马尾松染色体荧光带型的研究
STUDY ON CHROMOSOME FLUORESCENT BANDING PATTERN IN PINUS MASSONIANA
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摘要: 对马尾松有丝分裂中期染色体荧光带纹的分析结果表明,其色霉素A的染色体的荧光带型为:1对为着丝粒区和臂间区均有带纹的中间着丝粒染色体;6对为臂间区有带纹的中间着丝粒染色体;1对为着丝粒区有带纹的中间着丝粒染色体;3对无带纹的中间或近中着丝粒染色体;1对为着丝粒区有带纹的近中着丝粒染色体。而DAPI的染色体的荧光带型为:3对为着丝粒和臂间区均有带纹的中间着丝粒染色体;1对在臂间区有带纹的中间着丝粒染色体;6对为着丝粒区有带纹的中间着丝粒染色体;2对无带纹的中间或近中着丝粒染色体。最后对荧光方法的实验方法和应用进行了讨论。Abstract: Root-tip metaphase chromosomes of Pinus massoniana were sequentiallystained with CMAand DAPI. P. massoniana had a pair of metacentric chromosomes withtwo CMA-bands at proximal and interstitial regions,six pairs of metacentric chromosome with interstitial CMA-band, a pair of metacentric chromosome with proximal CMA-band, and three pairs of metacentric or submetacentric chromosome without CMA-band as well as a pair of submetacentric chromosome with proximal CMA-band.But the DAPIbanding pattern was different from CMA banding pattern. P. masoniana had three pairs of metacentric chromosomes with two DAPI-bands at proximal and interstitial regions, a pair of metacentric chromosome with interstitial DAPI-band, sixpairs of metacentric chromosome with proximal DAPI-band,and two pairs of metacentric or submetacentric chromosome without DAPL-band.
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Keywords:
- Pinus massoniana /
- Fluorescent banding /
- Chromosome
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