, 柳晓磊, 帅爱华. 植物铝离子胁迫的研究方法综述[J]. 植物科学学报, 2007, 25(5): 494-499.
引用本文: 汤华, 向福英, 柳晓磊, 帅爱华. 植物铝离子胁迫的研究方法综述[J]. 植物科学学报, 2007, 25(5): 494-499.
TANG Hua, XIANG Fu-Ying, LIU Xiao-Lei, SHUAI Ai-Hua. A Review of Research Methods on Plant Aluminum Stress[J]. Plant Science Journal, 2007, 25(5): 494-499.
Citation: TANG Hua, XIANG Fu-Ying, LIU Xiao-Lei, SHUAI Ai-Hua. A Review of Research Methods on Plant Aluminum Stress[J]. Plant Science Journal, 2007, 25(5): 494-499.

植物铝离子胁迫的研究方法综述

A Review of Research Methods on Plant Aluminum Stress

  • 摘要: 酸性土壤上,植物的生长受到很大的抑制,铝对植物的毒害是最主要的问题。为了揭示植物铝离子胁迫反应及其耐铝性的机理,科学家们进行了大量研究,取得了许多喜人的进展。根据前人研究,对植物铝离子胁迫的研究方法进行了综述。在试验材料的处理方面介绍了大田栽培、室外盆栽、室内盆栽、营养液栽培等方法;在耐铝性鉴定方面介绍了受害症状、根相对伸长率等直接鉴定法和化学染色、有机酸分泌量等间接鉴定法;在铝离子胁迫基因研究方面,介绍了基因定位技术、基因差异表达研究技术、蛋白质差异表达研究技术及转基因技术等研究方法。

     

    Abstract: The growth of plant was strongly inhibited in acid soil,and aluminum toxicity was the most important reason.Scientists have done extensive research work on aluminum-stress responses and aluminum-tolerant mechanism of plant for a long time,and have made lots of remarkable progress.In this article,a review about research methods of plant aluminum-stress was made.For material treatment methods of experiment,it introduced field cultivating,pot-planting outdoors,pot-planting indoors,and nutrient solution culture.For aluminum-tolerance identification,it introduced direct-identification methods including toxic symptoms,root relative elongation and so on,indirect-identification methods such as chemical staining and organic acid secretion.In gene research of aluminum-stress,many methods were introduced about gene mapping,gene differential expression,protein differential expression,transgenic techniques,and so on.

     

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