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珍稀濒危蕨类植物东方水韭的遗传多样性

Genetic Diversity in the Rare and Endangered Fern Isoetes orientalis

  • 摘要: 采用ISSR分子标记对珍稀濒危蕨类植物东方水韭和中华水韭4个自然居群共72个样品进行了DNA多态性分析。从65个随机引物中筛选出11个有效引物,共产生98条DNA片段,其中68条为多态性条带,多态位点百分率(PPL)为66.33%。东方水韭两个居群的多态位点百分比率(PPL)较低(8.16%和7.14%)。中华水韭两个居群的PPL也较低(9.18%和23.47%)。中华水韭物种水平上的PPL(51.02%)高于东方水韭(9.18%)。AMOVA分析结果表明,东方水韭遗传变异中主要存在于居群内(94.74%),而中华水韭绝大部分的遗传变异却存在于居群间(86.71%)。东方水韭和中华水韭个体间UPGMA聚类结果表明:同一物种的个体完全能聚在一起,中华水韭的两个居群也能明显分开,而东方水韭两居群的个体并不能完全聚在一起。探讨了可能造成上述居群遗传结构模式的主要因素,同时提出今后工作中需重点解决的问题。

     

    Abstract: ISSR markers were used to measure genetic diversity of 72 individuals from four natural populations of Isoetes orientalis and I.sinensis,two rare and endangered fern species in China.A total of 98 DNA fragments were scored from the 11 ISSR primers and 66.33% percentages of polymorphic loci(PPL) was found.Lowlevel genetic diversity within both I.orientalis(8.16% and 7.14%) and I.sinensis populations(9.18% and 23.47%) was detected in the present study.AMOVA analysis indicated that for I.orientalis most of the variance(94.74%) resided within populations and for I.sinensis a greater amount of variation(86.71%) resided between populations.UPGMA cluster indicated that the individuals from two different species were distinct to each other.For I.orientalis the individuals for different populations did not cluster together.UPGMA cluster analysis also showed that there was distinct genetic differentiation between populations of I.sinensis.The probable causes that have contributed to the current pattern of genetic structure were discussed and the suggestions on the future protection of the Isoetes species were also given in the present study.

     

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