The study dealt with extraction of DNA with improved CTAB(cetyl-trimethyl-ammonium bromide) method from Ageratina adenophora
Spreng.It showed that CTAB buffer supplemented with 2% PVP40(polyvinylpyrrolidone 40) and 0.4% β-mercaptoethanal was suitable for the genomic DNA extraction of A.adenophora
value of 1.7-1.9,representing high purity of the extracted DNA and the complete elimination of protein,phenol,polyhexose and RNA.Thus the improved CTAB method was approved suitable for further AFLP analysis of A.adenophora
.Some key factors affecting DNA extraction,restriction-ligase reaction,pre-amplification and amplification processes were studied and an optimized AFLP reaction system of A.adenophora
was established.With this AFLP reaction system,clear DNA electrophoresis fingerprints of A.adenophora
was obtained,and the reproducibility was high.